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Agilent Technologies Agilent 1260 Infinity, System User'S Manual

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Agilent Technologies Agilent 1260 Infinity System User'S Manual Download Page 140

© 

Agilent Technologies 2006-2014, 2015

Printed in Germany 
07/2015

*G1312-90303*

*G1312-90303*

G1312-90303
Rev.B

www.agilent.com

Agilent Technologies

In This Book

This manual contains information about the 
Agilent 1260 Infinity Binary LC.

The manual describes the following:

•

Product description,

•

introduction,

•

specifications,

•

system optimization,

•

system setup and installation,

•

quick start guide.

Summary of Contents for Agilent 1260 Infinity

Page 1: ...Agilent Technologies Agilent 1260 Infinity Binary LC System User Guide...

Page 2: ...ed only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and li...

Page 3: ...s 3 Optimization of the Agilent 1260 Infinity Binary LC This chapter considers how to apply the theory and use the features of the LC system to develop optimized separations 4 System Setup and Install...

Page 4: ...3 Optimization of the Agilent 1260 Infinity Binary LC 39 How to Configure the Optimum Delay Volume 40 How to Achieve Higher Injection Volumes 47 How to Achieve Shorter Cycle Times 48 How to Achieve Lo...

Page 5: ...1260 Infinity Binary LC System User Guide 5 Contents 6 Appendix 101 Safety Information 102 Solvent Information 105 Agilent Technologies on Internet 110 Setting Up a Method using Edit Entire Method 111...

Page 6: ...6 1260 Infinity Binary LC System User Guide Contents...

Page 7: ...mponents 10 1260 Infinity Binary Pump G1312B 11 1260 Infinity High Performance Degasser G4225A 14 1260 Infinity High Performance Autosampler G1367E 15 1290 Infinity Thermostatted Column Compartment G1...

Page 8: ...Binary LC are The configurable delay volume down to 120 L in the 1260 Infinity Binary Pump combined with a flow range from 0 05 up to 5 mL min at pressures up to 600 bar provides universal applicabil...

Page 9: ...rsion for narrow bore columns Baseline robustness and fast spectral acquisition at data rates up to 80 Hz through the new optical design of the 1260 Infinity Diode Array Detector Different UV detector...

Page 10: ...the needs of your individual application requirements A few configurations are described in more detail in this manual see System Setup and Installation on page 67 The modules that are described in th...

Page 11: ...adient from one of two solvents per channel Active seal wash optional is available for use with concentrated buffer solutions Principle of operation The binary pump is based on a two channel dual pist...

Page 12: ...System Components Figure 1 The Hydraulic Path of the Binary Pump with Damper and Mixer Ejbe ZVY 6 Ejbe ZVY 7 E hidch E hidch HZVah HZVah gdb WdiiaZ 6 hdakZci caZi kVakZ B m c 8 VbWZg DjiaZi kVakZ Djia...

Page 13: ...mode For instructions on how to change between the two configurations see the G1312B Binary Pump User Manual Figure 2 The Hydraulic Path of the Binary Pump with Bypassed Damper and Mixer NOTE Bypassi...

Page 14: ...pressure is measured by a pressure sensor The vacuum degasser maintains the low pressure by a controlled leak in the air inlet filter and a regulation of the vacuum pump using the pressure sensor The...

Page 15: ...mpling sequence is not completed successfully an error message is generated Solvent is bypassed from the autosampler by the injection valve during the sampling sequence The needle moves to the desired...

Page 16: ...ject and run step The six port valve is switched to the main pass position and directs the flow back through the sample loop which now contains a certain amount of sample The solvent flow transports t...

Page 17: ...ispersion heat exchanger and each valve kit contains additional low dispersion heat exchangers for each column The low dispersion heat exchangers up to 4 can be mounted flexibly inside the TCC For con...

Page 18: ...umns available for different applications Thus the column to be used becomes a simple method parameter This requires two 8 position 9 port valve heads one each in two of the TCCs Clustered TCC are rep...

Page 19: ...caused by refractive index or thermal effects and results in more reliable integration of peak areas Figure 5 The light path through the DAD The module also incorporates electronic temperature contro...

Page 20: ...h Signals can be output at 80 Hz 80 data points second for accurate recording of the fastest narrowest chromatographic peaks At the same time the module can also output full range spectra to the data...

Page 21: ...e applications Each valve head comes as a complete kit containing all required capillaries additional low dispersion heat exchangers and other parts Some typical applications for Quick Change Valves a...

