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Agilent Technologies MX3000P, Setup And User Manual

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Mx3000P and Mx3005P QPCR Systems  
Setup and User’s Guide 

Mx3000P and Mx3005P QPCR Systems  Components Provided ................................................... 1

 

Setting Up the System ......................................................................................................................... 2

 

Locating the Instrument ......................................................................................................... 2

 

Environmental Conditions for Use ........................................................................................ 2

 

Unpacking the Instrument ..................................................................................................... 3

 

Installing the Fuse.................................................................................................................. 4

 

Connecting the Instrument to the PC ..................................................................................... 6

 

Configuring the PC and Installing the MxPro QPCR Software ............................................ 7

 

Performing the Instrument Qualification Test Run ............................................................. 15

 

Hardware Information and Specifications ..................................................................................... 20

 

Hardware Specifications ...................................................................................................... 20

 

Cautions and Warnings/Précautions et Avertissements ...................................................... 20

 

Instrument Status Indicator LED’s ...................................................................................... 22

 

Sample Loading ................................................................................................................................. 23

 

Thermal Block Hardware .................................................................................................... 23

 

Consumables ....................................................................................................................... 24

 

Use of the Perfect Fit Frame with Single Tubes and Strip Tubes ....................................... 24

 

Application of Caps ............................................................................................................. 26

 

Maintaining the Instrument ............................................................................................................. 27

 

Replacement Parts ............................................................................................................... 27

 

Changing the Lamp ............................................................................................................. 27

 

Replacing the Fuse .............................................................................................................. 28

 

Cleaning the Instrument ...................................................................................................... 28

 

Troubleshooting ................................................................................................................................ 29

 

Software License and Notices ........................................................................................................... 34

 

MxPro QPCR Software License Agreement ....................................................................... 34

 

Trademarks .......................................................................................................................... 36

 

Mx3000P and Mx3005P QPCR Systems Support .......................................................................... 37

 

Summary of Contents for MX3000P

Page 1: ...Mx3000P and Mx3005P QPCR Systems Setup and User s Guide...

Page 2: ...Mx3000P and Mx3005P QPCR Systems SETUP AND USER S GUIDE IN 70225 J 1 For Research Use Only Not for Use in Diagnostic Procedures...

Page 3: ...postage or shipping costs prepaid to Agilent Loss or damage during shipment shall be at the risk of the purchaser This limited warranty does not include repair or replacement necessitated by accident...

Page 4: ...Run 15 Hardware Information and Specifications 20 Hardware Specifications 20 Cautions and Warnings Pr cautions et Avertissements 20 Instrument Status Indicator LED s 22 Sample Loading 23 Thermal Block...

Page 5: ......

Page 6: ...non skirted PCR plates catalog 410088 5 Mx3000P Mx3005P optical strip caps catalog 401425 60 strips of 8 caps MxPro QPCR software installation CD 1 Installation qualification test plateb and plate set...

Page 7: ...The instrument should be installed in a location where it will not be exposed to direct sunlight or vibration during use Environmental Conditions for Use Maximum altitude 2000 m 6562 ft Ambient temper...

Page 8: ...the instrument door by pushing up on the door handle Remove and retain the foam used to immobilize the scanning head on the track 5 Move the scan arm to the left of the thermal block top 6 To access t...

Page 9: ...proper instrument operation 1 Remove the label located over the power entry module on the back of the instrument just above the power switch 2 Use a small flathead screwdriver or similar tool to open...

Page 10: ...the desired voltage is in the proper orientation If your instrument is 100 120 volts insert the fuse holder such that the 115V label is right side up If your instrument is 200 240 volts insert the fus...

Page 11: ...ded serial cable to the RS 232 serial port at the rear of the instrument If your PC has a serial port connect the free end of the cable to the serial port on the back or side of the computer If your P...

Page 12: ...n the back of the Microsoft Office installation CD sleeve and activation of the software either through an encrypted transfer over the Internet or by telephone Activation is required for exporting the...

Page 13: ...will guide you through the MxPro QPCR software setup process Click Next to continue with installation 7 A window will open with a copy of the MxPro QPCR software license agreement If you accept the te...

Page 14: ...em and then click Next 9 When asked to continue in the following dialog box click Yes Important If a NET Framework Initialization Error dialog box appears please contact Technical Services for assista...

Page 15: ...FR Part 11 Features in the installation window below A sales order number indicating purchase of these features is required to obtain an activation key If you do not require 21 CFR Part 11 compatible...

Page 16: ...activation The activation key can be obtained by contacting the technical services department by phone E mail or via the Agilent website The system generated ID sales order number instrument serial n...

Page 17: ...ormation Technology department Click Next once a database type is selected 14 Depending on the database option selected one of the following windows will appear Complete the appropriate fields either...

