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1100 Series WS MWS Reference Manual

Optimizing Performance

Autosamplers are more and more used in HPLC to improve the productivity in
the laboratories and the consistency and accuracy of analytical results.

The informations below will help you on how to optimize some parameters to
achieve best results for:

•

lowest carry-over for reliable quantitative data

•

Fast injection cycles for high throughput

•

Low delay volume for fast gradient

•

Precise injection volume

Summary of Contents for G1367A

Page 1: ...s1 Agilent 1100 Series Well plate Sampler Micro Well plate Sampler Reference Manual...

Page 2: ...erial in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnis...

Page 3: ...epairs requiring exchange of internal parts 5 Parts and Materials Detailed illustrations and lists for identification of parts and materials 6 Introduction to the Well plate Sampler An introduction to...

Page 4: ...4 1100 Series WS MWS Reference Manual...

Page 5: ...mended Plates and Closing Mat 34 List of Recommended Vials and Caps 36 Configure Well plate Types 38 Transporting the Sampler 41 2 Optimizing Performance Optimizing Performance 44 Optimization for Low...

Page 6: ...tus Indicator 59 Error Messages 60 Timeout 61 Shutdown 62 Remote Timeout 63 Synchronization Lost 64 Leak 65 Leak Sensor Open 66 Leak Sensor Short 67 Compensation Sensor Open 68 Compensation Sensor Sho...

Page 7: ...Turn ON and initialization steps 94 Errors which may occur during the turn ON and initialization process 95 Instrument logbook errors and step by step repair process 99 1 Fan error Main fan or Exhaust...

Page 8: ...s 118 Stator Face 119 Rotor Seal 120 Metering Seal and Plunger 122 Removing the Loop Capillary 124 Installing the Loop Capillary 126 Peristaltic Pump 128 Interface Board 129 Exchanging Internal Parts...

Page 9: ...ring drive motor and belt 142 Needle lock Motor and Belt 143 Removing the needle lock motor and belt 143 Installing the needle lock motor and belt 143 Main Fan 144 Removing the main fan 144 Installing...

Page 10: ...G1313 68711 only for G1367A 68A 176 Well plate Sampler Thermostat 177 Cable Overview 178 Analog Cables 180 Remote Cables 183 BCD Cables 188 Auxiliary Cable 191 CAN Cable 192 External Contact Cable 193...

Page 11: ...6 Position and Movement Sensors 217 Microtiter Plate Board MTP 218 Autosampler Specific Electronics 219 Firmware Description 223 Firmware Updates 224 Optional Interface Boards 225 BCD Board 225 LAN Bo...

Page 12: ...odule Screens of the Well plate Samplers Major keys on the Agilent 1100 Control Module 242 Screens available from the Analysis view 243 Screens available from the System view 259 9 Specifications Perf...

Page 13: ...Requirements 14 Unpacking the Sampler 17 Optimizing the Stack Configuration 20 Installing the Sampler 24 Installing a Thermostatted Sampler 26 Flow Connections to the Sampler 30 Sample Trays 32 List o...

Page 14: ...and a power plug for the line connections The two modules are connected by a control cable and both are turned on by the sampler module The thermostat power supply has two externally accessible fuses...

Page 15: ...trument requires an additional 25 cm 10 inches of space on either side for the circulation of air and approximately 8 cm 3 1 inches at the rear for electrical connections Ensure the autosampler is ins...

Page 16: ...avoid condensation Table 1 Physical Specifications sampler G1367A G1377A Type Specification Comments Weight 15 5 kg 34 2 lbs Dimensions height width depth 200 345 440 mm 8 13 5 17 inches Line voltage...

Page 17: ...al is damaged notify the carrier and save the shipping material for the carrier s inspection Delivery Checklist Ensure all parts and materials have been delivered with the autosampler For this compare...

Page 18: ...ntents G1367 68705 Description Quantity Part Number Capillary sampler column 380 mm 0 17 mm ID 1 01090 87306 96 well plate 0 5 ml PP pack of 10 1 5042 1386 Tubing assembly 1 5063 6527 Filter kit 1 506...

Page 19: ...g 1 5988 2999 Hex key 9 64 inch for injection valve screws 1 8710 0060 Wrenches 1 4 5 16 inch 2 8710 0510 Wrench 4 0 mm open end 1 8710 1534 Rheotool socket wrench 1 4 inch 1 8710 2391 Hex key 4 0 mm...

Page 20: ...by installing the following configuration Figure 1 and Figure 2 on page 21 show the configuration recommended for the sampler Figure 3 on page 22 and Figure 4 on page 23 show the configuration recomme...

Page 21: ...100 Series WS MWS Reference Manual 21 Figure 2 Recommended Stack Configuration Well plate Sampler Rear View GPIB or LAN to LC ChemStation Analog signal to recorder Remote cable CAN Bus cable AC power...

Page 22: ...WS Reference Manual 1 Installing the Sampler Figure 3 Recommended Stack Configuration Thermostatted Sampler Front View Autosampler ALS Thermostat Column Compartment Detector Control Module Pump Degass...

Page 23: ...eference Manual 23 Figure 4 Recommended Stack Configuration Thermostatted Sampler Rear View GPIB or LAN to LC ChemStation Analogsignal to recorder AC power Remote cable CAN bus cable Autosampler Therm...

Page 24: ...s OFF 9 Connect the power cable to the power connector at the rear of the sampler 10 Connect the CAN cable to the other Agilent 1100 modules 11 If a Agilent ChemStation is the controller connect eithe...

Page 25: ...of this process the status LED should be green Figure 5 Cable Connections Vial number output CAN cable to next module CAN cable to previous module Control of Thermostat NOTE The sampler is turned ON w...

Page 26: ...all the corrugated waste tube in the plastic port Preparation Locate bench space Provide power connections Unpack the sampler and the thermostat Parts required Sampler and thermostat Power cord for th...

Page 27: ...hat the cold airstream from the thermostat is correctly guided to the tray area of the well plate sampler 11 Re install the tray 12 Ensure the power switch on the front of the sampler is 0FF and the p...

Page 28: ...fan will turn ON and remove the vapor from the tray compartment After 1 2 minutes the sampler will start tile hardware initialisation process At the end of this process the status LED should be green...

Page 29: ...Installing the Sampler 1 1100 Series WS MWS Reference Manual 29 Figure 8 Connection at the rear of thermostatted Sampler Sampler Thermostat cable CAN bus AC Power...

Page 30: ...Drive the tube from the peristaltic flush pump to the solvent bottle in the solvent cabinet 6 Seat capillary see recommendations in Choice of Seat Capillary on page 54 Preparation Sampler is installed...

