Beckman Coulter Airfuge Page 1 Instruction Manual Download

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AF-IM-13

Instruction Manual

Airfuge

Air-Driven Ultracentrifuge

Summary of Contents for Airfuge

Page 1: ...AF IM 13 Instruction Manual Airfuge Air Driven Ultracentrifuge...

Page 2: ...lter les documents joints Atenci n consulte los documentos adjuntos Attenzione consultare le informazioni allegate On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione...

Page 3: ...on this instrument Electrical Safety To reduce the risk of electrical shock this instrument uses a three wire electrical cord and plug to connect this equipment to earth ground To preserve this safet...

Page 4: ...not be used in this ultracentrifuge however unless all necessary safety precautions are taken Observe all cautionary information printed on the original solution containers prior to their use Handle...

Page 5: ...AF IM 13 December 2007 Instruction Manual Airfuge Air Driven Ultracentrifuge 2007 Beckman Coulter Inc...

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Page 7: ...cycling Label x SECTION 1 DESCRIPTION Functional Description 1 1 Safety Features 1 2 Controls and Indicators 1 2 Pressure Regulator Knob 1 2 Pressure Gauge 1 2 Run Light 1 2 Time Delay Knob 1 2 Brake...

Page 8: ...Starting the Run 3 2 Ending the Run 3 4 SECTION 4 TROUBLESHOOTING SECTION 5 MAINTENANCE Instrument Care 5 1 General Maintenance 5 2 Cleaning 5 2 Instrument Interior and Exterior 5 3 Stator Assembly 5...

Page 9: ...2 Aligning the Brake Pin in the Stator 2 5 Figure 2 3 Cold Room Set up 2 7 Figure 3 1 Worn and New Stator Pads 3 3 Figure 5 1 Prying Up the Stator 5 5 Figure 5 2 Lifting Out the Stator 5 5 Figure 5 3...

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Page 11: ...maintenance We recommend that you read this entire manual especially the SAFETY NOTICE and all safety related infor mation before operating the ultracentrifuge or performing instrument maintenance Se...

Page 12: ...r CONVENTIONS Certain symbols are used in this manual to call out safety related and other important information These international symbols may also be displayed on the ultracentrifuge and are reprod...

Page 13: ...This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of FCC Rules These limits are designed to provide reasonable protection against harm...

Page 14: ...onic Equipment WEEE Directive of the European Union The presence of this marking on the product indicates 1 the device was put on the European market after August 13 2005 and 2 the device is not to be...

Page 15: ...Rotor speed can be deter mined by conversion of the applied air pressure as displayed on the PRESSURE gauge to rpm 1 An available Digital Speed Readout Accessory 347592 which attaches to the top of th...

Page 16: ...and turning to the right clockwise closes the door Turning the knob further brings up the air pressure and accelerates the rotor to speed PRESSURE GAUGE Operating air pressure is displayed on this ga...

Page 17: ...w located on the back panel see Figure 1 4 may be necessary LEVITATION AIR SCREW When the timer reaches zero at the end of a run the driving air is turned off and levitation air supports the rotor dur...

Page 18: ...igure 1 3 Cross section View of the Airfuge Figure 1 4 Rear View Instrument Door Protective Window Turbine Flutes Levitation Air Passage Brake Pin Electromagnet Stator Stator Pad Driving Air Passage B...

Page 19: ...tor speed and the air jets that levitate the rotor during deceleration Levitation air operates automatically during deceleration STATOR PAD The black stator pad see Figure 1 5 rests on the channel of...

Page 20: ...tes to a stop DOOR LATCH The door latch mechanism secures the door during centrifugation This prevents premature door opening which would result in damage to the rotor and stator pad FILTER A filter i...

Page 21: ...g Air pressure Air pressure at ultracentrifuge for routine operation 30 psig 207 kPa Air pressure at filter for routine operation 42 psig 290 kPa Air pressure drop across filter 12 psig 83 kPa Maximum...

Page 22: ...s at a tube angle of 30 degrees from the axis of rotation and is ideal for the efficient sedimentation of small sample volumes in a very short time Maximum operating speed is 92 000 rpm at 30 psig 207...

Page 23: ...ance boundary while the ultracentrifuge is operating except to change operating controls if required Select a location away from heat producing laboratory equipment with sufficient ventilation to allo...

Page 24: ...ower cord Never use a three to two wire plug adapter Never use a two wire extension cord or a two wire non grounding type of multiple outlet receptacle strip If there is any question about voltage hav...

