Biotek Epoch 2, Instructions For Use Manual

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Getting Started Page 30 of 42
Epoch 2 Instructions for Use
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Revision
A BioTek Instruments, Inc.
Run a Take3 Session
Using the Touchscreen
The Epoch 2 is preprogrammed with seven Take3 protocols, available for use after a
Take3 plate has been defined and aligned on the reader.
The protocols measure at 260, 280, and 320 nm and cannot be edited. For the nucleic
acid protocols, a secondary wavelength of 230 nm can be selected at run time. For all
seven protocols, a spectral scan may also be selected.
Protocol name
Mass Extinction
coefficient
Secondary Ratio WL
(run time option)
Spectral Scan
(run time option)
dsDNA 50 230 nm 240 – 300 nm
RNA 40 230 nm 240 – 300 nm
ssDNA 33 230 nm 240 – 300 nm
1 Abs@1cm =
1 mg/mL
10 - 260 – 320 nm
BSA 6.7 - 260 – 320 nm
IgG 13.7 - 260 – 320 nm
Lysozyme 26.4 - 260 – 320 nm
Define and Align a Take3 Plate
Ensure that the glass slide is clean before running the alignment procedure.
Refer to the
Take3 / Take3 Trio User Guide for cleaning instructions.
If you remove or replace the glass slide, perform the Pathlength Calibration
procedure described in the
Take3 / Take3 Trio User Guide and then run the
alignment procedure described below. Update the Pathlength Values on the
touchscreen with the new values generated through the Pathlength Correction
procedure described in the user guide.
The reader can be configured with only one Take3 plate at a time.
This procedure ensures that the Take3 slide's microspot positions are captured
correctly in the reader's software. Upon completion, the Take3 tab appears on the
Main Menu and contains the preprogrammed Take3 protocols.
1. From the Main Menu , tap Instrument and select the Take3 tab.