Page 31: ...Installing the Sampler 1 1100 Series WS MWS Reference Manual 31 Figure 9 Hydraulic Connections from pump to column to waste Corrugated tube Loop capillary waste tube...

Page 32: ...sample tray with sample well plates and vials as required 4 Slide the sample tray into the autosampler so that the rear of the sample tray is seated firmly against the rear of the sample tray area 5...

Page 33: ...rs G1367 60001 Tray for 2 plates and 10 x 2 ml vials G1313 44500 Tray for 100 x 2 ml vials Table 5 Trays for a thermostatted samplers G1367 60001 Tray for 2 plates and 10 x 2 ml vials G1329 60001 Tray...

Page 34: ...ent PN 96CorningV 8 12 14 3 300 No Agilent PN 96DeepAgilent31mm 8 12 31 5 1000 5042 6454 50 96DeepNunc31mm 8 12 31 5 1000 No Agilent PN 96DeepRitter41mm 8 12 41 2 800 No Agilent PN 96Greiner 8 12 14 3...

Page 35: ...ats with adhesive can give some contamination in the system The adhesive is soluble in most of the solvents used in HPLC WARNING In general do not use closing mats with adhesive The sampler has no pre...

Page 36: ...376 5183 4493 5183 4495 Table 8 SnapTop Vials Description Volume ml lOO Pack lOOO Pack lOO Pack silanized Clear glass 2 5182 0544 5183 4504 5183 4507 Clear glass write on spot 2 5182 0546 5183 4505 51...

Page 37: ...scription Septa 100 Pack Clear polypropyIene Clear PTFE red rubber 5182 0550 BIue polypropylene Clear PTFE red rubber 5182 3458 Green polypropylene Clear PTFE red rubber 5182 3457 Red polypropylene Cl...

Page 38: ...found on the List of recommended Plates and Closing Mat on page 34 you may configure a custom plate Measure the exact dimensions of the plate as marked below and enter the values in the plate configu...

Page 39: ...the plate up to 24 Volume Volume in I of a sample vessel A Row distance Distance in mm between the center of two rows B Column distance Distance in mm between the center of two columns C Plate length...

Page 40: ...r of the first hole A1 H Column shift Offset in mm to Y when the rows are not straight but staggered l Well diameter Diameter in mm of the well at least 4 mm J WeIl depth Distance in mm from the top o...

Page 41: ...boratory no special precautions are needed However if the autosampler needs to be shipped to another location via carrier ensure The transport assembly is in the park position Use the ChemStation or t...

Page 42: ...42 1100 Series WS MWS Reference Manual 1 Installing the Sampler...

Page 43: ...Sampler Reference Manual Agilent Technologies 2 Optimizing Performance Optimizing Performance 44 Optimization for Lowest Carry over 45 Fast Injection Cycle and Low Delay Volume 49 Precise Injection Vo...

Page 44: ...the productivity in the laboratories and the consistency and accuracy of analytical results The informations below will help you on how to optimize some parameters to achieve best results for lowest c...

Page 45: ...tion immediately after samples of high concentration carry over may become noticeable Cleaning the needle in the flush port or using the automated needle wash enables the carry over to be minimized an...

Page 46: ...hed in a flush port located behind the injection port on the sampling unit As soon the needle is in the flush port a peristaltic pump fills the flush port with fresh solvent during a defined time The...

Page 47: ...are used For example Outside wash of needle before injection 14 sec using flush port Injector program Draw x x y l from sample Needle wash as method Inject Wait equilibration time see text above Valve...

Page 48: ...an appropriate solvent Using an injector program and several wash vials can be used for cleaning In case the needle seat has got contaminated and carry over is significantly higher than expected the f...

Page 49: ...he sample has reached the column the injection valve is switched back to bypass and the next injection cycle starts but waits with switching to mainpass until the actual run is finished You gain the s...

Page 50: ...uld be set to Overlapped injection mode Wash time for needle outside set to 10 seconds Increase of draw and eject speed for large injection volumes Add at last run a blank if overlapped injection is u...

Page 51: ...Speed The speed at which the metering unit draws sample out of the vial may have an influence on the injection volume precision when using viscous samples If the draw speed is too high air bubbles may...

Page 52: ...w speed l Eject speed l Well Plate Sampler Default value 200 200 Minimum 10 10 Maximum 1000 1000 Micro Well Plate Sampler with 8 l loop capillary Default value 4 10 Minimum 0 7 0 7 Maximum 20 100 Micr...

Page 53: ...9 5 the Vespel seal may degrade faster leading to reduced seal lifetime Tefzel Seal For mobile phases with pH below 2 3 or above 9 5 or for conditions where the lifetime of the Vespel seal is drastica...

Page 54: ...seat G1377 87101 seat capillary choice of G1375 87317 100 m 150 mm G1375 87300 50 m 150 mm G1375 87317 100 m is the capillary preinstalled in micro well plate and thermostatted micro well plate sample...

Page 55: ...Overview of the Sampler s Indicators and Test Functions 56 Status Indicators 58 Error Messages 60 Maintenance Functions 88 Step Commands 90 Troubleshooting Guide for the Well Plate Sampler G1367A 93 T...

Page 56: ...sted actions to fix the problem are provided see Error Messages on page 60 Maintenance Functions The maintenance functions position the needle assembly the needle carrier the sample transport assembly...

Page 57: ...ns enable execution of each step of the sampling sequence individually The step functions are used primarily for troubleshooting and for verification of correct sampler operation after repair see Step...

Page 58: ...er The lower left indicates the power supply status the upper right indicates the sampler status Power Supply Indicator The power supply indicator is integrated into the main power switch When the ind...

Page 59: ...alysis run mode A yellow status indicator indicates a not ready condition The instrument is in a not ready state when it is waiting for a specific condition to be reached or completed for example fron...

Page 60: ...ch requires attention before the analysis can be continued for example repair exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is...

Page 61: ...ysis was completed successfully and the timeout function switched off the pump as requested A not ready condition was present during a sequence or multiple injection run for a period longer than the t...

Page 62: ...able Causes Leak detected in another Agilent 1100 module with a CAN connection to the system Error detected in an external instrument with a remote connection to the system The degasser failed to gene...

Page 63: ...sis If a not ready condition is still present on the remote line after one minute the error message is generated Probable Causes Not ready condition in one of the instruments connected to the remote l...

Page 64: ...he system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable Causes CAN cable disconnected Defective CAN cable...

Page 65: ...This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the MTP board Probable Causes Loose fittings Broken capillary Leaking rotor seal or needle seat Defective m...

Page 66: ...ependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error mes...

Page 67: ...cuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the curren...

Page 68: ...t The resistance across the temperature compensation sensor NTC on the MTP board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temp...

Page 69: ...cuit The resistance across the temperature compensation sensor NTC on the MTP board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient t...

