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BIO RAD Bio-Plex Pro, Instructions For Use Manual

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BIO RAD Bio-Plex Pro Instructions For Use Manual Download Page 74

Troubleshooting and Error Messages 

74                                                                                                                  Bio-Plex Pro and Bio-Plex Pro II Wash Stations 

 

 

 

WARNING 

Depending on the applications, parts of the Instrument may have been in 
contact with biohazardous material. 

•

 

Make sure to treat this material according to the applicable safety 

standards and regulations. 

•

 

Always clean and disinfect all parts before disposal. 

 

Dispose of packaging material according to local regulations. 

Summary of Contents for Bio-Plex Pro

Page 1: ...Instructions for Use for Bio Plex Pro Bio Plex Pro II Wash Stations Revision B Attention Use only the magnetic carrier which arrived with your instrument See page 17 for more details...

Page 2: ...ii Bio Plex Pro and Bio Plex Pro II Wash Stations...

Page 3: ...performing hands free wash programs on Bio Plex and other xMAP assays It contains instruction for the use of the instrument and is intended as a reference for the user It contains information about th...

Page 4: ...iv Bio Plex Pro and Bio Plex Pro II Wash Stations...

Page 5: ...ng and Installing a Plate Carrier 17 Changing the Vacuum Configuration 18 INSTALLING THE MICROPLATE 19 TURNING ON THE POWER 19 KEYPAD BASICS 20 Primary Menu 20 Secondary Menu 20 PRIMING THE INSTRUMENT...

Page 6: ...OMPLETE INSTRUMENT KEYPAD FUNCTIONS 43 Alphabetical list of LCD text with explanations 45 DEFINING CUSTOM WASH PROGRAMS 48 WASH MODES 48 Washing Positions 49 ASPIRATING MODES 50 Normal AspirationMode...

Page 7: ...ESSAGES 75 ERRORS WITHOUT MESSAGES 75 ERROR MESSAGES 75 Plate Error 75 Program Too Big Error 75 Transport Error 75 Manifold Arm Error 76 Sensor Defect Error 76 RS485 Error 76 PC Process Control System...

Page 8: ...ates the possible presence of biologically hazardous material Proper laboratory safety precautions must be observed Attention Negative environmental impacts associated with the treatment of waste z Do...

Page 9: ...nal Electrotechnical Commission IFU Instructions for Use in Inch IVD In Vitro Diagnostics IVD D In Vitro Diagnostics Directive kg Kilogram l L Liter m Meter mBar Millibar ml Milliliter mm Millimeter m...

Page 10: ...Basic Operation and Maintenance 10 Bio Plex Pro and Bio Plex Pro II Wash Stations...

Page 11: ...nd other xMAP assays The wash station is available in two versions Wash Configuration Bio Plex Pro Bio Plex Pro II Vacuum filtration No Yes Magnetic separation Yes Yes Both wash stations are delivered...

Page 12: ...pport Only these recommended filter plates should be used with the Bio Plex Pro II when configured for standard Bio Plex or other polystyrene bead based xMAP assays Recommended microplates for vacuum...

Page 13: ...no possibility of condensation causing a short circuit 1 Ensure that the on off switch in the rear panel of the instrument is in the off position 2 Connect the solution tubes L1 and L2 to the inlet co...

Page 14: ...Basic Operation and Maintenance 14 Bio Plex Pro and Bio Plex Pro II Wash Stations Bio Plex Pro Installation Diagram...

Page 15: ...Basic Operation and Maintenance Bio Plex Pro and Bio Plex Pro II Wash Stations 15 Bio Plex Pro II Installation Diagram...

Page 16: ...II only Your Bio Plex Pro II is shipped with the liquid hoses attached Attach the tubing bracket to the left side of the instrument as shown in the pictures below Location of Tubing Bracket A tool is...

Page 17: ...d the front of the instrument until it clears the guide bar Prime Tray Guide Bar Installing the plate carrier is the reverse of the procedure above 1 Carefully guide the plate carrier onto the guide b...

