BD FACSymphony A3, User Manual

Page: 50 / 128

User’s Guide 50
For the cleaning solution, use BD FACSClean solution. See
Maintenance overview (page 48) for other recommended
cleaning solutions.
3. Move the tube support arm under the tube (vacuum off) and
allow the cleaning solution to run for 5 minutes with the
sample flow rate set to HIGH.
4. Repeat steps 2 and 3 with BD FACSRinse solution.
5. Repeat steps 2 and 3 with DI water.
6. Press the STANDBY button on the fluidics control panel.
7. Place a tube containing no more than 1 mL of DI water on the
SIP.
A tube with 1 mL of DI water should remain on the SIP to
prevent salt deposits from forming in the injection tube. This
tube also catches back drips from the flow cell.
Caution! Do not leave more than 1 mL of water on
the SIP. When the instrument is turned off or left in
standby mode, a small amount of fluid will drip back
into the sample tube. If there is too much fluid in the
tube, it could overflow and affect the cytometer
performance.