Page 18: ...er location using the Browse button Click Next once a folder location is selected 16 Once the MxPro QPCR software files and Microsoft SQL database software files if applicable are installed to the des...

Page 19: ...e application is opened In order to start the application enter the initial case sensitive default username Admin and case sensitive default password password Click Login 20 The first time you log in...

Page 20: ...inutes leaving the plate sealed in the foil wrapper while thawing After thawing remove the foil wrapper and then mix the contents of the wells by completing the following actions five times Invert the...

Page 21: ...ime that the MxPro QPCR software is opened using an instrument connected computer the Instrumentation Qualification Test Start dialog box will appear This dialog box may also be opened by selecting Qu...

Page 22: ...e instrument is running will abort the run If the run is accidentally aborted by opening the instrument door the following error message will appear Do you want to abort or continue the run Close the...

Page 23: ...Microsoft Excel software Validation template will open to the FAM analysis tab First verify that the Data Count cell reports the result Good ensuring that all of the data has been correctly imported...

Page 24: ...gilent com Include a contact name the name of your institution and the instrument serial number when sending the data to Technical Services See Mx3000P and Mx3005P QPCR Systems Support for additional...

Page 25: ...Mx3005P system 5 dyes per well Sample container configurations 200 l tube format configured as 96 well plates strip tubes or single tubes Reaction volume per tube 10 50 l Electrical power input 100 12...

Page 26: ...appear on screen if the door has been opened and the run has been interrupted Autres Avertissements Soyez prudents lorsque vous chargez et d chargez les chantillons du bloc thermique car ce dernier po...

Page 27: ...table below Appearance of Ready Status Indicator LED Instrument Status Continuously lit The instrument control software is operational and the unit is ready to run an experiment Blinking The unit is n...

Page 28: ...he door should not be opened Open the door located at the front of the instrument by sliding it all the way to the top To expose the thermal block lift the heated lid by pulling forward on the handle...

Page 29: ...he thermal block Important When using individual tubes or strip tubes as sample containers insert the Perfect Fit frame on the thermal block platform before loading the samples See Use of the Perfect...

Page 30: ...e will be positioned in the spaces between wells on the perimeter of the thermal block surface The Perfect Fit frame should be seated on the thermal block platform with limited freedom of movement The...

Page 31: ...well working rack Agilent Catalog 410094 Apply direct downward force to each cap and then view the rack from the side to make sure all the caps are seated evenly Alternatively caps can be applied usin...

Page 32: ...hot The lamp is located in the excitation housing on the left hand side of the instrument Before changing the lamp be sure to turn off the instrument The lamp is replaced along with the lamp housing a...

Page 33: ...To replace the fuse disconnect the instrument from the power source open the fuse housing cover and remove the fuse holder from the power entry module Replace the fuse with the appropriate fuse type...

Page 34: ...ess the software will offer the option to continue displaying data from the current run If the run completes before communication is reestablished the software should offer the option to download the...

Page 35: ...fluorophore You can test the probe by performing a nuclease digestion to ensure it is unquenching as expected incubate 100 nM of probe in 25 l of 1 buffer with 10 U of DNase or S1 nuclease at room te...

Page 36: ...material Check optical clarity of sample container cap Ensure the liquid inside that sample tube did not evaporate during the run which would indicate a vapor leak in that particular sample Vapor lea...

Page 37: ...ification When running samples with a broad dynamic range use the adaptive baseline algorithm If amplification curves still look abnormal it may be necessary to manually set the baseline ranges Baseli...

Page 38: ...ion or 400 bp for SYBR Green I detection Magnesium concentration is too low run a titration to optimize concentration Insufficient or non specific product Verify product formation through gel electrop...

Page 39: ...icensor also grants to you a nonexclusive license to use the Materials solely in conjunction with your use of Software as licensed in this Agreement Modifications You may modify or change the Software...

Page 40: ...shall Licensor s liability to you whether in contract tort including negligence or otherwise exceed 250 00 This License Agreement is effective until terminated You may terminate this License Agreemen...

Page 41: ...may use only the medium appropriate for your single computer You may not use or install the other medium on another computer You may not loan rent lease or otherwise transfer the other medium to anoth...

Page 42: ...ria 01 25125 6800 Benelux 02 404 92 22 Denmark 45 70 13 00 30 Finland 010 802 220 France 0810 446 446 Germany 0800 603 1000 Italy 800 012575 Netherlands 020 547 2600 Spain 901 11 68 90 Sweden 08 506 4...

Page 43: ...com genomics Distributors For a list of worldwide distributors please visit our website www agilent com genomics This information is subject to change without notice Agilent Technologies Inc 2015 Pri...

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