Biotek Synergy LX, User Manual

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46 | Chapter 3 : Getting Started
You can create an Absorbance, Fluorescence, or Luminescence protocol to read a
Take3 plate, independent of the predefined Take3 protocols. Choose the plate type
"Take3" when selecting the protocol parameters.
Define a Take3 Plate
For all new Take3 plates, you must enter the serial number, add pathlength values, align
the plate, and select protocol options. After these steps have been completed, the Take3
tab appears on the Main Menu and contains all pre-programmed Take3 protocols.
This procedure ensures that the Take3 slide's microspot positions are captured correctly in
the reader's software.
Ensure that the glass slide in the plate is completely clean before running the
alignment process. Refer to the
Take3/Take3 Trio User Guide for cleaning
instructions.
If you remove or replace the glass slide, perform the Pathlength Calibration
procedure described in the
Take3/Take3 Trio User Guide and then run the alignment
procedure described below. Update the Pathlength Values on the touchscreen with
the new values generated through the Pathlength Correction procedure described in
the user guide.
The reader can be configured with only one Take3 plate at a time. The plate is
identified by its serial number.
1. From the Main Menu, tap Instrument , then the Take3 tab.
2. Enter the Take3 plate’s serial number, and place the Take3 plate on the carrier. Tap
Required and then Start to perform the alignment process. Note that this process can
take several minutes.
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.