Biotek Synergy H1, Operator'S Manual

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It is the user’s responsibility to understand the volumetric limits of the plate type in use
as it applies to the assay being run.
Luminescence Measurements
For highly sensitive Luminescence assays using white plates, add a Delay step to your
Procedure to “dark adapt” the plates in the reading chamber before taking
measurements.
Monochromator-Based Fluorescence Systems
Although Time-Resolved Fluorescence can be performed with the monochromator, the
filter-based fluorescence system is more sensitive for TRF and is the better choice.
Models with Injectors
To keep the dispense system in top condition, flush and purge the fluid lines with
deionized (DI) water every day or upon completion of an assay run, whichever is more
frequent. Some reagents may crystallize or harden after use, clogging the fluid
passageways. Flushing the tubing at the end of each day, letting the DI water soak, and
then purging the lines at the beginning of each day ensures optimal performance of the
dispense system. See the Preventive Maintenance chapter for more information.
When dispensing volumes less than or equal to 20 µL/well, we recommend specifying a
tip prime volume that is equal to the dispense volume. For dispense volumes greater
than 20 µL/well, we recommend a tip prime volume of 20 µL.
To avoid spillage and possible contamination of the instrument, empty the tip prime
trough frequently and do not exceed the total fluid volume of the plate well when
dispensing.
Incubation and Partial Plates
When performing a partial plate read that includes an incubation step, the following
recommendations can reduce the effects of evaporation of your samples:
l
Use microplate lids.
l
Fill unused wells with liquid.
l
Cluster your sample wells rather than spacing them throughout the plate.
l
Place your sample wells in the center of the plate. This placement may lead to less
evaporation than if you place the samples in wells on the edge of the plate.
40 | Chapter 3: Getting Started
BioTek Instruments, Inc.