7
3.
Click ‘Quick Measurement’:
4.
The microplate reader is now ready for use.
3.3 Quick Measurement:
Performing a Quick Measurement on Galapagos:
1.
Click the ‘Quick Measurement’ icon on the main menu screen:
2.
Select the appropriate measurement mode (Single, Dual or Multi-wavelength):
a.
b.
Single wavelength
refers to absorbance measurement at one wavelength.
c.
Dual wavelength
refers to the measurement of a sample at two wavelengths. The first
wavelength is where the sample of interest absorbs. The second wavelength is used as
a reference and will be subtracted from the first wavelength. The absorbance
measurement is then subtracted from a measurement at a different wavelength.
Multiple wavelength
refers to a series of absorbance measurements at different
wavelengths. In this mode, absorbance measurements are not subtracted from one
another.
d.
Wavelength scan
refers to absorbance measurements over a specified wavelength
range (340-800nm).