Page 25: ...he quick disconnect hose into the sleeve until they snap together see Figure 2 1 NOTE Do not install the filter upside down The filter bottom has a valve that distinguishes it from the top Escaping ai...

Page 26: ...air source The air flow arrow must point toward the ultracentrifuge 3 Connect the quick disconnect hose to the fitting at the rear of the instrument Place a beaker or other container under the filter...

Page 27: ...approximately 6 mm above the stator gently turn the pin so that it drops down to straddle the spring wire 3 Install the black ring shaped stator pad in the channel of the stator Figure 2 2 Aligning t...

Page 28: ...rotor takes too long to stop the brake pin tension or levitation air needs adjusting See Section 5 for adjustment procedures COLD ROOM INSTALLATION If rotor temperatures below room temperature are req...

Page 29: ...t will be a few degrees higher than the cold room temperature the rotor will typically be operating at approximately 8 to 10 C Figure 2 3 Cold Room Set up Compressor or Laboratory Air Supply Compresso...

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Page 31: ...in systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good labora tory procedures and methods to prevent spread of disease Because spills may...

Page 32: ...Tubes should be symmetrically loaded in a fixed angle rotor rotor liners should be full and evenly seated in their rotor base NOTE If you are using an Airfuge with the digital speed readout accessory...

Page 33: ...the dial past the 30 minute point then back to the required time ACR 90 rotor set the run time for 10 minutes to prevent significant evaporative loss 7 Secure the instrument door by turning the press...

Page 34: ...run light goes out and the brake pin engages the rotor The rotor will stop about 1 to 3 minutes later depending on the rotor NOTE If the coasting time is too short 45 seconds or less check the time de...

Page 35: ...malfunctions are described in Table 4 1 along with possible causes listed in the probable order of occurrence and corrective actions Perform the recommended corrective action in sequence as listed If...

Page 36: ...runs erratically or makes unusual noise Stator pad damaged Replace the stator pad Dirty air jets Clean the stator assembly Missing or damaged rotor cap Check the rotor cap Rotor improperly loaded Che...

Page 37: ...uit breaker tripped Reset the circuit breaker Time delay faulty Call Beckman Coulter Field Service Rotor crashes Stator pad worn Order Repair Kit 1 Missing rotor lid or cap Refer to the applicable rot...

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Page 39: ...NSTRUMENT CARE NOTE It is your responsibility to decontaminate the instrument as well as any rotors and or accesso ries before requesting service by Beckman Coulter Field Service representatives WARNI...

Page 40: ...ost its cushioning effect and will eventually cause rotor failure Replace a worn pad NOTE Proper deceleration of the rotor that is allowing the rotor to come to a complete stop before opening the door...

Page 41: ...he rear of the instrument by pulling back the quick disconnect hose sleeve The hose may be discon nected while it is under pressure but be careful not to damage surrounding objects 2 Remove the stator...

Page 42: ...tight 10 Place the stator pad on the channel of the stator 11 Reconnect the power cord 12 To reconnect the air supply pull back the spring loaded sleeve of the quick disconnect hose and push the slee...

Page 43: ...eep the stator level to prevent binding Figure 5 1 Prying Up the Stator Figure 5 3 Stator O Ring Positions Figure 5 4 Reassembling the Stator and Pin Brake Pin Stator Screw Brake Spring Wire Brake Spr...

Page 44: ...verage point for the stator tools 4 Gently push the wire to the center using a screwdriver blade If the wire is too far off center to be engaged by a tool or if it will not stay centered reposition it...

Page 45: ...d clean it with alcohol then reinstall it 2 Turn the BRAKE TENSION screw see Figure 5 6 clockwise decrease as viewed from the instrument back until the brake pin drops to the stator surface 3 Turn the...

Page 46: ...ill engage the braking system and hold the brake pin down for 2 minutes while you complete this adjustment 3 Place a rotor on the stator pad Be sure rotor caps or lids are properly installed The rotor...

Page 47: ...f the 2 minute period the run light will go out and a clicking sound can be heard when the brake pin is released TIME DELAY PERIOD ADJUSTMENT The delay period between the time the timer reaches zero a...

Page 48: ...eld Service to ensure that the decontamination method does not damage any part of the instrument or accessories STERILIZATION AND DISINFECTION Instrument surfaces are finished with vinyl paint the win...

Page 49: ...742 2345 in the United States worldwide offices are listed on the back cover of this manual for information about ordering parts and supplies A partial list of supplies is given below for your conveni...

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Page 51: ...ges prepaid and will be returned to Buyer with the trans portation charges collect unless the product is found to be defective in which case Beckman Coulter will pay all transpor tation charges CONDIT...

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