BIO RAD Helios Gene Gun System, Instruction Manual

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Section 9 Product Information
Helios Gene Gun System | 41
Problem Poor or no expression.
Possible solutions Make sure DNA is resuspended in TE or distilled water, not saline.
Check that DNA has been precipitated onto the microcarriers
(see Quantification of DNA in Cartridges, in Section 10).
Problem Attached cells are lifting off the center of the plate after delivery of gold particles,
or there is a dead zone in the center of the target.
Possible solutions Lower the helium pressure and/or lower the microcarrier loading rate.
Problem Contamination of the cell culture.
Possible solution Wipe the end of the barrel with ethanol or dilute bleach.
Section 9
Product Information
Helios Gene Gun System
The catalog numbers for the two Helios Gene Gun Systems (100/120 V and 220/240 V) and component
descriptions are as follows:
Catalog # Description
1652431 Helios Gene Gun System, 100/120 V includes
1652411 Helios Gene Gun Kit, that includes Gene Gun Unit, 1
1652416 O-rings, Helios Gene Gun, 5
1652417 Barrel Liner, 5
1652426 Cartridge Holder, white, 5
1652435 Cartridge Extractor Tool, 1
1652436 Battery, 9 V, 1
1652412 Helium Hose Assembly, with Swagelock Quick-Connect Fittings, 1
1652413 Helium Regulator, CGA 580 female fitting, with pressure relief
valve. Maximum pressure 2,600 psi., 1
1652418 Tubing Prep Station, includes Tubing Prep Station, 100/120 V, 1 tubing support cylinder, 1 power cord, O-rings, 5 Tubing Prep
Units (1652419), 12 ft nitrogen regulator hose (Nalgene), two 3/16” barb to male luer fittings, nitrogen flowmeter fitting, two 1/8”
barb-to-male luer fittings, one 5/64" Allen wrench, one 10cc syringe holder
165-2421 Macro-Prep DEAE Resin , 500 ml
165-2422 Macro-Prep DEAE Resin , 5 L
165-2424 Macro-Prep DEAE Resin , 10 L
1652432 Macro-Prep CM Resin , 25 ml
7326100 Quantum Prep Plasmid Miniprep Kit, 100 preps
7326120 Quantum Prep Plasmid Miniprep Kit, 20 preps