CFX96 and CFX384 Systems Manual
23
Figure 24 shows the default end point protocol.
Figure 24. End Point Only protocol.
To change the step temperature or sample volume for the end point only run, click the
Start
Run
tab and edit the
Step Temperature
or
Sample Volume
. Figure 25 shows the step
temperature is changed to 55.0oC.
Figure 25. Change the sample volume on the Start Run tab.
Plate Tab
The Plate tab shows a preview of the selected plate file loaded in the Experiment Setup
(Figure 26). In a real-time PCR experiment, the plate file contains a description of the contents
of each well, the scan mode, and the plate type. CFX Manager software uses these
descriptions for data collection and analysis.
Select one of the following options to select an existing plate, create a new plate, or edit the
currently selected plate:
•
Create New button.
Open the Plate Editor to create a new plate
•
Select Existing button.
Open a browser window to select and load an existing plate file
(.pltd extension) into the Plate tab
•
Express Load pull-down menu.
Quickly select a plate to load it into the Plate tab
TIP: To add or delete plates in the
Express Load
menu, add or delete files (.pltd
extension) in the
ExpressLoad
folder. To locate this folder, select
Tools > User
Data Folder
in the menu bar of the main software window.
Summary of Contents for CFX384
Page 9: ...Table of Contents viii ...
Page 49: ...Protocols 40 ...
Page 89: ...Data Analysis Overview 80 ...
Page 149: ...Resources 140 ...
Page 157: ...Index 148 ...
