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EZ Read 2000 Microplate Reader

User Manual

Biochrom Ltd

22 Cambridge Science Park

Cambridge

UK

CB4 0FJ

Tel.: +44/1223 423723

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Summary of Contents for EZ Read 2000

Page 1: ...1 EZ Read 2000 Microplate Reader User Manual Biochrom Ltd 22 Cambridge Science Park Cambridge UK CB4 0FJ Tel 44 1223 423723...

Page 2: ...CONNECTION 6 3 2 SOFTWARE INSTALLATION 6 3 3 QUICK MEASUREMENT 7 3 4 KINETIC MEASUREMENT 12 3 5 MULTI WAVELENGTH MEASUREMENTS 16 3 6 WAVESCAN MEASUREMENTS 20 3 7 CALIBRATING THE INSTRUMENT 23 4 TECHNI...

Page 3: ...ONS 25 7 2 RETURNS TERMS AND CONDITIONS 26 7 3 RETURNS PROCEDURE 26 8 ORDERING INFORMATION AND ACCESSORIES 28 9 CONTACT INFORMATION 28 9 1 TABLE 2 CONTACT INFORMATION 28 10 APPENDIX 29 10 1 HELPFUL HI...

Page 4: ...ion What is a microplate reader A microplate reader is a used to measure the absorbance of liquid samples in a 96 welll plate What do microplate readers measure Microplate readers measure the amount o...

Page 5: ...ance based microplate readers are ELISA total protein like Bradford or BCA and cell proliferation assays 2 Instrument Use The Biochrom EZ Read 2000 Microplate Reader is used for microplate based appli...

Page 6: ...supplied USB cable 2 To connect the instrument to Galapagos software Insert the CD or USB supplied with the instrument into a PC Galapagos software includes pre requisite files dot NET Framework 4 Wi...

Page 7: ...refers to the measurement of a sample at two wavelengths The first wavelength is where the sample of interest absorbs The second wavelength is used as a reference and will be subtracted from the first...

Page 8: ...lect to shake the microplate Users can select shaking to be a single event or in timed cycles 4 Select the amplitude degree of linear shaking 5 Select the speed for linear shaking 6 Users can select t...

Page 9: ...ick Start to begin acquisition 8 Raw absorbance values for dual wavelengths measurements are displayed in the wells Details of corresponding wavelengths can be displayed when clicking on the individua...

Page 10: ...values are displayed The bold value refers to the absorbance measurement after wavelength subtraction of the absorbance at the measurement wavelength from the absorbance at the reference wavelength Th...

Page 11: ...ternal database system such as LIMS Users can save files and templates onto the database File save to own personal file or Save Method Template saves the method protocol for future use 11 Results can...

Page 12: ...measurements click on the timer icon for timed measurements This is indicated by Timing On Users can then input the required kinetic parameters for a measurement cycle The measurement cycle refers to...

Page 13: ...gle Dual or Multiple wavelength modes Note measuring at more than one wavelength will affect the interval that can be used The interval time must take into account the measurement time at a single wav...

Page 14: ...lect to shake the microplate Users can select shaking to be a single event or in timed cycles 7 Select the amplitude degree of linear shaking 8 Select the speed for linear shaking 9 Users can select t...

Page 15: ...layed showing both readings and cycle status at the measured wavelengths 12 Results are displayed in each well If dual wavelength measurements are carried out absorbance measurements at both wavelengt...

Page 16: ...a specific colour and identified by a legend 13 Results can be saved to Database A database is useful for saving sharing and extracting data via communication with an external database system such as...

Page 17: ...different wavelengths Mathematical manipulations of this data can often reveal information about the sample s composition or purity 1 To perform a multi wavelength measurements click Multiple Wavelen...

Page 18: ...the range of 340 to 800nm Click Start to begin the measurement 3 Individual multi wavelength measurements are displayed in each well with raw absorbance displayed on the right hand panel as a graph of...

Page 19: ...ful for saving sharing and extracting data via communication with an external database system such as LIMS Users can save files and templates onto the database File save to own personal file or Save M...

Page 20: ...gth range This is important in terms of sample purity and detecting absorbance peaks at different wavelengths 1 To perform a wavescan measurement click Wavescan from the drop down menu 6 Results can a...

Page 21: ...rs can also click on Timing to enter timed intervals between scans Click Start to begin the measurement 3 Individual wavescans for each well are shown on the page Click on an individual well to attain...

Page 22: ...se system such as LIMS Users can save files and templates onto the database File save to own personal file or Save Method Template saves the method protocol for future use 6 Results can also be may be...

Page 23: ...5 1 Measurement modes Single dual and multi wavelength and kinetics measurements with absorbance data output in OD on display 5 2 Integrated quality control Post sample filtering to eliminate ambient...

Page 24: ...n and disinfected Only authorized and trained personnel in a well ventilated room while wearing disposable gloves protective glasses and clothing should perform the cleaning and disinfection procedure...

Page 25: ...y period of 24 months shall be granted to the original buyer of the Biochrom EZ Read 2000 Microplate Reader The warranty shall lose effect if any of the below conditions occur Biochrom EZ Read 2000 Mi...

Page 26: ...able for 7 years after the instrument is no longer manufactured 7 2 Returns terms and conditions In the event that you need to return your instrument to us for repair or to use our in house recalibrat...

Page 27: ...mport Item Returning to Supplier for Repair 4 Send the package to Biochrom using the address label found on the Biochrom website http www biochrom co uk content 1 65 returns html and your own carrier...

Page 28: ...Check Plate 9 Contact Information Table 2 Contact Information Department Email contact Telephone contact Sales enquiries biochrom co uk 44 0 1223 423723 Technical Support support biochrom co uk 44 0 1...

Page 29: ...which your molecule of interest does not absorb Many assays recommend a reference measurement at 620 nm When using plate frames for stripwells or single wells breakable strips check that all strips a...

Page 30: ...certain controls such as positive or negative controls Users can input details of the controls such as the name and type of control Users can use threshold calculations to subtract readings from other...

Page 31: ...y used reference wavelength in many absorbance applications in the visible range Check the product data sheet for recommended reference wavelength for your specific assay 6 How can I connect the instr...

Page 32: ...1 2010 Safety requirements for electrical equipment for measurement control and laboratory use General requirements EN61010 2 101 2002 Safety requirements for electrical equipment for measurement con...

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