Page 22: ...e a system that exactly meets your individual application requirements This individual configuration can be different from the standard configuration which is described in this System User Guide The p...

Page 23: ...435 mm 7 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 220 VA 74 W 253 BTU Maximum Ambient operating temperature 4 55 C 39 131...

Page 24: ...eater pumping degassed H2O at 10 MPa 100 bar Pressure operating range Operating range 0 60 MPa 0 600 bar 0 8700 psi up to 5 mL min Pressure pulsation 2 amplitude typically 1 3 or 0 3 MPa 3 bar whateve...

Page 25: ...Extensive support for troubleshooting and maintenance is provided by the Instant Pilot Agilent Lab Advisor and the Chromatography Data System Safety related features are leak detection safe leak hand...

Page 26: ...26 1260 Infinity Binary LC System User Guide 1 The Agilent 1260 Infinity Binary LC Product Description Specifications...

Page 27: ...chnologies 2 Introduction Theory of Using Smaller Particles in Liquid Chromatography 28 Benefits of small particle size columns 34 Frictional Heating 37 This chapter gives an introduction to the Agile...

Page 28: ...easing the flow rate without losing separation performance 2 Higher Resolution Long columns with sub two micron particles provide higher efficiency and therefore higher resolution which is required fo...

Page 29: ...diffusion or multiple flow paths through the column B relates to molecular diffusion along the column axis longitudinal C relates to mass transfer of the analyte between the mobile and stationary phas...

Page 30: ...analysis time is also reduced by a factor of two to three The increased efficiency is derived to a large extent from the reduction in multiple flow paths as a result of the smaller particles this lead...

Page 31: ...mics of a separation can be considered and the properties of the solute and the stationary and mobile phases percentage of organic solvent ion strength and pH can be manipulated to achieve the shortes...

Page 32: ...N selectivity retention factor k According to the resolution equation Figure 9 on page 32 the selectivity has the biggest impact on resolution Figure 10 on page 32 This means that the selection of ap...

Page 33: ...contrast to totally porous silica columns these SPP columns have a solid core 1 7 m in diameter and a porous silica layer 0 5 m thickness surrounding it Speed and resolution of PoroShell columns are...

Page 34: ...lent Technologies web site along with a method translator calculator www chem agilent com The results are presented graphically and in tabular form Shorter run times also deliver faster answers This i...

Page 35: ...between particle size efficiency and analysis time Analysis time can be shortened without sacrificing column efficiency by optimizing particle size and pressure 8dajbc AZc i bb 8dajbc 8dajbc 8dajbc X...

Page 36: ...such as impurity profiling can benefit from higher separation power Even the LC MS analysis of drugs in biological fluids can benefit from the higher peak capacity because of the reduced interference...

Page 37: ...emperature gradient and therefore reduces the efficiency losses but a higher column outlet temperature has to be accepted The raised temperature may have an effect on selectivity At lower back pressur...

Page 38: ...38 1260 Infinity Binary LC System User Guide 2 Introduction Frictional Heating...

Page 39: ...ion for 3 and 4 6 mm i d Columns 45 How to Achieve Higher Injection Volumes 47 How to Achieve Shorter Cycle Times 48 How to Achieve High Throughput 50 How to Achieve Lowest Carry over 51 How to Achiev...

Page 40: ...es and small column volumes and can have a large impact on the transferability of gradient methods It is important therefore for fast gradient separations to have small delay volumes especially with n...

Page 41: ...columns require proportionally smaller extra column volumes to keep peak dispersion at a minimum In a liquid chromatograph the extra column volume will depend on the connection tubing between the aut...

Page 42: ...illaries automatically using a 600 bar 2PS 6PT valve optional Table 4 Delay volumes of the 1260 Infinity Binary LC modules Components Delay Volume L Binary Pump1 1 in low delay volume configuration wi...

Page 43: ...rtant to select the respective Enhanced Solvent Compressibility function in the auxiliary screen of the pump menu To minimize peak dispersion the low dispersion kit Low dispersion kit G1316 68744 must...

Page 44: ...L mixer Low volume mixer 200 L 5067 1565 can be installed to reduce any residual mixing noise This small mixer gives the lowest UV baseline noise even under extreme gradient conditions See Figure 14...

Page 45: ...mm inner diameter columns are about two and five times larger respectively than for the same length 2 1 mm i d columns and the flow rates that are used are also proportionally higher Therefore the sta...