Page 70: ...or on the fan shaft is used by the MTP board to monitor the fan speed If the fan speed falls below 2 revolutions second for longer than 5 seconds the error message is generated Probable Causes Fan cab...

Page 71: ...l sensor on the fan shaft is used by the WPS board to monitor the fan speed If the fan speed falls below a certain value the error message is generated and the well plate sampler shuts down Probable C...

Page 72: ...Functions Front Door Error The front door and or the SLS board are damaged Probable Causes The sensor on the SLS board is defective The door is bent or the magnet is misplaced broken Suggested Action...

Page 73: ...S Reference Manual 73 Side Door Error The side door and or the MTP board are damaged Probable Causes The door is bent or the magnet is misplaced broken The sensor on the MTP board is defective Suggest...

Page 74: ...pper motors If the processor does not receive the correct position information from the encoders within the time window the error message is generated See figure for axes identification Arm Movement 0...

Page 75: ...valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the bypass position or if the microswitch does not close the error message is generated P...

Page 76: ...alve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the mainpass position or if the microswitch does not close the error message is generated...

Page 77: ...rd The sensors detect the successful completion of the needle lock movement If the needle lock fails to reach the end point or if the sensors fail to recognize the needle lock movement the error messa...

Page 78: ...encoder fails to recognize the needle carrier movement the error message is generated Probable Causes Bad sample transport sampling unit alignment Bent needle Missing needle Blocked seat Defective pos...

Page 79: ...Sample Transport Assembly failed to move correctly Probable Causes Defective Z motor Vial pusher blocked Bad needle carrier positioning in X or Theta Defective vial pusher sensor Defective MTP main bo...

Page 80: ...oder behind the vial pusher If no vial is present the encoder detects an error and the message missing vial is generated Probable Causes No vial in the position defined in the method or sequence Defec...

Page 81: ...ovements is not successful or is not detected the error message is generated Probable Causes Side door not installed correctly Sample transport sampling unit not aligned correctly Mechanical obstructi...

Page 82: ...he home position of the plunger If the plunger fails to move to the home position or if the sensor fails to recognize the plunger position the error message is generated Probable Causes Dirty or defec...

Page 83: ...y each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the ti...

Page 84: ...ition does not exist in the current sample tray configuration the error message is generated Probable Causes Incorrect tray installed Incorrect tray definition Incorrect vial positions defined in the...

Page 85: ...ed The current on the motor is used by the MTP board to monitor the speed of the peristaltic pump motor If the current falls below a certain value the error message is generated Probable Causes Defect...

Page 86: ...e vessel If the needle fails to reach the end point the sensor fails to recognize the needle movement and the error message is generated Probable Causes Bad vessel definition in the plate configuratio...

Page 87: ...e moves up Possible causes Closing mat to rigid thick Bad X or Theta positioning and the needle sticks into the wall between two holes Defective encoder on the needle carrier assembly Suggested Action...

Page 88: ...e maintenance functions moves the arm assembly in a specific position in order to enables easy access for maintenance operations Home Position The home position function moves the arm to the right sid...

Page 89: ...r when you exchange The sample transport The sampling unit The MTP main board The well plate sampler tray Table 15 Maintenance positions Function Arm position in X Arm position in Theta Arm Position i...

Page 90: ...play In the Control Module the step commands can be accessed from the pull down menu in the well plate sampler Test Table 16 Step Commands Step Action Comments Bypass Switches injection valve to the b...

Page 91: ...the needle arm into the seat Mainpass Switches the injection valve to the mainpass position Needle Up Mainpass Lifts the needle arm to the upper position and Switches the injection valve to the mainpa...

Page 92: ...needle drive motor Mainpass Valve not connected Defective injection valve Needle Up Mainpass Blockage in the sample loop or needle no solvent flow Defective or dirty sensor on the sampling unit flex...

Page 93: ...epage http lsbu marketing agilent com start start asp Products Injection system G1367A Technical note Gather information about the problem Instrument serial number Instrument firmware revision and use...

Page 94: ...t 20 seconds from turn on The status indicator remains yellow until the initialization process is complete The user interface indicates initializing during this period 4 The vapor blowout period begin...

Page 95: ...r supply defective Suggested actions Turn the WPS off Disconnect the power supply from the main board see if power indicator becomes green when the WPS is turned on If yes change the main board G1367...

Page 96: ...ptom 1 Main board initialization fails Status indicator remains off but the rest of the turn on initialization process is successful Possible causes The heel of the light pipe is not positioned correc...

Page 97: ...cover open message Suggested actions Close the front cover If the front cover open message is still displayed Make sure the front cover magnet is close enough to the magnet sensor Check the ribbon ca...

Page 98: ...hing which may have been done just before the initialization failure occurred To date we have seen initialization errors occur for a variety of reasons As an overall troubleshooting plan follow the su...

Page 99: ...n 3160 4097 Replace the main board G1367 69500 2 Initialization error Upgrade the firmware revision to A 04 14 or higher and the ChemStation revision to A 08 04 or higher Check the sample transport co...

Page 100: ...onnector on the main board Check the connector on the sampling unit Change the rheodyne valve 0101 0921 Change the sampling unit G1367 60008 Change the main board G1367 69500 5 Needle lock error Check...

Page 101: ...o position 1 Turn on the system Perform an auto alignment Turn off the system Move the 8 bit configuration dip switches 1 2 8 at the rear of the instrument to position 0 Turn on the system Check the c...

Page 102: ...alignment in the pusher Check if the seat is not blocked with any parts or material crystals glass Perform an auto alignment Check the connector from the needle carrier to the sample transport unit C...

Page 103: ...auto alignment Check the connector from the needle carrier to the sample transport unit Check the sample transport connectors on the main board sample transport Check the connector on the bottom of t...

Page 104: ...far forward as possible and centered in the vial pusher Upgrade the firmware revision to A 04 14 or higher and the ChemStation revision to A 08 04 or higher Perform an auto alignment without any plat...

Page 105: ...ate Sampler Micro Well plate Sampler Reference Manual Agilent Technologies 4 Repairing the Sampler Introduction into Repairing the Sampler 106 Overview of Main Repair Procedures 108 Simple Repairs 109...

Page 106: ...of these parts requires removing the sampler from the stack removing the covers and disassembling the sampler Cleaning the Autosampler The sampler covers should be kept clean Cleaning should be done w...

Page 107: ...ly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground CAUTION Electronic...

Page 108: ...al 4 Repairing the Sampler Overview of Main Repair Procedures Figure 18 Main Assemblies Peristaltic pump Injection valve Analytical head Needle Transport assembly MTP board Power supply Needle seat SL...

