Amos AEM 460 Operation Manual Download Page 26

 

 

AEM460 Semi-automatic

 

Microtome

 

AMOSAEM460OP20180306

 

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6

Trouble Shooting

 

Below you find a list of the problems that most frequently occur. And they are most 

caused by the operator, so please read the operation manual carefully before use it. 
 

Problem                      Possible causes                          Corrective action 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 

·

No display, no reaction   

to buttons pressed after 

the instrument is 

  switched on 

 

 

 

 

 

 

·

 

Alarm is activated after 

switching  the  instrument 

on. 

 

·

 

The section is uneven 

  from the second section 

 

 

 

 

 

·

The  section  is  not 

continuous 
 

 

 

 

 

 

 

 

 

 

 

 

 

·

Mains cable not properly connected 

  or it is break in the circuit. 

 

·

Mains fuses defective 

 

·

The input voltage is not match with 

  the voltage showed in the marks. 

 

·

The rapid-stopped switch is pressed 

 

·

The cord connect control to the 

  instrument is not or not well connected 

 

 

·

The sectioning angle of the knife is 

  too small. 

 

 

 

 

 

·

The clamping setting is not steady 

 

·

The paraffin is too hard. 

 

 

 

 

·

The  angle  between  knife  and  the 

embedded cassette is too large. 

 

·

The set sectioning thickness is too 

  Thick. 

 

·

Blunt cutting edge 

 

·

Check if mains cable is properly connected 

  or replace the main cable 

 

·

Replace the fuses 

 

·

If it is not matched, call for the professional 

personnel. 

 

·

Check rapid-stopped switch and resume it 

 

·

Reconnect the control panel to the instrument 

 

 

 

·

Alternately  thick  and  thin  sections  are 

produced.  In  extreme  case,  every  second 

section is skipped, being followed by a very 

thickness. Systematically try wider clearance 

angle setting until  optimum angle width has 

been found. 

 

·

Check if all the screws are tightened. 

 

·

Embed  the  specimen  again  in  the  low-fusing 

paraffin or drop the embedded specimen into the 

low-fusing paraffin, and then trim the embedded 

specimen to make the thin 

 

·

Decrease  the  angle  between  knife  and  the 

embedded specimen. 

 

·

Adjust the setting thickness 

 

 

·

Replace the blade or adjust the position of the 

blade.

 

 

 

 

 

Summary of Contents for AEM 460

Page 1: ...AEM 460 Version AMOSAEM460OP20180306 Semi automatic Microtome Operation Manual www amos scientific com AMOS SCIENTIFIC PTY LTD ABN37 159 778 140...

Page 2: ......

Page 3: ...and using notes Be sure to read these instructions before using the instrument Quick release cassette Clamp Knife holder Display Panel Separate control panel Handwheel Locking Mechanism Section Waste...

Page 4: ...ent is snuff long haul and safely used Our company is responsible for service after the microtome have been used our agents have ability to service the client all of world can connect with our agent i...

Page 5: ...6 3 1 Installation Site Requirement 6 3 2 Standard Delivery 6 3 3 Installation 7 3 4 Electrical Connection 8 4 Operation 9 4 1 Control Panel Function Control 9 4 2 Knife Holder inserting 12 4 3 Clamp...

Page 6: ...strument 1 2Safety Warning The following safety notes relating to transport installation calibration operation maintenance cleaning and all aspects of the instrument Every principal person must be rea...

Page 7: ...e knife guard when slicing Don t Clockwise and Counterclockwise rotate the handwheel frequently at top and bottom Slice thickness many deviations Ensure that liquids don t enter the interior of the in...

Page 8: ...2 is not activated When the lock is activated the LOCK indicator will light on display panel while the machine DON T running DON T lock the handwheel when the handwheel is rotating otherwise it will...

Page 9: ...assette Clamp Display Panel Handwheel locking Mechanism lock at fixed position Handwheel Locking mechanism lock at any position Knife Guard at knife holder Knife holder Clamping lever for knife holder...

Page 10: ...ted in the uppermost locked The electric coarse feed operates two speeds which is easy to record the feed Separate control panel is more easy to operate Automatic memorized coarse feed and reload fast...

