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P-OQ0612-5291-E03 

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Operational 

Q u alification  (O Q

 

 

 

Automatic Polarimeter 

AP-300 

 

Operational Qualification of Automatic Polarimeter AP-300 

 

 

Preface 

  

 

The Operational Qualification performance test of the Automatic Polarimeter AP-300 is described 

herewith. 

The instructions below assume that the performance test of the AP-300 is conducted by a regular 

user of the unit who is familiar with the operation. For daily check and maintenance, please refer 

to the Standard Operational Procedure document (SOP). 

 

 

1. Test Environment and Preparation 

 

Conduct the performance test in an environment which meets the conditions outlined below. 

Keep the measurement sample and the AP-300 at an ambient temperature of 18.0°C to 22.0°C 

for at least 30 minutes to let them acclimate to the ambient temperature. 

If the following environmental conditions are not  met, an appropriate measurement result 

may not be obtained. 

 

Environment for performance test 

 

 

             

 

 

 

 

 

2. Establishing a Discretionary Measurement Standard   

 

A performance test requires a discretionary measurement standard. 

Establish a set measurement standard  for your samples, taking the accuracy of the AP-300 

into account. Please refer to the product literature  and/ or  the  instruction manual for  the 

specifications,  features,  and measurement accuracy of the AP-300. These specifications  are 

guaranteed at the time the AP-300 is shipped as a brand-new product. It is difficult to obtain 

an accuracy level greater than the product specifications  under different environmental 

conditions. It is recommended setting the discretionary measurement standard lower than the 

stated specifications. 

 

Example: Judgment standard measurement 

             Accuracy: Angle of rotation ±0.03° 

 

 

3. Confirmation of Measurement Accuracy (Accuracy and Repeatability) 

 

Check the accuracy (accuracy and repeatability) of the measurement values by measuring a 

sample of a known concentration. 

The AP-300 is an optical instrument designed to measure the angle of rotation of a sample 

solution by utilizing two polarizing plates.   

Ambient temperature: 18.0°C to 22.0°C     Relative humidity: less than 75% 

Power supply: AC 100 to 240V, 50/ 60Hz 

 

The Automatic Polarimeter AP-300 should be used indoors and placed on a flat, 

level surface, such as a work desk or laboratory table. 

Summary of Contents for AP-300

Page 1: ...tionary measurement standard Establish a set measurement standard for your samples taking the accuracy of the AP 300 into account Please refer to the product literature and or the instruction manual for the specifications features and measurement accuracy of the AP 300 These specifications are guaranteed at the time the AP 300 is shipped as a brand new product It is difficult to obtain an accuracy...

Page 2: ...ailable prepare a standard sucrose solution of 100 Z Refer to below in a 200mm observation tube 3 Recording chart for measurement data How to make standard sucrose solution of 100 Z Put 26 000g of pure sucrose in a graduated cylinder and add distilled water to the pure sucrose until the total volume is 100ml 5 Preparation of Instrument 1 Turn on the power of the AP 300 The measurement screen appea...

Page 3: ...Check if they are consistent 2 The AP 300 stores the measurement value of the last 30 measurements in the memory If the number of measurements exceeds 30 the newest measurement values will replace the oldest measurement values 3 The measurement values stored in the memory can be recalled on the screen to compare with those in the measurement data recording chart 4 The measurement values stored in ...

Page 4: ...z control plate 34 Part number RE 72044 Quartz control plate 17 Part number RE 72043 Quartz control plate 8 Use a Quartz control plate whose angle is close to the angle of rotation usually measured 2 If a quartz control plate is not available fill a 200mm observation tube with a prepare a standard sucrose solution of 100 Z 3 Function Examination After a performance test in MODE 1 the measurement s...

Page 5: ...key 5 The display returns to the SET UP MENU screen Press the QUIT key The display returns to the measurement screen 6 Set a standard Quartz control plate or a standard sucrose solution of 100 Z in the sample chamber 7 Close the sample chamber cover Press the START key 8 OUT OF RANGE will be displayed after measurement is completed TEMP ERROR 1 Press the SCALE key in the measurement screen to disp...

Page 6: ...ress the MENU key in the measurement screen to display SET UP MENU 2 2 Select 7 SYSTEM LEVEL and press the ENTER key The SYSTEM LEVEL screen appears Make sure the SYSTEM LEVEL is 0 Press the QUIT key The screen returns to SET UP MENU 2 2 Press the QUIT key again to return to the measurement screen ...

Page 7: ...st Average value Difference Judgment standard Accuracy Angle of rotation 0 03 2 Function Examination Functions Contents Switch function Power supply switch Key functions ZERO QUIT MENU SCALE START ENTER Arrow keys NUMERIC keypad Display functions Opening message Date and time Error messages ZERO SET END OUT OF RANGE TEMP ERROR Output functions Printer output RS 232C output check each box 3 Evaluat...

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