Applied Biosystems GeneAmp PCR System 9700, User Manual

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Converting Hold Times 6-5
Table of Conversions
The following table lists the times for converting down ramp hold times greater than
one minute from the DNA Thermal Cycler or the DNA Thermal Cycler 480 to the
GeneAmp PCR System 9700.
Down Ramp
Example
In the example below, the temperature was decreased by 22 °C. This value was
rounded to 20 °C. According to Table 6-2, with a starting temperature of 94 °C, we
should subtract 53 seconds from the hold time on the DNA Thermal Cycler or the DNA
Thermal Cycler 480, resulting in a new hold time of 27 seconds.
Note If the methods developed on the GeneAmp PCR System 9700 will be used on the DNA
Thermal Cycler or the DNA Thermal Cycler 480, use Table 6-2 to convert the hold times. Add
the indicated times instead of subtracting them.
Table 6-2 Converting down ramp hold times
A Temp (
°
C)
Starting temperature of ramp (
°
C)
35-55
°
56-75
°
76-96
°
10
°
39 sec. 39 sec. 40 sec.
15
°
45 sec. 47 sec. 48 sec.
20
°
49 sec. 52 sec. 53 sec.
30
°
— 56 sec. 59 sec.
40
°
— 57 sec. 62 sec.
50
°
— — 62 sec.
60
°
— — 60 sec.
80 sec.
Hold
Time
94
72
Temp.
(
˚
C)
Starting Temp=94˚ Starting Temp=94
˚
∆
temp=22 ˚
(round to 20 ˚ )
DNA Thermal Cycler
27 sec.
Hold
Time
94
72
Temp.
(
˚
C)
GeneAmp PCR System 9700