Page 109: ...needle seat assembly When the seat shows indication of damage or blockage See Exchange the Needle Seat Assembly G1367 87101 on the G1367A 68A Samplers on page 116 Exchanging the stator face When the...

Page 110: ...with the loop capillary from the needle carrier When required When the needle is visibly damaged When the needle is blocked Tools required Two 1 4 inch 5 16 inch wrenches 8710 0510 supplied in accesso...

Page 111: ...ce Manual 111 9 Attach the 5 16 inch wrench to hold position at the needle assembly Use the 4 mm wrench to loosen the fitting of the loop capillary 10 Pull the loop capillary out from the needle assem...

Page 112: ...e needle assy until the stop 9 Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pus...

Page 113: ...ace the plate tray in the tray base Re install the side door and close the front door 12 In the user interface close the Change Needle Seat function and exit the maintenance mode NOTE The needle must...

Page 114: ...he silicon safety tube over the needle 5 Pinch the holder clamp pull back and remove the needle assembly from the needle carrier 6 Unplug the flex board on the sample transport 7 Unscrew the three hol...

Page 115: ...ral directions to see that it is aligned in the center of the needle pusher 6 Remove the silicon safety tube from the needle 7 Replace the plate tray in the tray base 8 Re install the side door and cl...

Page 116: ...9 In the user interface close the Change Needle Seat function and exit the maintenance mode When required When the seat is visibly damaged When the seat capillary is blocked Tools required 1 4 inch 5...

Page 117: ...on and exit the maintenance mode When required When the seat is visibly damaged When the seat capillary is blocked Tools required 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4 mm ope...

Page 118: ...8710 1534 supplied in accessory kit Flat screwdriver Parts required G1367 87101 Needle Seat assy 0 17 mm ID 2 3 l for G1367 68A G1377 87101 Needle Seat without capillary for G1377 78A G1375 87317 Sea...

Page 119: ...er NOTE This procedure is only for the injection valve on the G1367A 68A samplers The micro injection valve on the G1377A 78A samplers has no ceramic stator face When required When poor injection volu...

Page 120: ...e holes on the stator head When required When poor injection volume reproducibility When leaking injection valve Tools required 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 9 64 inch...

Page 121: ...1 9 Install this stator head face assy on the injection valve Tighten the screws alternately with the 9 64 inch wrench until the stator head is secure 10 Reconnect all the capillaries to the injection...

Page 122: ...ter is removed When required When poor injection volume reproducibility When leaking metering device Tools required 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4 mm open end wrench 8...

Page 123: ...ing screws in place and reinstall the analytical head to the sampling unit 4 Tighten alternately the two fixing screws with the 4 mm hex key 5 Connect the two capillaries to the analytical head Use a...

Page 124: ...illary blocked Capillary broken Tools required Two 1 4 inch 5 16 inch wrenches 8710 0510 supplied in accessory kit Parts required G1367 87300 Loop capillary injection volume up to 100 l for the G1367A...

Page 125: ...lamp pull back and remove the needle assembly with the loop capillary from the needle carrier 11 Attach the 5 16 inch wrench to hold position at the needle assembly Use the 4 mm wrench to loosen the f...

Page 126: ...le pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher When required Capillary blocked Capillary broken Tools required Two 1 4...

Page 127: ...e Manual 127 10 Remove the silicon safety tube from the needle 11 Replace the plate tray in the tray base Re install the side door and close the front door 12 In the user interface close the Change Lo...

Page 128: ...e 5 Connect the wash port tubing to the upper tubing of the new pump use sandpaper to get a good grip on the tubing 6 Connect the tubing coming from the solvent bottle to the lower tubing of the new p...

Page 129: ...emove the cover 4 Carefully insert the new interface board into the holding rails and push the board into the slot Make sure the board plugs into the socket correctly 5 Reconnect all cables to the new...

Page 130: ...er cord WARNING When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the...

Page 131: ...ss the front door into the slots in the top cover Light protection kit installation 1 Remove the transparent front door and install the dark one 2 Slide the transparent window out of the side panel 3...

Page 132: ...he top foam Press the foam firmly into place 2 Engage the slot on the top plate onto the side plate 3 Fix the top plate in place with the three screws 4 Engage the top cover into the bottom cover 5 Lo...

Page 133: ...e the new transport assembly into the sampler 2 Ensure the transport assembly is seated firmly 3 Reconnect the loop capillary to the analytical head Use a 1 4 inch wrench if you reconnect a SST capill...

Page 134: ...ch if you remove a fused silica capillary 7 Slide the sampling unit and tray base back to disengage the sampling unit connector 8 Lift the sampling unit and tray base approximately 10 cm Slide the lea...

Page 135: ...port 1 and the one going to the column compartment on port 6 of the injection valve Use a 1 4 inch wrench if you reconnect a SST capillary or a 4 mm wrench if you reconnect a fused silica capillary 8...

Page 136: ...nit 2 Install and tighten alternately the two hex keys which fix the injection valve assembly 3 Alternately tighten the two fixing screws with the 4 mm hex key 4 Connect the two capillaries on the ana...

Page 137: ...ng unit see Removing the sampling unit on page 134 7 Remove the air channel 8 Remove the peristaltic pump see Peristaltic Pump on page 128 9 Remove the base plate of the pump with a small flat head sc...

Page 138: ...air channel 6 Assemble the peristaltic pump see Peristaltic Pump on page 128 7 Install the sampling unit see Installing the sampling unit on page 135 8 Install the sample transport assembly see Insta...

Page 139: ...ort assembly see Removing the sample transport on page 133 6 Remove the sampling unit see Removing the sampling unit on page 134 7 Disconnect the valve cable connector from the SUD board Valve Assy 8...

Page 140: ...ws which fix the injection valve assembly 4 Install the sampling unit see Installing the sampling unit on page 135 5 Install the sample transport see Installing the sample transport on page 133 6 Inst...

Page 141: ...rom the tray base 5 Remove the transport assembly see Removing the sample transport on page 133 6 Remove the sampling unit see Removing the sampling unit on page 134 7 Disconnect the motor connector f...

Page 142: ...seated correctly over the gear and motor shaft 4 Install the sampling unit see Installing the sampling unit on page 135 5 Install the sample transport see Installing the sample transport on page 133 6...

Page 143: ...emove the motor Installing the needle lock motor and belt 1 Feed the motor cable through to the SUD board Connect the cable to the connector labelled Needle M 2 Secure the motor in place with the four...

Page 144: ...of the foam Installing the main fan 1 Install the new fan and connect the fan connector to the MTP main board 2 Install the sample transport see Installing the sample transport on page 133 3 Install...

Page 145: ...ain board on page 146 7 Remove the exhaust fan Installing the exhaust fan 1 Install the exhaust fan 2 Install the MTP main board see Installing the MTP main board on page 147 3 Connect the fan connect...

