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© 2015 biostep GmbH , V1.3 - 09.2015
4.2.1.4
Histogram
Im g. 18 Histogram
1
M
ax. peak height
2
M
in. grey scale value
3
M
ax. grey scale value
4
Grey value area that is to be stretched.
All grey values outside of this area are not
used for the calculation of the image
display. Due to the limitation of this area,
a clear contrast enhancement arises,
especially with samples lighting very
weakly.
5
Automatic image optimisation.
Sets lower and upper grey scale limits with the aid of calculation values set in the
settings. Use this function to quickly adjust a good contrast ratio.
6
Full dynamic range sets lower limit to minimum (
2
) and upper limit to maximum (
3
).
7
Sliding controller for the manual adjustment of the grey scale limits.
8
Direct input field for lower grey scale limit.
9
Increment by which the value is to be increased or to be reduced.
q
Direct input field for upper grey scale limit.
4.2.1.5
Automatic exposure time calculation
Im g. 19 Autom atic exposure tim e calculation
1
Start of the automatic suggestion
calculation
2
to
5
calculated suggestions.
Clicking on one of these buttons
automatically sets the respective
exposure time and the respective binning.
Afterwards, one click on the button is
sufficient for "Capture".
The sensitivity of the automatic suggestion calculation can be modified in the
settings.
4.2.2
What is binning?
At binning, the brightness values of adjacent pixels are added and assigned to one individual
pixel. For example, binning 3 x 3 means that the brightness values of 3 x 3 = 9 pixels are
added and assigned to one pixel. This increases the sensitivity, but reduces the resolution.