Product Manual
www.bioline.com/isolate
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2. Description
ISOLATE Plant RNA Mini Kit is specially designed for the fast and efficient isolation of
extremely pure total RNA from a variety of plant tissue samples. The isolated RNA is
ready for downstream applications such as reverse transcription, real-time PCR and
RNA protection assays.
The protocol is easy to follow on a step-by-step basis. Two lysis buffers are provided.
In most cases, Lysis Buffer APR is recommended. In cases where yield is poor with
Lysis Buffer APR, Lysis Buffer BPR should be used. Once the cells are lysed, the
lysate is applied to a Spin Column to selectively remove genomic DNA. There is no
need to perform a separate DNase digestion step. The lysis buffer is optimized to
efficiently lyse the cells by incubation in a chaotropic lysis buffer. The lysis buffer also
inactivates RNases, thus protecting the released RNA. The RNA is then bound to a
silica membrane. Subsequent wash steps remove the remaining cell debris. Pure RNA
is eluted in the final step with RNase-free water.
3. storage
The ISOLATE Plant RNA Mini Kit should be stored dry at room temperature. Under
these conditions, the kit is stable for 12 months.
4. safety information
Always wear gloves and a suitable lab coat when handling the reagents of this kit. For
detailed information, refer to the material data safety sheets (MSDSs) available on our
website at www.bioline.com.
5. proDuct specifications
starting material
Plant tissue (up to 100mg)
time required
30 minutes after homogenization
features
•
Rapid protocol: 30 minutes after
homogenization
•
High purity RNA
•
Clear, easy to follow instructions
Binding capacity
Approximately 100µg RNA
applications
Isolation of RNA from:
•
Fresh or frozen plant material