Page 146: ...crews at the back of the module 7 Remove the M4 screw at the back of the module 8 Disconnect all connectors on the MTP board 9 Slide the MTP main board out of the sampler When required When defective...

Page 147: ...page 133 6 Install the top cover top plate and foam see Top Cover and Foam on page 132 7 Replace the plate tray in the tray base 8 Turn ON the sampler close the front door the system will reset autom...

Page 148: ...e correct serial number for this module Entering the Serial Number using the ChemStation Module serial numbers are entered by typing specific commands into the command line at the bottom of the main u...

Page 149: ...tray base 5 Remove the transport assembly see Removing the sample transport on page 133 6 Remove the sampling unit see Removing the sampling unit on page 134 7 Disconnect all connectors from the SUD b...

Page 150: ...tors to the new board 4 Install the sampling unit see Installing the sampling unit on page 135 5 Install the sample transport see Installing the sample transport on page 133 6 Install the top cover to...

Page 151: ...ard and unscrew the 2 holding screws 9 Remove the SLS board from the inside of the module Installing the SLS board 1 Install the new SLS board and the SLS board cover 2 Install the sampling unit see I...

Page 152: ...oard on page 146 8 Remove the fan see Removing the main fan on page 144 9 Remove the bottom foam 10 Remove the exhaust fan see Removing the exhaust fan on page 145 11 Remove the power switch light pip...

Page 153: ...ge 145 6 Install the MTP board see Installing the MTP main board on page 147 7 Install the main fan see Installing the main fan on page 144 8 Install the sampling unit see Installing the sampling unit...

Page 154: ...sor Installing the leak sensor 1 Feed the cable of the new sensor through the base of the sampling unit to the SUD board 2 Connect the connector to the connector labelled leak sensor 3 Install the sam...

Page 155: ...vious installed one To upgrade the autosampler firmware the following steps have to be performed 1 Load the firmware into the autosampler see the help system of your user interface For control module...

Page 156: ...156 1100 Series WS MWS Reference Manual 4 Repairing the Sampler...

Page 157: ...tical Head Assembly 165 Injection Valve Assembly 167 Sheet Metal Kit 169 Cover Parts 170 Foam Parts 171 Power and Status Light Pipes 172 Leak System Parts 173 Well plate Sampler Accessory Kit G1367 68...

Page 158: ...158 1100 Series WS MWS Reference Manual 5 Parts and Materials Sampler Main Assemblies Figure 19 Well plate Sampler Main Assemblies 6 7 5 3 2 9 13 1 12 8 10 4 11...

Page 159: ...or G1377 78A 0101 0921 0101 1050 7 Needle Seat assy for G1367 68A Needle Seat assy for G1377 78A without capillary Seat cap 0 10 mm ID 1 2 l for G1377 87101 Needle Seat Seat cap 0 05 mm ID 0 3 l for G...

Page 160: ...tes 10 2 ml vials G1367 60001 2 Tray for 100 2 ml vials thermostattable G1329 60001 3 Tray for 100 2 ml vials G1313 44500 4 Screws for springs 0515 0866 5 Spring G1313 09101 6 Spring stud 0570 1574 7...

Page 161: ...ent PN 96Agilent 8 12 14 3 400 5042 1386 5042 1385 10 120 96CappedAgilent 8 12 47 1 300 5065 4402 1 96Corning 8 12 14 3 300 No Agilent PN 96CorningV 8 12 14 3 300 No Agilent PN 96DeepAgilent31mm 8 12...

Page 162: ...162 1100 Series WS MWS Reference Manual 5 Parts and Materials Sampling Unit Assembly Figure 21 Well plate sampler sampling unit 1 2 3 4 6 5 16 9 12 13 11 14 15 10 7 8...

Page 163: ...00 2086 7 Analytical Head assembly 100 l for G1367 68A Analytical Head assembly 40 l for G1377 78A G1367 60003 G1377 60013 8 Peristaltic pump includes tubing 5065 4445 9 Inj Valve Anal Head cap 160 mm...

Page 164: ...5 Parts and Materials Stepper motor peristaltic pump not visible 5065 4409 Motor holder not visible G1367 42304 Plate peristaltic pump not visible G1367 44100 Table 22 Well plate Sampler sampling unit...

Page 165: ...ering seal pack of 2 for G1367 68A 5063 6589 6 Head body 01078 27710 Screw M5 60 mm lg for mounting of assembly 0515 2118 Table 24 Micro analytical head assembly 40 l for G1377 78A Item Description Pa...

Page 166: ...166 1100 Series WS MWS Reference Manual 5 Parts and Materials Figure 22 Analytical Head Assembly 100 l or 40 l 2 4 5 6 1 3...

Page 167: ...seal Vespel 0100 1853 3 Rotor seal Tefzel 0100 1849 4 Stator face 0100 1851 5 Stator head 0100 1850 6 Stator screws 1535 4857 Table 26 Micro Injection Valve Assembly The Micro injection valve assembl...

Page 168: ...168 1100 Series WS MWS Reference Manual 5 Parts and Materials Figure 23 Injection Valve Assembly 1 2 4 3 6 5...

Page 169: ...eries WS MWS Reference Manual 169 Sheet Metal Kit Table 27 Sheet Metal Item Description Part Number 1 Slot cover 5001 3772 2 Screw cover 5022 2112 3 Well plate sampler sheet metal kit G1367 68701 Figu...

Page 170: ...er Parts Table 28 Covers Item Description Part Number 1 Cabinet kit includes base side panels top and front cover 5065 4446 Name plate for Agilent 1100 Series 5042 1381 Light protection kit includes d...

Page 171: ...S MWS Reference Manual 171 Foam Parts Table 29 Foam Parts Item Description Part Number Foam kit includes items 2 and 3 5064 8248 1 Board guides 5041 8395 2 Top foam Order foam kit 3 Bottom foam Order...

Page 172: ...d Status Light Pipes Table 30 Power and Status Light Pipes Item Description Part Number 1 Light pipe power switch 5041 8382 2 Power switch button 5041 8381 3 Light pipe status lamp 5041 8384 4 Power s...

Page 173: ...nce Manual 173 Leak System Parts Table 31 Leak System Parts Item Description Part Number 1 Leak sensor 5061 3356 2 Leak plane G1313 44501 3 Leak tubing 120 mm reorder gives 5 m 5062 2463 4 Leak funnel...

Page 174: ...1 5064 8240 CAN cable 1 m 1 5181 1519 Vials screw cap 100 pk 1 5182 0716 Blue screw caps 100 pk 1 5182 0717 Valve catalog 1 5988 2999 Hex key 9 64 inch for injection valve screws 1 8710 0060 Wrenches...

