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Biotage

®

SPE Dry 96 and

Biotage

®

SPE Dry 96 Dual

User Manual

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Summary of Contents for SPE Dry 96

Page 1: ...Biotage SPE Dry 96 and Biotage SPE Dry 96 Dual User Manual ...

Page 2: ...rranty and Liability 3 Installation 3 Connecting the Gas Supply 3 Connecting the Electrical Supply 4 Operation 4 Plate Clearance and Adjusting Plate 4 Adjusting Gas Flow Rate 4 Temperature Control 5 Method Development 5 Starting Guidelines 5 Thermally Labile Samples 5 Solvent Evaporation Times 5 Needle Depth 6 Maintenance 6 The System 6 Suggested Wash Procedure for the Needles 7 Needle Replacement...

Page 3: ...es collection plates are placed on the adjustable lower needle assembly and then raised to the upper needle assembly via the sliding arm The collection plate may be raised to any height or distance from the upper needle assembly allowing the user to develop methods utilizing a variety of plates and sample volumes Gas temperatures from both needle assemblies are fully user adjustable For operation ...

Page 4: ...t the upper head and ambient to 80 C at the lower head The temperature is determined by the operator s setting of the digital temperature controllers Do not attempt to use the system outside of these guidelines The system has a fully adjustable lower head that moves vertically to accommodate most plate depths and solvent volumes For large volume assays where many plates of the same size and volume...

Page 5: ... The system requires at least 25 L min of gas flow in order to operate the heating units These heaters are set to shut off automatically below 25 L min While the use of an in line filter moisture or particle helps avoid sample contamination they tend to be restrictive of the gas flow If you choose to use one be sure to use high flow rate filters Connecting the Electrical Supply Insert the power co...

Page 6: ...w before turning on the system power switch Adjust the gas flow to the desired rate for your method A good starting point for the flow rate of the upper needle assembly is 50 L min SPE Dry 96 or 80 L min SPE Dry 96 Dual and 25 L min for the lower needle assembly Optimum drying results can usually be obtained by maintaining the 25 L min flow rate for the lower needle assembly This allows the lower ...

Page 7: ... humidity Method Development Based on your needs and sample type you may adjust the parameters accordingly Since sample composition and volume are not usually negotiable you will probably be adjusting temperature and system gas flow to vary your methods If you are working with biological samples you may be further restricted by the fact that too much heat may alter your sample Thermally Labile Sam...

Page 8: ...les are 304 stainless steel and the head assembly is constructed of acetal copolymer It is highly recommended that an appropriate daily cleaning procedure be devised so that the needle assemblies do not become contaminated or attacked by harsh solvent There are a number of ways to clean the needle assemblies The two most common methods are submersion and sonication Submersion 1 Submerse the needle...

Page 9: ...the needle replacement tool 3 Position the needle perpendicular to the needle head assembly see Figure 10 Figure 10 Pounding unit Handle New needle Inserting the new needle into the needle assembly 4 Drive the needle into the needle assembly with the pounding unit until the housing of the needle replacement tool reaches the surface of the needle assembly completely 5 Remove the needle replacement ...

Page 10: ...e inlet gas supply pressure is set between 15 and 50 psi SPE Dyr 96 or 15 and 60 psi SPE Dry 96 Dual Check that the gas flow is set correctly Ensure that the needle assembly is seated and locked down properly Check for leaks at the hose inlet and in the supply hose Visually inspect needle assemblies to ensure they are not plugged Certain wells do not dry down Visually inspect that the needles are ...

Page 11: ...PE Dry 96 Dual 10 0 x 15 5 x 15 0 254 mm x 394 mm x 381 mm Weight SPE Dry 96 25 lbs 11 36 kg SPE Dry 96 Dual 31 lbs 13 9 kg Inlet Gas Connection 1 4 NPT Inlet Gas Pressure Minimum 15 psi maximum 65 psi Minimum Operating Flow Rate 25 L min Maximum Operating Flow Rate SPE Dry 96 60 L min SPE Dry 96 Dual 90 L min Maximum Operating Gas Temperature 60 C upper head 80 C lower head Specifications ...

Page 12: ...B 2017 Biotage All rights reserved No material may be reproduced or published without the written permission of Biotage Information in this document is subject to change without notice and does not represent any commitment from Biotage E OE A list of all trademarks owned by Biotage AB is available at www biotage com legal Other product and company names mentioned herein may be trademarks or regist...

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