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Applied Photophysics RX2000, User Manual

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Applied Photophysics RX2000 User Manual Download Page 10

 
 

Document  4400Q902  version 1.02 

 

Page 10 of 18 

 

RX2000 User Manual 

2 HARDWARE 

This Chapter describes the RX2000 hardware, covering the basic layout and main components of the accessory. 

2.1 

Basic layout 

 

 

 

Figure 2.1: schematic of the RX2000 

 
 
A schematic of the RX2000 is shown in Figure 

2.1

. The unit is designed to be compact and easy to use,  allowing 

stopped-flow measurements to be obtained with UV-visible spectrometers, fluorimeters and CD polarimeters with 
the  minimum  amount  of  setting-up.  It  comprises  a  base  in  which  the  electronics,  battery  and  LCD  temperature 
display panel are located. Attached to the top of the base is a reagent handling system with thermostat bath and 
this  is  connected  to  a  specialised  cuvette  via  an  umbilical.  The  sealed  thermostat  bath  incorporates  a  window 

Summary of Contents for RX2000

Page 1: ...User Manual RX2000 Rapid Mixing Stopped Flow Unit April 2013 Document 4400Q902 version 1 02 ...

Page 2: ...Document 4400Q902 version 1 02 Page 2 of 18 RX2000 User Manual This page is intentionally left blank ...

Page 3: ...02 version 1 02 Page 3 of 18 RX2000 User Manual This document contains important safety information Read this document before attempting to install or use the RX2000 Failure to do so could result in injury to the user ...

Page 4: ...ed Photophysics be held responsible for incidental or consequential damages with or arising from the use of this document COPYRIGHT 2013 APPLIED PHOTOPHYSICS LTD ALL RIGHTS RESERVED THIS DOCUMENT OR PARTS THEREOF SHALL NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER RX2000 is a trademark of Applied Photophysics Ltd Norton is a trademark of Symantec Corporation or its ...

Page 5: ...y OTHER INFORMATORY INDICATORS USED IN THIS DOCUMENT The sign to the left is used to indicate a situation which if not avoided could result in damage to the instrument HAZARD INDICATORS USED ON THE RX2000 Note that this hazard indicator may be either coloured as below or as black and white The sign to the left is a general hazard indicator indicating the presence of a hazard that is either describ...

Page 6: ...FORMANCE OF INSTALLATION OPERATION OR MAINTENANCE PROCEDURES OTHER THAN THOSE DESCRIBED IN THIS USER MANUAL MAY RESULT IN A HAZARDOUS SITUATION AND WILL VOID THE MANUFACTURERS WARRANTY The RX2000 pneumatic accessory uses compressed gas to drive the drive syringes and could trap hands or clothing causing injury to the user Keep hands and clothing clear when performing a drive using the accessory Co...

Page 7: ...condensing Bench space The RX2000 requires a bench space of Length x depth x height 0 7 x 0 3 x 0 2 metres alongside the spectrometer with which it is used A sturdy stable vibration free work surface is recommended Fluid circulator unit The RX2000 can be used with or without temperature control Temperature control is provided by a standard laboratory temperature controlled fluid circulator Servici...

Page 8: ...mperature control 14 3 1 3 Trigger signal 14 3 2 Operation using the manual drive 14 3 3 Operation using the pneumatic drive accessory 15 3 4 Operation using the anaerobic accessory 16 3 4 1 Mounting the anaerobic accessory 16 3 4 2 Anaerobic operation 16 3 5 Operation using ratio mixing 17 3 6 Changing the drive syringe 17 3 7 Selected test reactions 17 3 7 1 Formation of Iron III Thiocyante 17 3...

Page 9: ...tect the instrument from aggressive chemicals The entire flow circuit and the drive syringes themselves are located within a thermostatable bath for accurate sample temperature control The stopped flow technique for the rapid mixing of chemical reagents has gained widespread acceptance as being a most useful and versatile method for obtaining reaction rate information After the two reagents are mi...

Page 10: ...it is designed to be compact and easy to use allowing stopped flow measurements to be obtained with UV visible spectrometers fluorimeters and CD polarimeters with the minimum amount of setting up It comprises a base in which the electronics battery and LCD temperature display panel are located Attached to the top of the base is a reagent handling system with thermostat bath and this is connected t...

Page 11: ...ses three specially designed flow distribution valves two of which are used to load the drive syringes and the third to control the removal of waste solution from the flow line The two drive syringes have screw thread location whereas the reservoir stopping and waste syringes use Luer location The displacement of the stopping syringe piston is controlled by an adjustable stop bar on which is mount...

Page 12: ...tion open to the stopping syringe and the normal empty position open to the stopping syringe and waste reservoir Flow line closed Flow line open to waste reservoir used when flushing Figure 2 3 flow line closed left and open to waste reservoir right Flow line in its normal drive position Flow line in its normal empty position open to stopping syringe open to stopping syringe and waste reservoir Fi...

Page 13: ...yed on a LCD 2 6 Accessories The RX2000 Stopped Flow Mixing Accessory can be upgraded with a pneumatic drive attachment which may be used as required to provide powerful and reproducible stopped flow drives An optional anaerobic accessory is available to facilitate the rigorous exclusion of oxygen from the flow circuit if desired 2 7 RX2000 specifications DuoCell Purpose designed micro cell fitted...

Page 14: ...n the RX2000 and the GP IO connector on the spectrometer It will be necessary to activate external triggering of the spectrometer in the control software before using the RX2000 3 2 Operation using the manual drive The following step by step procedure describes the use of the syringes and the various valves during reagent loading flowline flushing and stopped flow drives 1 Attach the waste reservo...

Page 15: ... 3 3 Operation using the pneumatic drive accessory The RX2000 pneumatic accessory uses compressed gas to drive the drive syringes and could trap hands or clothing causing injury to the user Keep hands and clothing clear when performing a drive using the accessory The pneumatic drive assembly comprises a baseplate mounted control box with a pressure regulator push button valve and linear actuator I...

Page 16: ...s and can diffuse through FEP quite readily Without the following precautions carefully prepared deoxygenated reagents will take up oxygen from the FEP material used in the RX2000 Stopped Flow Mixing Accessory For anaerobic operation it is necessary to use a closed circuit thermostat system The Mixing Accessory thermostat bath should be connected via a suitable pump to a coiled heat exchanger imme...

Page 17: ... syringe fairly strong hand tightening should be sufficient to seal 4 If you are replacing the standard 2 5 ml syringe with a smaller syringe the smaller syringe diameter will not seal against the grommet required to prevent leakage when thermostatting The simplest solution here is to wrap insulating or electrical tape around the small syringe at the point where it seals with grommet This collar w...

Page 18: ...79 Here we suggest slightly different concentrations in order to slow the reaction down React 10 µM N acetyltryptophanamide NATA under pseudo first order conditions with 100 to 400 µM N bromosuccinimide NBS to quench fluorescence Prepare the solutions in 100 mM phosphate buffer at pH7 The fluorescence is excited at 281 nm and the emission maximum is around 360 nm A single fluorescence decay will b...

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