Analytical Notes
Agilent Electrothermal Temperature Controller ETC 60 User’s Guide
39
Optimal Temperature Determination
To determine the optimum temperature for your analysis:
1.
Set up the instrument and install the ETC 60 as described in the ‘Getting
Started’ section.
2.
Set the temperature to 300 °C (100 °C for mercury) and wait for the ETC 60 to
reach the temperature.
3.
Go to the Instrument parameters page, and set the Measurement time to read
30 s, four replicates, and Delay time to 0 s.
4.
Set the signal graphics to display 0–250 sec and an Absorbance range of
−0.20
–1.00 A.
5.
Pump a solution of the analyte (10–20 ppb) through the system.
6.
Press Read and monitor the signal graphics. When a stable signal has been
achieved, increase the temperature by 50 °C and then monitor the signal
graphics.
NOTE
Do not exceed 300 °C when optimizing for mercury.
7.
Repeat Step 6 until the signal strength does not increase with increase in
temperature.
The lowest temperature at which the maximum stable signal is obtained is the
optimum temperature setting for your analysis.