Page 11: ...around the instrument to avoid the shelter affecting the operator operate the instrument The working temperature and humidity must be accord with the data in the manual Ensure there is enough space f...

Page 12: ...4 specimen clamp 7 front protect board 6 back protect board 3 and then the instrument 2 in turn Final to take out other accessories in the inner package to install the instrument 3 3 2 Knife holder Ta...

Page 13: ...ocal power requirements of your laboratory Connect the instrument to a grounded power socket to avoid accident It is better to use the power cable provided or use an extension cord with a ground wire...

Page 14: ...isplays Current set value of section Thickness trimming section thickness setting The second line displays section trimming counter and section thickness sum The trimming status stand by when instrume...

Page 15: ...lit up it shows the status at trimming and trimming Thickness setting SECT If its Led lit up it shows the status at sectioning and section Thickness setting RETRA If its Led lit up it shows the statu...

Page 16: ...m s Specimen feed is 28mm If exceed the value the buzzer will alarm with voice and the specimen movement stopped Button for trimming Light up when trimming mode is active Trimming and trimming thickne...

Page 17: ...and then adjust the adjustable handle of the lever 2 7 to horizontal position Rotate the rotating X axis slider 3 to slide it into the Y axis slider 2 and then insert the lever 2 8 and rotate it until...

Page 18: ...e knife before detaching the knife holder from the instrument Always put the knives back into the knife case when not in use Tighten the knife holder and the base holder by the four levers and adjust...

Page 19: ...n clamp is fit for Dimension of specimen 40 50 30mm or the standard cassette Quick release cassette clamp is fit for Standard cassette Use the screws to fix each component as Fig shows To change the s...

Page 20: ...the specimen 6 into and then free loosen lever 5 the specimen will be clamped automatically To take down the specimen just operate as above Easy to change the specimen by one hand operating the quick...

Page 21: ...the setting push the others button TRIM or SECT Trimming setting Press button while LED light up the trimming function be activate Press to select the trim thickness value displayed on Adjust the spec...

Page 22: ...se standard specimen holder and standard cassette without any exceed paraffin 2 Don t move the knife holder during the whole operation 4 6 Sectioning Sectioning setting Press button while LED light up...

Page 23: ...g and position 2 which is often contacted with the specimens especially position 2 are very easy to be polluted so need to be cleaned frequently to ensure ordinary operation Quick release cassette cla...

Page 24: ...cess the internal components of the instrument for clearance and maintenance before clearance and maintenance turn the instrument off pull out the plug and take down the knife holder and then clean al...

Page 25: ...the specified type fuse Maintenance for the knife holder Lever the fixed installations for the knife holder and other parts of the instrument which are frequently used and easily worn need to be maint...

Page 26: ...too small The clamping setting is not steady The paraffin is too hard The angle between knife and the embedded cassette is too large The set sectioning thickness is too Thick Blunt cutting edge Check...

Page 27: ...st situations Trim the embedded specimen to make the top line parallel with the bottom line and the width of the embedded specimen must be even Adjust the specimen clamping to ensure the top line and...

Page 28: ...araffin too long or the paraffin is too hot The specimen is too hard to the paraffin The blade is uneven The blade is polluted The specimen is scratched by hard particles There are calcareous or silic...

Page 29: ...olved please contact with the manufacturer 7 Instrument Diagram The surface of the section is in wave The section flies away and sticks to the microtome or other objects near the microtome The gradien...

Page 30: ...1 unit 2 Blade Holder 1 set 3 Specimen Clamp 1 set 4 Waste Tray 1 pc 5 Separate control panel 1 set With connection cable 6 M3 Allen Wrench 1 pc 7 M4 Allen Wrench 1 pc 8 Disposable blade 1 box 9 Dust...

Page 31: ...Published by AMOS SCIENTIFIC PTY LTD 7 Gabrielle Court Bayswater North VIC 3153 AUSTRALIA Tel 61 4 10190188 Fax 61 3 98081503 ABN 37 159 778 140 www amos scientific com Email info amos scientific com...

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