Optimize Performance | 101
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1 µL cassette = 5 seconds
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5 µL cassette = 7 seconds
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10 µL cassette = 10 seconds.
The MultiFlo FX will prime for this duration each time, which dispenses fluid
approximately equal to the dead volume for the cassette type.
Recommended prime volumes for the Syringe dispensers
The predefined maintenance protocols for the syringe dispensers fully prime the
system, pumping 40 mL through the tubing and pump:
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S-DAY_RINSE_A&B
- to fully prime both Syringe systems with 40 mL each
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S-DAY_RINSE_A or _B
- defined for each Syringe
Modify these protocols as needed when changing fluids and performing other
tasks. Edit the protocol to add another prime cycle when changing fluids to make
sure all previously used fluid is expelled and replaced with the new liquid.
Quick Prime
: 5000 µL is dispensed each time the quick prime is run.
The approximate dead volume for each Syringe dispenser system is 12 mL. Generally,
three times the dead volume completely primes the system. When using precious fluids,
e.g., expensive reagents, you can change the prime parameters: reduce the volume or
number of cycles specified in the predefined protocols or create your own protocols. Use
the priming trough inserts to capture expensive reagents when priming.
Optimize protocols to improve evacuation
When a wash protocol leaves too much residual fluid in the wells, optimize the
protocol with these recommendations:
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Add a secondary aspiration to a wash cycle below
, including
Final Aspirate
,
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Decrease the aspirate
Travel Rate
,
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Add a
Delay
to the Aspirate and/or Final Aspirate step ,
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Lower the aspirate height (Z-axis position).
Add a secondary aspiration to a wash cycle
When too much residual is left in the wells during a wash cycle, you can add a
secondary aspiration to reduce it. Secondary aspiration can be performed
BioTek Instruments, Inc.