Flushing the System
Fill the sheath tank with 2 L of undiluted BD FACSClean™ Solution or
a freshly prepared 10% bleach solution.
Connect the sheath tank. Open the roller clamp on the sheath line
for about 10 seconds and drain the fluid into a beaker. Close the
roller clamp.
Remove the DI water tube from the SIP and with the tube support
arm to the side, press the PRIME button on the fluidics control panel
and when the STANDBY lights, press PRIME again.
Install a tube with 3 mL of undiluted BD FACSClean™ Solution or a
freshly prepared 10% bleach solution on the SIP with tube support
arm underneath the tube and press RUN and HIGH on the cytometer
fluidics control panel. Run for 30 minutes.
Press the STANDBY button, disconnect the air line (green) from the
sheath tank and depressurize the sheath tank by lifting the vent
valve. Dispose of any remaining solution and rinse the sheath tank
with DI water.
Repeat steps 1 through 5 using DI water in the sheath tank and on
the SIP to flush out the BD FACSClean™ Solution or 10% bleach.
NOTE
Make sure to rinse the sheath tank with DI water and empty
the waste tank before adding the next solution.
CAUTION
Never mix BD
®
Detergent Solution or bleach because
they can create chlorine gas.
Repeat steps 1 through 5 now using 1.5% BD
®
Detergent Solution,
(dilute in DI water from concentrate) in the sheath tank and on the
SIP.
Repeat steps 1 through 5 now using DI water in the sheath tank and
on the SIP to flush out the detergent solution.
Reconnect the sheath filter and refill the sheath tank with sheath
fluid.
Check for bubbles and purge the sheath line or sheath filter, if
needed.
Class I Laser product
This material is for training purposes.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
BD-72621 (v1.0)
BD Life Sciences, San Jose, CA, 95131, USA
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BD, the BD Logo, BD FACSCelesta, BD FACSClean, BD FACSFlow, BD FACSymphony and BD LSRFortessa are trademarks of Becton,
Dickinson and Company or its affiliates. ©2022 BD. All rights reserved.
Air line
Fluid line