3
BD FACSort System
337452 Rev. A
Fluidics Control Panel
This panel consists of the fluidics control dial and the sample flow rate buttons.
Fluidics Control Dial
This rotary dial allows you to select from five fluidic modes.
DRAIN
—forces liquids out of the flow cell and fluid lines leading to the waste.
FILL
—fills the flow cell and fluid lines with sheath fluid at a controlled rate, which
prevents bubble formation.
RUN
—pressurizes the sample tube to transport the cell sample suspension through the
sample injection tube and into the flow cell. When a tube is not on the Sample Injection
Port (SIP), the cytometer goes into auto Standby. Sheath flow is restricted and laser
power is lowered.
STANDBY
—stops sheath flow and lowers laser power to conserve sheath fluid and
prolong laser life
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Hard STANDBY
—When the fluid control dial is manually turned to STANDBY,
all fluid valves are closed except for one valve that vents the sample test tube air
pressure to the atmosphere. Additionally, the laser input current is reduced to a
standby level.
•
Auto STANDBY
—If the instrument is in RUN and the sample test tube is
removed, the instrument goes into autostandby. Twelve seconds later, the system
shuts off the sheath flow by means of a pinch valve between the flow cell and the
waste reservoir. Additionally, the laser input current is reduced to a standby level.
In this mode, back pressure is maintained to flush the sample injection tube. The
system returns to RUN mode when you reinstall the sample tube. The pinch valve
is opened and the laser returns to full power.
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