Arthrex Angel, Operator'S Manual

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Programmability Option
Angel ® cPRP System Operator’s Manual
4-1
Chapter 4: Programmability Option
PRECAUTION
The operator of the Angel System is responsible for monitoring the
system performance when using custom protocols.
Creating Custom Protocols
The Angel System is provided with a single Standard Protocol to separate the whole
blood or mixture of blood and bone marrow into red cells, PPP and PRP. The
parameters of the Standard Protocol have been extensively tested and optimized to
produce reproducible results. By selecting the “ Protocols ” tab of the Menu Screen
(see Figure 4-1), custom protocols may also be created that have different spin speeds
and times, or that yield a PRP product containing a different proportion of red
blood cells.
During blood separation using the
Angel System
Standard Protocol, the anticoagulated
whole blood or the mixture of blood and bone marrow is pumped into the Angel
System’s spinning Variable Volume Separation Chamber. The red blood cells, the
heaviest elements of the blood, are separated from the lighter elements, the plasma
and platelets, through centrifugation. The Angel System Standard Protocol uses a
two-phase spin cycle consisting of a hard spin and a soft spin. The Standard cycle
varies the RPM and Time for this two-phase spin cycle based on the volume of whole
blood/bone marrow processed. On the custom Protocol screen, the Spin 1 and Spin
2 values for RPM and Time can be changed.
The first product that is collected after the two-phase cycle is complete is the PPP. The
PPP is pumped from the spinning Variable Volume Separation Chamber into the PPP
Compartment. When the sensor of the
Angel System
senses the presence of the PRP
layer, the Rotating Valve changes position and directs the PRP product to the PRP
syringe along with an additional volume of RBC. The additional volume of RBC
maximizes the recovery of platelets. At the end of the PRP collection phase, the
Rotating Valve changes its position to the RBC Compartment, and empties the
remaining volume.
If the PRP volume collected (in the 20 mL Luer Lock Syringe) is diluted to a volume
equal to (approximately) 10% of the processed blood/bone marrow volume, the
Angel System Standard Protocol will yield a product with approximately a 7%
hematocrit. The Protocol Screen allows the creation of custom protocols for
increased or decreased volumes of RBC volume in the final PRP product.
Since young platelets extend into the RBC layer, a change in volume of RBC in the final
PRP product will also change the final concentration of platelets in the PRP product.
Changes in the RPMs and Times of Spin 1 and Spin 2 may also change the
concentration of platelets in the final PRP product. The change in concentration
and collection of platelets when using a custom protocol is the responsibility of the
Angel System operator. The operator should be knowledgeable in the methodology
of blood component separation, prior to operating a custom protocol.