Page 175: ...1 5182 0717 Valve catalog 1 5988 2999 Hex key 9 64 inch for injection valve screws 1 8710 0060 Wrenches 1 4 5 16 inch 2 8710 0510 Wrench 4 0 mm open end 1 8710 1534 Rheotool socket wrench 1 4 inch 1...

Page 176: ...nual 5 Parts and Materials Multi Draw Kit G1313 68711 only for G1367A 68A Table 34 Multi Draw Kit Item Description Part Number 1 Seat capillary 500 l 0 5 mm id G1313 87307 2 Seat capillary 1500 l 0 9...

Page 177: ...Materials 5 1100 Series WS MWS Reference Manual 177 Well plate Sampler Thermostat Table 35 WPS Thermostat Item Description Part Number 1 WPS thermostat exchange assembly G1330 69020 Figure 29 WPS The...

Page 178: ...Remote cables 3390 integrator 01046 60203 3392 3 integrators 01046 60206 3394 integrator 01046 60210 3396A Series I integrator 03394 60600 3396 Series II 3395A integrator see page 185 3396 Series III...

Page 179: ...terface board to general purpose G1103 61611 GPIB cable Agilent 1100 module to ChemStation 1 m 10833A Agilent 1100 module to ChemStation 2 m 10833B RS 232 cable Agilent 1100 module to a computer This...

Page 180: ...o Agilent 1100 Series modules The other end depends on the instrument to which connection is being made Table 37 Agilent 1100 to 3390 2 3 Integrators Connector 01040 60101 Pin 3390 2 3 Pin Agilent 110...

Page 181: ...t 1100 to 3394 6 Integrators Connector 35900 60750 Pin 3394 6 Pin Agilent 1100 Signal Name 1 Not connected 2 Shield Analog 3 Center Analog Table 39 Agilent 1100 to BNC Connector Connector 8120 1840 Pi...

Page 182: ...1100 Series WS MWS Reference Manual 5 Parts and Materials Table 40 Agilent 1100 to General Purpose Connector 01046 60105 Pin 3394 6 Pin Agilent 1100 Signal Name 1 Not connected 2 Black Analog 3 Red An...

Page 183: ...1100 Series modules The other end depends on the instrument to be connected to Table 41 Agilent 1100 to 3390 Integrators Connector 01046 60203 Pin 3390 Pin Agilent 1100 Signal Name Active TTL 2 1 Whit...

Page 184: ...ow NC 5 Pink Not connected NC 6 Yellow Power on High 9 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low Table 43 Agilent 1100 to 3394 Integrators Connector 01046 60210 Pin 3394 Pin Agi...

Page 185: ...OTE START and STOP are connected via diodes to pin 3 of the 3394 connector Table 44 Agilent 1100 to 3396A Integrators Connector 03394 60600 Pin 3394 Pin Agilent 1100 Signal Name Active TTL 9 1 White D...

Page 186: ...6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Table 46 Agilent 1100 to HP 1050 HP 1046A or Agilent 35900 A D Converters Connector 5061...

Page 187: ...3 Gray Start Low 7 4 Blue Shut down Low 8 5 Pink Not connected NC 6 Yellow Power on High 3 7 Red Ready High 6 8 Green Stop Low NC 9 Black Start request Low Table 48 Agilent 1100 to General Purpose Con...

Page 188: ...depends on the instrument to be connected to NOTE The BCD output for the well plate sampler does not work with the integrators 3392 3 6 Table 49 Agilent 1100 to 3392 3 Integrators Connector 18584 605...

Page 189: ...ual 189 Table 50 Agilent 1100 to 3396 Integrators Connector 03396 60560 Pin 3392 3 Pin Agilent 1100 Signal Name BCD Digit 1 1 BCD 5 20 2 2 BCD 7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCD 0 1 6 6 BCD 3 8 7...

Page 190: ...1600 Wire Color Pin Agilent 1100 Signal Name BCD Digit Green 1 BCD 5 20 Violet 2 BCD 7 80 Blue 3 BCD 6 40 Yellow 4 BCD 4 10 Black 5 BCD 0 1 Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digita...

Page 191: ...modular plug to be connected to the Agilent 1100 Series vacuum degasser The other end is for general purpose Table 52 Agilent 1100 Series Degasser to general purposes Connector G1322 81600 Color Pin...

Page 192: ...le Both ends of this cable provide a modular plug to be connected to Agilent 1100 Series module s CAN bus connectors Table 53 Agilent 1100 module to module 0 5m lg 5181 1516 Agilent 1100 module to mod...

Page 193: ...able 54 Agilent 1100 Series Interface Board to general purposes Connector G1103 61611 Color Pin Agilent 1100 Signal Name White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EX...

Page 194: ...one adapter Use the cable and adapter to connect Agilent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent 1100 module to PC RS 232 Cable Kit 34398A Table 55...

Page 195: ...ual 195 LAN Cables Recommended Cables For point to point connection not using a network hub use a twisted pair cross over LAN cable P N 5183 4649 10 feet long For standard network connections using a...

Page 196: ...196 1100 Series WS MWS Reference Manual 5 Parts and Materials...

Page 197: ...anual Agilent Technologies 6 Introduction to the Well plate Sampler Introduction to the Well plate Sampler 198 Sampling Sequence 200 Sampling Unit 204 Needle Sample Transport Assembly 207 Advanced Ope...

Page 198: ...able sample tray for this instrument holds 2 well plates shallow or deepwell and additional 10 x 2 0 ml vials The format of the well plates can be up to 384 wells per plate on a footprint of 128x86 mm...

Page 199: ...mn directly During injection and analysis the valve unit directs the flow through the well plate sampler which ensures that all of the sample is injected onto the column and that the metering unit and...

Page 200: ...ll plate sampler by the injection valve during the sampling sequence The needle moves to the desired sample vial position and is lowered into the sample liquid in the vial to allow the metering device...

Page 201: ...s to the desired sample vial position 5 The needle lowers into the vial 6 The metering device draws the preset sample volume 7 The needle lifts out of the vial 8 The needle is then moved onto the seat...

Page 202: ...ion the mobile phase flows through the well plate sampler metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over When th...

Page 203: ...needle can be washed in a flush port located behind the injector port on the sampling unit As soon the needle is on the flush port a peristaltic pump delivers some solvent during a defined time to cle...

Page 204: ...unger against the tension of the spring into the analytical head The base of the plunger sits on the large bearing of the drive nut which ensures the plunger is always centered A ceramic ring guides t...

Page 205: ...the stepper motor to the injection valve Two microswitches monitor switching of the valve bypass and mainpass end positions The injection valve has a ceramic stator Vespel rotor seal Tefzel seal avai...

Page 206: ...by a separate flex tube to a waste bottle Needle Lock A needle lock is used to support the needle carrier in its function making a firm seal of the needle in its seat The needle lock arm is driven by...