Page 18: ...e of Enable The instrument must be turned off and then back on for the change to take effect OPTIONS EXIT OK EDIT LLD CHANNEL EXIT OK Select and press OK for OPTIONS System places you in the Options m...

Page 19: ...ectly oriented position A1 of the plate corresponds to position A1 marked on the plate transport If using the vacuum plate carrier for polystyrene based assays see Changing the Plate Carrier on page 2...

Page 20: ...ndary Menu If you choose OK when any of the primary menu options Programs Settings and Procedures are visible on the LED you will navigate to the secondary menus You may Select a choice from the secon...

Page 21: ...as described above Alternately you may carefully use a syringe to inject 1 2 ml of water directly into the L1 port of the instrument reconnect the tubing and run a prime procedure PRIME OK CHANNEL 1...

Page 22: ...are found in the Defining New Programs section on page 53 START OK SSSSSSSSSSSS OK RUN1 Program X EXIT OK PROGRAM OK Navigate to PROGRAM in the primary menu and press OK Use the keys under the and sym...

Page 23: ...irst wash magnetic assay MAG x2 All other washes magnetic assay MAG x3 Prewash vacuum assay PREVAC First wash vacuum assay VAC x2 All other washes vacuum assay VAC x3 Third party kits which require 20...

Page 24: ...pressure to the plate for the first vacuum cycle of the day Monitor your plate periodically to ensure complete removal of wash buffer before the next dispense cycle Blot the bottom of the filter plat...

Page 25: ...isplayed RINSE DAY STOP END The manifold remains in the prime tray until END is pressed Press STOP to abort the procedure prime tray will not be aspirated Navigate to PROCEDURE in the primary menu and...

Page 26: ...instrument on if you want to soak the manifold overnight Recommended during periods of heavy use Shut the instrument off if you do not wish to soak the manifold Rinse Night only Navigate to PROCEDURE...

Page 27: ...ystem as outlined above When the rinsing procedure is completed the following message is displayed RINSE NIGHT STOP END The manifold remains in the prime tray until END is pressed Press STOP to abort...

Page 28: ...id system if necessary Perform Rinse Day with wash buffer if the instrument is left to stand for a short time up to 2 hours Use distilled water if you anticipate longer intervals between uses Perform...

Page 29: ...and clean each instrument needle by inserting the cleaning needle into the instrument needle and gently agitating to remove any possible debris Then rinse with water If you choose to remove the manifo...

Page 30: ...are not answered in Basic Operation and Maintenance The reference section is provided for customers interested in more detail about the operation of the wash stations Provided you follow standard saf...

Page 31: ...ty precautions such as wearing protective clothing and using approved laboratory safety procedures Note Suitable Compact Microplates The instrument supports the processing of flat round or v shaped bo...

Page 32: ...ode 21640582 the recommended concentration is 1 ml of antifoaming agent for 1 liter of waste solution Use antifoaming agents and concentrations as recommended by corresponding manufacturers Intended U...

Page 33: ...43 Use the appropriate standard programs Both instruments can also be used to process other plate based assays If you wish to do so you can leave the magnetic carrier in place If you have concerns abo...

Page 34: ...hannel manifold USB Interface All connected devices must be approved and listed as per IEC 60950 1 Information Technology Equipment Safety or equivalent local standards Variable Volume of solution dis...

Page 35: ...20 240 V 50 60 Hz Consumption 65 VA Physical Outside dimensions without Vacuum Filtration Width 275 mm Depth 366 mm Height 180 5 mm Width 10 8 in Depth 14 4 in Height 7 1 in Greater depth and height i...

Page 36: ...ro and Bio Plex Pro II Wash Stations Description The diagram below shows the main components of the instrument Keyboard and Display Unit Manifold Arm Manifold Prime Tray Plate Support Guide Bar Power...

