Bio-Tek ELx800, Operator'S Manual

Page: 149 / 214

Liquid Testing
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121
Liquid Test 2
The recommended method of testing the instrument performance is to use
the Absorbance Test Plate to confirm alignment, repeatability, and
accuracy, which will also confirm linearity.
If a Test Plate is not available, Liquid Test 2 can be used for these tests.
Required Materials:
• New 96-well, flat-bottom microplates (Corning Costar® #3590 is
recommended)
•
Ten test tubes, numbered consecutively, stored in a rack
•
Calibrated hand pipette (Class A volumetric pipette
recommended)
• Stock solution A or B (these are the same solutions as for Liquid
Test 1)
Preparation of Dilutions:
1 Set up a rack containing 10 tubes, numbered consecutively.
2 If you have not already done so, prepare concentrated stock Test
Solution A or B.
Refer to Table 3 when executing steps 3 and 4.
Table 3
Test Tube Dilutions
Tube Number 1 2 3 4 5 6 7 8 9 10
Volume of Concentrated
Solution (ml) 20 18 16 14 12 10 8 6 4 2
Volume of 0.05% Tween
Solution (ml) 0 2 4 6 8 10 12 14 16 18
Absorbance expected if
concentrated solution is
2.0 at 200
µ l
2.0 1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2
3 Create a percentage dilution series, beginning with 100% of the
concentrated stock solution in tube 1, 90% of the concentrated
solution in tube 2, 80% in tube 3, and so on to 10% in tube 10.
4
Dilute using amounts of the remaining 0.05% solution of
deionized water and Tween 20, as shown in
Table 3 .
ELx800 Operator's Manual