78
Thermal Cycler Temperature Verification System For GeneAmp
®
PCR Systems 2720, 9600, 9700, 9800,
and Veriti
®
System User Guide
Chapter 7
Calibration verification test
Calibration verification test
Use the calibration verification test to verify the temperature calibration of your
GeneAmp
®
PCR System 9700
, 0.5-mL
Sample Block Module
.
The calibration
verification test consists of the following procedures, which you must perform in
sequence:
Setting up the 0.5-mL probe assembly
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Required
equipment
This test requires the 0.5-mL Sample Block Module Temperature Verification Kit
(PN 4309924), which includes:
• Cotton swabs
• Light mineral oil
• GeneAmp
®
0.5-mL temperature verification frame
• 0.5-mL probe assembly
• Digital thermometer Model 4500 with a 9V battery installed
Setting up the
0.5-mL probe
assembly
1. If the heated cover is in the forward position, lift the lever, then slide the heated
cover back.
2. Place the GeneAmp
®
0.5-mL temperature verification frame on the sample
block.
3. Use a cotton swab to coat well A6 with mineral oil.
4. Place the 0.5-mL probe assembly into well A6.
5. Thread the probe wire through the channel in the 0.5-mL temperature
verification frame to prevent damage to the probe and lead wires.
6. Make sure the probe is connected to the digital thermometer.
7. Slide the heated cover forward and pull the lever down.
IMPORTANT!
Seat the probe properly, and close the heated cover carefully. If
the probe wire is crushed when you close the heated cover, the probe may be
damaged.
8. Power on the digital thermometer.
Refer to the instructions in your Temperature Verification Kit for details on
operating the digital thermometer, Model 4500.
.
