Beckman Coulter Navios, Instructions For Use Manual, Page: 65 / 586

773232AH
3-11
Operation Principles
Automated Software Features
3
causing Published data to be formatted as a single row of values. In the case that the Publish Data to
MS Excel option is selected, this progressively builds a table on a single spreadsheet.
Cleanse Protocols: Any Protocol name beginning with the word “Cleanse” overrides the Output
Options setting for Save LMD on the
Workspace Preferences - Acquisition Options tab. This allows
you to create protocols that do not write a listmode file to disk on completion.
AS Protocols: There is a series of special protocols which include “AS” in their names. These are
components of the AutoSetup applications which operate with the Auto Setup Wizard to set up
Volts, Gains and Compensation.
Special Panels
The cleanse panel utilizes two cleanse protocols (‘cleanse bleach.PRO’ and ‘cleanse di water.PRO)
that you should use at the end of the working day or shift on your Navios flow cytometer.
Automated Software Features
Navios software contains the following automated software features.
•
AutoSetup Wizard
•
QuickCOMP
•
QuickSET
•
Automatic Gate Creation
•
Automatic Gate Maintenance
•
Automatic Color Precedence
•
Elliptical and Contour AutoGating
•
AutoMATOR Analysis
Parameters
TIME Parameter
The TIME parameter is the amount of time, in seconds, the instrument acquires data. It is displayed
on the plot axis in 1-second resolution. The axis labels vary, depending on plot resolution and stop
time (duration).
NOTE
Including TIME as a selected parameter in all protocols allows for an internal quality check on the data
acquisition. A TIME versus fluorescence plot may be helpful to monitor system fluidic and optic conditions
during acquisition of any given sample. Monitor consistent fluorescence over time as shown below.
Unexpected fluctuations in the pattern of fluorescence may indicate compromised fluidics or optic
conditions.