Page 207: ...ials needle wash position and the needle seat position The active movable axes are the X axis the Z axis and the theta axis the vial plate pusher is an additional passive axis All axes are stepper mot...

Page 208: ...ase the number of different trays It allows to read the revision and other data tags of the needle sample transport assembly and sampling unit Complex flex boards make the electrical connection to the...

Page 209: ...the Well plate Sampler 6 1100 Series WS MWS Reference Manual 209 Advanced Operating Modes Figure 35 Needle Sample Transport Assembly Theta axis X axis Z axis Needle assembly Needle carrier Reflective...

Page 210: ...sh mode allows also the flushing of the outer surface of the needle This results in an additional decrease of sample carry over Duration of the procedure is setable Overlap injection cycle Overlapped...

Page 211: ...cheduled EMF Counters The autosampler provides four EMF counters Each counter increments with autosampler use and can be assigned a maximum limit which provides visual feedback in the user interface w...

Page 212: ...operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument pe...

Page 213: ...pliance with safety or EMC regulations Figure 36 Well plate Sampler Electrical Connections DC Out RS232C CAN bus Remote Vial number output Relay contacts CAN cable to next module CAN cable to previous...

Page 214: ...214 1100 Series WS MWS Reference Manual 6 Introduction to the Well plate Sampler...

Page 215: ...nt Technologies 7 Theory of Operation Autosampler Control and Electronics 216 Position and Movement Sensors 217 Microtiter Plate Board MTP 218 Firmware Description 223 Optional Interface Boards 225 In...

Page 216: ...late Board MTP controls the vial transport mechanism sampling needle metering unit and high speed injection valve These devices are controlled by a versatile electronics design based upon a 68000 fami...

Page 217: ...ensed Reflection Sensor 9 Vial tray identification Reflection Sensor 4 Transport assembly Initialization Table 59 Sampling Unit Flex Board Sensor Type Number of Sensors Position Movement Sensed IR lig...

Page 218: ...ess for the communication baud rate for RS 232 transfer and so on Also the ASIC controls and drives module specific functions and reads static status signals Leak Converter Solvent leaking from the au...

Page 219: ...evolution This fan status signal is used for diagnostics Onboard Battery An onboard lithium battery buffers the electronic memories when the module is turned off For safety information on lithium batt...

Page 220: ...and the up down signal are used in the FPGA to provide instantaneous stepper motor commutation with respect to the motor rotor position Wiring between the well plate sampler main board ASM and the mot...

Page 221: ...nsing of the valve motor is done by two microswitches Two reflection light sensors are used to detect the end positions of the needle lock device One photo sensor is required to detect the home positi...

Page 222: ...d by an electric magnet Unlocking the front door is done by pushing the button on the right side or switching the power off on Figure 39 Well plate sampler block diagram FPGA theta Z Axis X Axis Meter...

Page 223: ...re the complete communication capabilities CAN LAN and RS 232C memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilitie...

Page 224: ...aming conventions are xxxx vvv DLB where xxxx is the product number e g 1367A for the well plate sampler and vvv is the revision number for example 380 is revision 3 80 For instructions refer to your...

Page 225: ...ries well plate sampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused There is a general purpose cable available...

Page 226: ...gram BCD Board Vial 1 Vial 10 Pos P2 P24 Vial 985 Plate 2 Pos P2 A1 Vial 601 Pos P2 B1 Vial 625 Pos P1 H12 Vial 297 Pos P1 A1 Vial 201 Pos P1 B1 Vial 213 Plate 1 Processor interface Board identificati...

Page 227: ...s P N G1530 61480 8 m long NOTE One board is required per Agilent 1100 stack It is recommended to add the LAN board to the detector with highest data rate NOTE The LAN board can only be used together...

Page 228: ...100 Series Interfaces Interface Type Pumps Autosampler Well plate Sampler DA Detector MW Detector FL Detector VW Detector RI Detector Thermostatted Column Compartment Vacuum Degasse r CAN Yes Yes Yes...

Page 229: ...address of your module The switches are preset to a default address and recognized by the operating software from Agilent Technologies CAN Interface The CAN is an intermodule communication interface...

Page 230: ...y powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and option...

Page 231: ...system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready sta...

Page 232: ...ion 9 In RI Figure 43 RS 232 Cable Table 68 RS 232C Connection Table continued Pin Direction Function Instrument DCD RX TX DTR GND DSR RTS CTS RI DCD RX TX DTR GND DSR RTS CTS RI PC 1 2 3 4 5 6 7 8 9...

Page 233: ...en completed the instrument must be powered up again in order to store the values in the non volatile memory In the non volatile memory the parameters are kept regardless of whether you turn the instr...

Page 234: ...ng Your Agilent ChemStation System handbook Default GPIB address is set to the following addresses where 0 means that the switch is down and 1 means that the switch is up Table 70 Default Addresses fo...

Page 235: ...es in the non volatile memory Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch...

Page 236: ...up will allow for normal operation If you use the following switch settings and power the instrument up again a forced cold start has been completed To return to normal operation set switches back to...

Page 237: ...itions other than being both up will allow for normal operation If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident part that is it...

Page 238: ...in the detectors The line voltage can vary in a range from 100 240 volts AC 10 and needs no manual setting To disconnect the instrument from line unplug the power cord The power supply still uses some...

Page 239: ...output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrumen...

Page 240: ...240 1100 Series WS MWS Reference Manual 7 Theory of Operation...

Page 241: ...Well plate Sampler Reference Manual Agilent Technologies 8 Control Module Screens of the Well plate Samplers Major keys on the Agilent 1100 Control Module 242 Screens available from the Analysis view...

Page 242: ...ce Done If available Activate settings of current screen On Off Switch on individual Instrument s or complete System Start Start a location range or sequence Plot View online signals Views Change betw...

Page 243: ...ence Manual 243 Screens available from the Analysis view Analysis view This is the wake up screen if the Agilent 1100 Series well plate samplers or micro well plate samplers are the only configured Ag...

Page 244: ...change time settings Print Screen gives access to the print screen About tells you the current firmware revision and the serial of your control module Setup view leads you to analysis view configurati...

Page 245: ...ction keys Use function keys F7 F8 Remove Add to move the highlighted module Changes must be activated with Done F6 Here e g the binary pump parameters are shown on the display as well The number of p...

Page 246: ...Screens of the Well plate Samplers The m key gives access to a context sensitive menu related to the module that is highlighted by the cursor like in this case the WPS With the F1 key Settings you op...

Page 247: ...toggle between Standard Injection with without needle wash and Injector Program There are different sets of parameters available through the F1 5 navigation keys for optimizing the operation of the W...