Page 37: ...About the Instrument Bio Plex Pro and Bio Plex Pro II Wash Stations 37 Bio Plex Pro Dimensions...

Page 38: ...About the Instrument 38 Bio Plex Pro and Bio Plex Pro II Wash Stations Bio Plex Pro II Dimensions...

Page 39: ...late is located under the main power socket The company information on the top differs depending upon where it is purchased The nameplate also shows the model name the model part number and the serial...

Page 40: ...hey should not be placed more than 1 m below the level of the instrument since this may result in instrument malfunction For information regarding outer dimensions and weight of the instrument see the...

Page 41: ...h lid 6 Bundle of 2 tubes for connecting to the liquid bottles 5mm outer diameter 3mm inner diameter 7 Main power cable 8 Instrument manual 9 CD with software and support material 10 10 Bio Plex Pro f...

Page 42: ...message is displayed If a Rinse procedure was aborted before the instrument was switched off the following message is displayed after the instrument initializes Press OK and then select a Rinse proced...

Page 43: ...m each processing step can be identical or different from the previous step Adjustable soak time 1 second to 60 minutes and 59 seconds Variable shaking settings o High Linear shaking with a shaking fr...

Page 44: ......

Page 45: ...Defining New Programs Bio Plex Pro and Bio Plex Pro II Wash Stations 45 Alphabetical list of LCD text with explanations...

Page 46: ...Defining New Programs 46 Bio Plex Pro and Bio Plex Pro II Wash Stations...

Page 47: ...Defining New Programs Bio Plex Pro and Bio Plex Pro II Wash Stations 47...

Page 48: ...ted in the program otherwise spilling can occur and the instrument may become contaminated WARNING When the instrument is busy do not touch the manifold After the instrument has been used the manifold...

Page 49: ...ow and bottom position Move Overflow If Move Overflow MOVE OVER is selected the manifold moves step wise from the bottom position to the Overflow position during dispensing It is recommended for cell...

Page 50: ...d bottom or V shaped bottom wells the aspirating needles are placed in the middle of the wells Only one aspiration position can be selected Round Bottom Wells V Shaped Wells Normal Aspirating Position...

Page 51: ...e detachment of cells and increases the remaining cell percentage at the bottom of the wells The following example shows a typical wash program for working with adherent cells as it would appear in th...

Page 52: ...to the next parameter Select PROGRAM END and press OK Set the volume to 450 l and press OK to continue to the next parameter set the channel to 1 and press OK to continue to the next parameter Set th...

Page 53: ...ciency SOAK During a soak step the liquid remains in the wells for the set time with or without shaking VAC For collecting desired substances by vacuum filtration using filtration plates USER PROMPT U...

Page 54: ...repeated up to 9 times C1 P1 ASP EXIT OK Press or to select the process step ASP DISP WASH SOAK PROGRAM END CX CYCLE where X is the number of the next cycle Press OK to define the selected process ste...

Page 55: ...spiration or select NO to perform normal aspiration Press or to select the aspiration position BOTTOM OVERFLOW or CUSTOM Select required aspirating rate 1 3 where 1 is the weakest and 3 the strongest...

Page 56: ...Select the volume of solution to be used 50 400 l in increments of 50 l Select and confirm dispensing step DISP CX Cycle Number PX Program Step Number CX PX DISP EXIT OK VOLUME 300 l EXIT OK CHANNEL...

Page 57: ...ispensing Position Diagrams Z Position Bottom Z Position Custom Z Position Overflow Position Diagrams MOVE OVER Overflow Position Movement of manifold MOVE CUST Custom Position Movement of manifold Bo...

Page 58: ...of solution to be used 50 3000 l in increments of 50 l Select and confirm wash step WASH CX Cycle Number PX Program Step Number Select required flow rate drip to 500 l sec Select required aspirating...

Page 59: ...select the next program step Program End or a new cycle Process Step User Prompt Select and confirm User Prompt strip on required position in current program CX PX USER PR EXIT OK Process Step Vacuum...

