3
Agilent DNA 1000 Kit Quick Start Guide
Setting up the Chip Priming Station
1
Replace the syringe:
a
Unscrew the old syringe from the lid of the chip priming station.
b
Release the old syringe from the clip. Discard the old syringe.
c
Remove the plastic cap of the new syringe and insert it into the clip.
d
Slide it into the hole of the luer lock adapter and screw it tightly to the chip
priming station.
2
Adjust the base plate:
a
Open the chip priming station by pulling the latch.
b
Using a screwdriver, open the screw at the underside of the base plate.
c
Lift the base plate and insert it again in position C. Retighten the screw.
3
Adjust the syringe clip:
a
Release the lever of the clip and slide it down to the lowest position.
Essential Measurement Practices
•
Handle and store all reagents according to the instructions on the label of the
individual box.
•
Avoid sources of dust or other contaminants. Foreign matter in reagents and
samples or in the wells of the chip will interfere with assay results.
•
Keep all reagents and reagent mixes refrigerated at 4 °C when not in use.
•
Allow all reagents and samples to equilibrate to room temperature for 30 min before use.
•
Protect dye and dye mixtures from light. Remove light covers only when pipetting. The dye decomposes
when exposed to light and this reduces the signal intensity.
•
Always insert the pipette tip to the bottom of the well when dispensing the liquid. Placing the pipette at
the edge of the well may lead to poor results.
•
Use a new syringe and electrode cleaners with each new kit.
•
Use loaded chips within 5 min after preparation. Reagents might evaporate, leading
to poor results.
•
Do not touch the Agilent 2100 Bioanalyzer during analysis and never place it on a
vibrating surface.
Agilent DNA Assay Protocol
WA R N I N G
Handling DMSO
Kit components contain DMSO. Because the dye binds to nucleic acids, it should be treated as a
potential mutagen and used with appropriate care.
➔
Wear hand and eye protection and follow good laboratory practices when preparing and handling
reagents and samples.
➔
Handle solutions with particular caution as DMSO is known to facilitate the entry of organic
molecules into tissues.
