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9

 

PM200 Series

 

Operation

 

 

 

 

Setting Up

 

1.

 

Once you have placed your sample on the table or base below the objective, center the sample as best as possible. 

A slide is neither needed nor required for a stereo microscope, although they can be used if desired. If using a slide, 
use the stage clips to hold it in place.

 

 

2.

 

With both eyes open, look into the eyepieces. Adjust the interpupillary distance by holding the eyepiece tubes 

and rotating the eyepiece tubes either towards or away from each other until only one circle of light is seen by both 
eyes.

 

 

3.

 

Adjust the magnification knob to the lowest setting (0.7) and move the microscope’s focusing knobs (on the 

focusing rack) to adjust the height of the unit until at approximately 4 inches (101mm) away from the sample. If 
not clear after adjusting, please be sure that you have not installed any additional Barlow lenses onto the objective.

 

 

4.

 

Center the object in the field of view once you have achieved focus.

 

 

Focusing

 

1.

 

A stereo microscope’s focus differs from a compound microscope. Magnification and focal distance (working 

distance) are inversely related to each other. The more magnified one is, the lower the distance between the objec- 
tive and the sample to achieve focus.

 

 

2.

 

When first using the microscope, remove all Barlow lenses and set the magnification knob (on the microscope 

head) to the minimum setting (0.8x).

 

 

3.

 

Begin focusing the microscope by moving the knob until the head is about 4 inches (101mm) away from the 

sample. This should bring the microscope in focus at 8x.

 

 

4.

 

If you’d like to use greater magnification than the minimum setting, you will need to move the microscope head 

closer to the sample as well as adjust the magnification knob to a higher setting. The more magnified, the closer 
you need to be.

 

 

5.

 

This unit  is a  common main  objective (CMO) microscope, so  the optical  quality through  the trinocular  port 

and eyepieces will be better, however the image will appear more flat than your typical Greenough design stereo 
microscope. The trinocular port when using a camera will appear to have more focus than the Greenough variants 
due to the single objective design (rather than the dual offset design of a Greenough).

 

Summary of Contents for PM200 Series

Page 1: ...PM200 Series 1 User s Manual ...

Page 2: ...rted 8 Assembly 8 Operation 9 Setting Up 9 Focusing 9 Using the Trinocular Port 10 Attaching a Camera 10 Adjusting Focusing Tension 11 Maintenance Precautions 11 Specifications 12 PM200 Series Specifications 12 Optional Accessories 13 Technical Terms Concepts 14 Troubleshooting 15 Common Issues Optical 15 Common Issues Mechanical 16 ...

Page 3: ...cular tubes with a third port on top for a microscope camera Please take a few minutes to familiarize yourself with the features and functions of your new mi croscope If you d like more information on microscopes parts or accessories please visit our website at www iScopeCorp com We highly recommend you study this manual thoroughly before operating the microscope and that you keep it on hand for f...

Page 4: ...ht or in high heat Keep it indoors in a dry and clean place with temperatures between 32 100 degrees F 0 40 degrees C and in maximum relative humidity of 85 3 Avoid touching the lenses on the objectives and the eyepieces so that oil and dirt from your fingerprints do not obstruct your view 4 Before turning the power on make sure that the power supply voltage is consistent with the voltage of your ...

Page 5: ...Series Parts PM200T Trinocular Port PM220T Only Eyepieces Ocular Tubes Interpupillary Adjusters Pillar Stand Magnification Knob Objective FocusingRack Focusing Knob Top Light Source StageClips Base Illuminator ...

Page 6: ...nge Dimmer Controls the amount of light that escapes from the illumination source Diopter Allows the focus to be perfected for both eyes independent of each other Fixed Power Microscope head whose objectives are pre set atcertain levels and changed by rotating the objective FocusingKnob Used to move the microscope head vertically to bring the sample into focus GooseneckIlluminator Illuminator inde...

Page 7: ...Ocular Tube Tube designed to hold the eyepieces or camera in place Ring Light An illuminator that mounts directly onto the objective to emit even light on the sample below Simul FocalMicroscopeHead Head containing a focus adjustment for a trinocular port camera to simultaneously to match the eyepieces Table Stand A stand which sits on the table and holds the head directly above the sample without ...

Page 8: ...base that comes with your unit the focusing rack and the head lock screw 4 After mounting the focusing rack to the base if not already attached in shipping slide the objective of the microscope into the hole and secure with included head lock screw 5 For the PM200T models screw the photo port onto the top of the microscope head trinocular C mount port 6 Remove the eyetube caps and drop the desired...

Page 9: ...ocus differs from a compound microscope Magnification and focal distance working distance are inversely related to each other The more magnified one is the lower the distance between the objec tive and the sample to achieve focus 2 When first using the microscope remove all Barlow lenses and set the magnification knob on the microscope head to the minimum setting 0 8x 3 Begin focusing the microsco...

Page 10: ... focal microscope head one with a focusing adjustment on the third port then you can use the middle portion to focus the camera This allows the camera to be in focus even if the magnifications between the eyepieces and the camera are different The PM200 models do not come standard with a simul focal feature Attaching a Camera 1 If your microscope has a C mount you can simply screw the camera onto ...

Page 11: ... on the optical surface should be blown off using a hand blower or gently wiped off with a soft lens paper tissue nonabrasive lint free cloth Carefully wipe off oil or fingerprints on the lens surfaces using tissue moistened with a small amount of lens cleaner we recommend Sparkle brand optical cleaner Do not use Sparkle to clean other elements of the microscope Use a neutral detergent on any plas...

Page 12: ... WF10X 18mm x x WF10X 18mm w Pointer WF10X 18mmw Reticle WF16X 13mm WF20X 10mm WF25X 10mm 45 Degree Viewing Head Binocular Interpupillary Adjustment x Trinocular Interpupillary Adjustment x TrinocularPort C Mount Photoport x 23mm Photo Port x Stage 130mm x 140mm Mechanical Stage x x Movement Range 50mm x 76mm x x ...

Page 13: ...halfing the magnification and doubling work ing distance 2 0X SM20 For doubling the magnification and halfing the working distance Camera CMOSDigital MU035 350k MU130 1 3mp MU300 3mp MU500 5mp MU800 8mp MU900 9mp MU1000 10mp To capture images video or view live display on a computer PC Mac OS X Calibration Micrometer MR400 To calibrate the camera software for on screen measurements CCD Digital VGA...

Page 14: ...e objective lens and the specimen The α is 1 2 of the angle between the aperture on the objective and path of light The larger the N A the higher the resolution of the objective and better quality of the image is ObjecttoPrimary ImageDistance The distance between the object plane and the primary image plane The conjugate distance is fixed Mechanical Tube Length The distance between the objective s...

Page 15: ...ge appears in focus Note Higher magnification will require the head to move closer while lower will require more distance between the sample and the lens One side of the field of view is dark or the image moves while focusing The specimen is not fixed Secure the slide to the stage with clips or another manner so that the sample does not move The field of view is not bright enough The light source ...

Page 16: ...sing rack and twisting them in opposite directions Stage declinesbyitself The tension knob is too lose Loosen it by adjusting the tension by grabbing both knobs on the focusing rack and twisting them in opposite directions Thefocusing knobwon t raise lowerthehead The focusing rack has reached the max travel distance in either direction Move the focusing rack higher or lower on the pillar if using ...

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