Page 60: ...o return to the previous step Cycle 1 will be shown Press to show the program steps of this cycle Press OK to show the cycle number POS OVERFLOW EXIT OK VOLUME 300 l EXIT OK CHANNEL 1 EXIT OK H SPEED...

Page 61: ...d the following message is displayed PROGRAM IS LOCKED The program can only be unlocked via the HydroControl software by users with the appropriate rights Settings Menu The Settings menu has the follo...

Page 62: ...spiration can be defined The instrument moves to the predefined position Use the and keys to change the position Confirm with OK The next position can then be selected Press OK to save the defined pos...

Page 63: ...edures Prime Start prime procedures Empty Prime Tray Aspirate liquid out of the prime tray Vacuum Filtration Prepare the vacuum pressure to prevent delays during the running of a wash program The Proc...

Page 64: ......

Page 65: ...instrument is to rinse the liquid system with distilled water before the instrument is left to stand or switched off at the end of each day The manifold should be removed and cleaned thoroughly at le...

Page 66: ...be removed from the instrument Autoclaving max 130 C max five times manifold must be removed from the instrument Do not autoclave manifolds with the Process Control option Reinstall the manifold if ne...

Page 67: ...quid or foam may occur when the Instrument is operated improperly such as Microplate used does not match installed manifold The strip plate positions do not match the positions defined in the program...

Page 68: ...ifold WARNING After the instrument has been used the manifold may be infectious Before the manifold is removed it must be thoroughly disinfected It is advisable to adhere to applicable safety precauti...

Page 69: ...at least once every 6 months or whenever needles become blocked 1 Lift the manifold arm and remove the black manifold arm guard plate by removing the three screws using the Allen key provided 2 Lift t...

Page 70: ...es in the bracket 3 Tighten the manifold into place using the two screws using the Allen key provided 4 Fit the dispensing tubes marked blue onto the top connector on the rear of the manifold 5 Fit th...

Page 71: ...t the instrument is thoroughly disinfected before it is removed from the laboratory or any servicing is performed on it Before the instrument is returned to the service center for service or repair it...

Page 72: ...ing procedure should be used to disinfect the instrument 1 Prepare an autoclave bag for all disposables used during the disinfection procedure label it with autoclave tape and disinfect the material u...

Page 73: ...e treatment of electrical and electronic equipment waste Do not treat electrical and electronic equipment as unsorted municipal waste Collect waste from electrical and electronic equipment separately...

Page 74: ...on the applications parts of the Instrument may have been in contact with biohazardous material Make sure to treat this material according to the applicable safety standards and regulations Always cle...

Page 75: ...s Plate Error If the plate sensor does not recognize the plate or no plate is inserted on the plate carrier the following message is displayed NO PLATE OK Press OK and insert the plate correctly on th...

Page 76: ...that particular type of microplate Sensor Defect Error The following message error is displayed when the dispensing pump or the dispensing sensor is defective SENSOR DEFECT OK Press OK to remove the e...

Page 77: ...Your system is not equipped for process control so it must be deactivated The following directions describe how to check and disable a PC setting Remember to exit to the Primary Menu before performing...

Page 78: ...ur system This is an advanced function for which your system is not equipped All channels should be deactivated for LLD The following directions describe how to check and clear all channels of an LLD...

Page 79: ...Bio Plex Pro and Bio Plex Pro II Wash Stations 79 Declaration of Conformity...

Page 80: ...ad 20 Maintenance 28 Menus and the keypad 20 Navigating 20 Options 62 Overview 20 Primary 20 Procedures 63 Program 53 Quick Guide 24 Secondary 20 Settings 61 Microplates Installation 19 Recommended 12...

Page 81: ...Bio Plex Pro and Bio Plex Pro II Wash Stations 81...

Page 82: ...82 Bio Plex Pro and Bio Plex Pro II Wash Stations...

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