BIO RAD 145-1001, Instruction Manual

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22 | S3 Cell Sorter Instruction Manual 22 |
Fig. 17. Administrator tab toolbar.
Table 20. Instrument controls and their functions (continued).
Button Name Function
Fluidics System Indicates the status of the fluidics system and run time information. When
Status the fluidics system is down to an hour of run time, the fluidics status
window will automatically open to show which container needs attention.
If the run time reaches 5 min, the fluidics will automatically shut down
Toggle 488 nm Controls the 488 nm laser shutter. The shutter is interlocked for safety. When
Laser Shutter this button is grayed out, either the laser is off or the interlock is open
Toggle 561 or 640 nm Controls the 561 or 640 nm laser shutter. The shutter is interlocked for safety.
Laser Shutter When this button is grayed out, either the laser is off or the interlock is open
Adjust Laser The dropdown arrows next to each laser adjust the laser power. Actual
mWatt Power power is shown by hovering over the 488 nm, 561 nm, and 640 nm labels
Table 21. PMT controls and their functions.
Button Name Function
Editable The name for each parameter is editable and will be saved with the
parameter acquired FCS data. The edited parameter name will be applied to all
name plot axes, compensation controls, and filter configurations
PMT Voltage Adjusts the PMT voltage for the given detector
Select trigger The trigger parameter is used to alert the system to the presence of
parameter an event over the threshold. The default for this is Forward Scatter
Adjust threshold Threshold is a percentage of the trigger signal. The default value is 1.0%
setting
3.3 Administrator Tab Toolbar
The administrator tab is available only for administrator users. To change a user’s privileges,
refer to Section 3.3.5, Managing Users.
The toolbar for the administrator tab (Figure 17) consists of:
n
System and User Control — edit system controls and manage users
Table 22 describes the buttons in this section.
IMPORTANT! Adjusting any of these settings will impact the overall performance of
the system. Only knowledgeable users should make any changes to these settings.
All changes made here will apply globally to the system.