Page 248: ...f the Well plate Samplers Use the m key for the context sensitive menu The Status command pulls up a module specific screen displaying details of injections and the well plate trays Reset will initial...

Page 249: ...an enter WPS set points for the Draw Speed Eject Speed and Draw Position Offset Changes must be acknowledged with F6 Done By selecting More Needle Wash on the pull down menu you can specify the time p...

Page 250: ...Equilibration Time after drawing and before injecting and you can enable disable the Vial Well Bottom Sensing Changes must be acknowledged with F6 Done By selecting More High Throughput on the pull d...

Page 251: ...ed for the calculation of the flushout time of the sampler depending on flow rate and internal volume of the sampler By selecting More Sample Flush Out on the pull down menu you can enter the Sample F...

Page 252: ...dit the selected entry or F6 key Delete to remove the selected highlighted entry Edit each entry line according to your needs by entering a time for a Setting and by selecting a Setting with the help...

Page 253: ...Inj Program you can change the settings for the analog outputs Press the F7 key Enter to add program events Use the F6 key Done to view the entered lines of the timetable Use the m key for the context...

Page 254: ...ostat With the F4 key Thermostat you can set the well plate sampler thermostat temperature Changes must be acknowledged with F6 Done Settings Run times With the F5 key Runtimes you can change the stop...

Page 255: ...5 key Views and select Status This is an example if an Agilent 1100 WPS is configured stand alone Signal plot Press F6 key Plot to enter the plot screen available also from all other screens Here you...

Page 256: ...3 Additional signals could be also pressure or temperature signals from other modules Use the Right Left selection keys to switch between Available Signals and Selected Signals Use the F8 key Move to...

Page 257: ...y Method to view the parameters in a method Use the F8 key Save As to save the method in the mdodule s The PC Card key is only active when a PCMCIA card is inserted in the control module Use F2 key PC...

Page 258: ...lection keys to switch between PC Card and Instrument window Use the Up Down selection keys to select the method Use F7 F8 Copy to copy a selected method from one window to the other one Use F6 Delete...

Page 259: ...mplers 8 1100 Series WS MWS Reference Manual 259 Screens available from the System view System view Use the Esc key until you receive Views on the F5 key Choose System from the pull down menu This scr...

Page 260: ...amplers System Control Use the F1 key Control to select the well plate Sampler Here you receive information about the not Ready conditions if needed F7 Reset does a re initialization of the WPS Use th...

Page 261: ...ews on the F5 key in the Analysis Screen Choose System from the pull down menu Use the F2 key Configure to select the WPS Here you define further special set points for the WPS operation Changes must...

Page 262: ...ction keys F7 F8 Add Remove allows you to move configurations from available Plate Formats to the Active Plates and vice versa Press F2 Delete Def to delete a selected definition Press F3 Rotate Plate...

Page 263: ...nfiguration screen allows you to configure the volumes of the seat capillary and the loop capillary Changes must be acknowledged with F6 Done Configure Thermostat F4 Therm in the main Configuration sc...

Page 264: ...e procedures to Change parts on the WPS or additional test Signals to monitor various parameters during the WPS operation Use the F1 key Align Trans to perform an automatic alignment of the WPS transp...

Page 265: ...MWS Reference Manual 265 The alignment process may take several minutes Changes must be acknowledged with F6 Done Use the F2 key Change to access maintenance positions for changing the Needle or samp...

Page 266: ...anual 8 Control Module Screens of the Well plate Samplers Press F3 Signals in the Tests screen to access additional service signal for diagnosing and troubleshooting the WPS Here you can monitor vario...

Page 267: ...jection valve cycles or number of needle movements into the needle seat at which you want to receive a warning Changes must be acknowledged with F6 Done If a set limit has been exceeded a message box...

Page 268: ...ntrol Module Screens of the Well plate Samplers System Error Log Use the F2 key System Log or F3 key Error Log to look for errors For troubleshooting reasons these Logbooks can be printed or saved to...

Page 269: ...his Logbook can be printed or saved to a file on the PCMCIA card pressing the m key Firmware Update Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F3 key...

Page 270: ...micro WPS or 1378nnnn DLB for the thermostatted micro WPS and press execute When the update has finished the update press F8 key Transfer to return the module into the normal mode LED on module shoul...

Page 271: ...not saved your methods please do it before continuing Otherwise they will be overwritten during the update process Changing the serial number In case the serial number of the module has to be added u...

Page 272: ...Reference Manual 8 Control Module Screens of the Well plate Samplers Type in the module s Serial as indicated on the front or rear label of the module using the alphanumeric keys Changes must be ackn...

Page 273: ...273 Agilent 1100 Series Well plate Sampler Micro Well plate Sampler Reference Manual Agilent Technologies 9 Specifications Performance Specifications 274...

Page 274: ...100 l in 0 1 l increments Up to 1500 l with multiple draw hardware modification required Precision Typically 0 5 RSD of peak areas from 5 100 l Typically 1 RSD from 1 5 l Sample viscosity range 0 2 5...

Page 275: ...capillary 0 01 40 l in 0 01 l increments with the extended loop capillary Precision Typically 0 5 RSD of peak areas from 5 40 l Typically 1 RSD from 1 5 l Typically 3 RSD from 0 2 1 l Sample viscosity...

Page 276: ...276 1100 Series WS MWS Reference Manual 9 Specifications...

Page 277: ...277 Agilent 1100 Series Well plate Sampler Micro Well plate Sampler Reference Manual Agilent Technologies A Safety Information General Safety Information 278...

Page 278: ...ument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards WARNING If you are using flammable solvents remove the well plates...

Page 279: ...ause physical injury or damage to the equipment Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A caution alerts you to situations that could c...

Page 280: ...teri af samme fabrikat og type Lever det brugte batteri tilbage til leverandoren WARNING Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten Brukt ba...

Page 281: ...Settings screens 247 Signal Plot screens 255 Status screens 255 System screen 259 Thermostat screen 254 Timetable screens 252 D damaged packaging 17 delay volume reduction 53 delayed injection 53 deli...

Page 282: ...eedle sample transport assembly 207 numbering of vials 32 O operating modes 209 optimizing performance automated needle wash 51 delay volume 51 delayed injection 53 delay volume adjustment 51 early ma...

Page 283: ...lectivity 53 sensors 217 shipping 41 simple repairs 109 site requirements 14 specifications 16 274 stack configuration 21 23 rear view 21 23 stator 205 status indicator 56 status indicators 58 step co...

Page 284: ...284 1100 Series WS MWS Reference Manual Index...

Page 285: ......

Page 286: ...00 Series Well plate Sampler Micro Well plate Sampler and micro well plate sampler The manual describes the following installing the sampler optimizing performance troubleshooting and test functions r...

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