5.4 Attach the Reservoir:
There is a 60 cc syringe in the accessory kit. Pull the plunger out and connect it to the luer-loc
connector at the end of the blue tubing on the right side of the box (pull the tubing out first). Slide the
flanges on the top of the syringe barrel under the screw heads on either side of the notch in the right
wing. Fill the reservoir with
FILTERED
water or buffer, depending on your needs. Small particles of
dust and debris can clog the valve in the piston tip. The salt in buffer will reduce the carryover between
fractions and keep your samples isotonic. However, it will also put more load on your scintillation
cocktail if you are counting your rinses.
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