Beckman Coulter Navios, Instructions For Use Manual

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Using Navios Software
Analysis Menu
Regions Introduction
A Region identifies a specific population of cells.
Up to 256 regions (total) can be set on any combination of Parameters, 32 of these are usable as
Gates.
Regions are drawn on a Single Parameter Histogram by setting the position of an upper and lower
boundary (Region markers).
Regions are drawn on a 2-Dimensional Dot Plot by enclosing a subpopulation of events with
rectangles, polygons (nonrectangular Regions) or using quadrant markers (four rectangular
Regions, set with only one point).
To speed up the analysis of data, when a Single Parameter Histogram Region or a Rectangular or
Polygonal Region is created, it is also assigned to a Gate. This assignment can be accepted or edited
later. The automatic assignment of Regions to Gate can be switched on or off if desired, using
Gates >
Automatic Gate Creation .
Region Properties
on a region to make it active and then click the right mouse button on it to display the Region
Properties dialog box. .
The
Re-Calculate AutoGate
option allows a return to AutoGating after manual manipulation of an
Auto region. All the AutoGates are re-calculated, including positives analysis in a protocol. Autogate
functionality, including Positive Analysis, is suspended whenever the gate or positive analysis
region is edited during acquisition.
Polygonal Region
The Polygonal Region allows you to draw a free-form Region with up to 100 points and can include
horizontal acute angles on a dual parameter plot. To draw a Polygonal Region in a dual parameter
display see,
Polygonal Region .