GeneExplorer Thermal Cycler Instruction for Use
Chapter One Introduction
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Chapter One Introduction
The chapter covers introduction to PCR technology and examples of its applications,
introduction to PCR Thermal Cycler and features of GeneExplorer Thermal Cycler.
1
Introduction to PCR technology
Polymerase Chain Reaction (PCR in short) is a nucleic acid amplification technology of in-vitro
simulation of natural DNA reproduction process. With two DNA chains to be amplified as a
template and mediated by a pair of artificially synthetic ODN primers, and through DNA
polymerase of high temperature resistance, it can rapidly and specifically amplify specific DNA
segment. Due to its simplicity, rapidity, peculiarity and sensitivity, ever since invention of PCR
by Mullis in 1985 and discovery of DNA polymerase of high temperature resistance by Erlich in
1988, over a few years, the technology has been widely applied in various fields of bioscience.
Especially in cytology, virology, oncology, genetic epidemiology, forensic pathology and flora
and fauna immunology, it has accomplished outstanding achievements and become a milestone
of contemporary molecular biology development.
2
Examples of PCR’s applications
Application of Thermal Cycler: The product is applicable to polymerase chain reaction in fields
including immunology, human genome engineering, forensic pathology, oncology, organization
and population biology, paleontology, zoology and botany.
3
Intended use
The GeneExplorer Thermal Cycler is an automated instrument used for qualitative amplify the
related nucleic acids in human sample using the polymerase chain reaction process. The
instrument is for in vitro diagnostic only.
The GeneExplorer Thermal Cycler is intended for used in medical and biological laboratories by
professional user trained in molecular biological techniques and the operation of GeneExplorer
Thermal Cycler.
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Features of GeneExplorer Thermal Cycler
GeneExplorer Thermal Cycler is a PCR of multiple functions and applications. It features as
below:
⚫
Providing up to eight replaceable modules based on requirements of various PCR
experiments.
