Running Samples
NovoCyte Quanteon Flow Cytometer Operator's Guide
85
NOTE
If you select the
Unstained
sample in the
Compensation Channel
, calculation of the
compensation matrix will be based on the unstained sample, instead of the
negative population in the single-stained sample.
NOTE
The
Compensation Channels
shown in the
New Auto Compensation
window will
be all the available channels based on the model of the NovoCyte Quanteon flow
cytometer. The NovoExpress software automatically obtains such available
channel information from the hardware. Correctly select the
Compensation
Channels
to ensure the correct automatic compensation results. You can also
change the settings by right-clicking the
Compensation Specimen
in the
Experiment Manager
(Figure 51) and select
Auto Compensation Setup ….
Figure 51.
Compensation Specimen and Related Single-Stained Samples
3
Run Samples for Automatic Fluorescence Compensation
The default setting for acquiring automatic compensation samples are:
Parameters: both Height and Area for all the available parameters
Stop condition: total 10,000 events or 20μL sample volume.
Sample flow rate: Slow
Threshold: FSC-H larger than 100,000.
NOTE
In order to ensure normal data acquisition and correct automatic fluorescence
compensation, it is recommended to prepare the compensation sample
concentration to be 1 x 10^6 ~ 2 x 10^6 /mL.
NOTE
Most samples can be properly collected with the default settings. However, users
can also change the settings accordingly.
Summary of Contents for NovoCyte Quanteon
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