Page 46: ...or vice versa Figure Figure 16 on page 46 shows two overlaid chromatograms of a method for analysis of paracetamol and related impurities The method was transferred from an Agilent 1200 Series LC syst...

Page 47: ...starting mobile phase and any increase will affect the separation particularly for early running peaks low retention factor In some cases it is the cause of peak distortion and the general rule is to...

Page 48: ...ume mixer 200 L 5067 1565 Chromatographic conditions strongly depend on the compounds that are to be analyzed but some rules of thumb can be used to achieve short run times The flow rates should be as...

Page 49: ...of the delay volume The lower the flow rate the greater the negative impact that can be expected from delay volume In Figure 17 on page 49 a 2 1 mm inner diameter column was used at a flow rate of 0 6...

Page 50: ...e before the injection valve is switched to the bypass mode should be increased using the flush out factor to 20 This extends the time during which the autosampler delay volume is flushed with mobile...

Page 51: ...ny application involving compounds liable to stick in the system The following functions of the autosampler can be used to minimize carryover Internal needle wash External needle wash Needle seat back...

Page 52: ...ring aspiration of the sample that the injection valve is switched out of the flow path In this position the pump effluent is led directly to the column Prior to injection the outside surfaces of the...

Page 53: ...s is described by the resolution equation where Rs resolution N plate count measure of column efficiency selectivity between two peaks k2 retention factor of second peak formerly called capacity facto...

Page 54: ...rate more back pressure than acetonitrile mixtures Acetonitrile is often preferred because peak shapes are better and narrower in addition to the lower viscosity but methanol generally yields better s...

Page 55: ...same but happens in half the time Any reduction in extra column volume will reduce dispersion and give better resolution This is already optimized in the 1260 Infinity Binary LC with narrow bore 0 12...

Page 56: ...umn effluent should be cooled down to the temperature of the detector by using the 1 5 L cooler heater in the column compartment This ensures lowest noise level with UV detectors even at 5 mL min and...

Page 57: ...alled in the column compartment For highest UV sensitivity the short mixer is also recommended See Figure 13 on page 43 and Figure 14 on page 44 The injection volume is also of importance especially i...

Page 58: ...ate that is too low results in increased peak width and lower resolution see Figure 19 on page 58 Figure 19 Peak width vs UV data Rate Table 6 on page 59 shows the dependency of peak width resolution...

Page 59: ...that elevated flow rates can also be advantageous for improved separation For 4 6 mm inner diameter columns packed with sub 2 micron particles a flow rate of 2 mL min is recommended as a starting val...

Page 60: ...ty How to Achieve Higher Sensitivity The sensitivity of a separation method is linked to the choice of stationary and mobile phases as good separation with narrow peaks and a stable baseline with mini...

Page 61: ...n of the peaks on the column This also creates peak dispersion and a lower peak height The data rate of the UV detector should be selected according to the actual peak width Higher than necessary data...

Page 62: ...t can be injected on a smaller i d column then the dilution due to column diameter will be less and the sensitivity will increase For example decreasing the column i d from 4 6 mm to 2 1 mm results in...

Page 63: ...owering the limit of detection LOD by a factor of about 3 depending on the application Table 7 Specifications for Max Light Cartridge Flow Cells Cartridge Cells Max Light Cartridge Cell 10 mm V s 1 0...

Page 64: ...ists as 1290 Infinity Ultra Low Dispersion Kit 5067 5189 The cell should be part of the ultra low dispersion solution High Dynamic Range The Max Light Cartridge HDR cell can be used with the G4212A DA...

Page 65: ...hase 50 mM in combination with high acetonitrile concentrations due to possible precipitation 6 An in line filter is recommended to catch particulates and extend column life Use the suitable 1260 Infi...

Page 66: ...ion of the Agilent 1260 Infinity Binary LC How to Prevent Column Blockages Figure 20 Protection for 4 6 and 2 1 mm id columns packed with 1 8 m particles inlet frit with 0 2 m pore size chZgih l i hbV...

Page 67: ...ling Software 68 Installing the Modules 70 Installing the System Modules 70 Integration Into the Network 70 Capillary and Tubing Connections in Flow Path 71 Priming the System 76 This chapter includes...

Page 68: ...Guide 4 System Setup and Installation Installing Software Installing Software Installing the Software Controller and Data System For details of installation procedures for the software refer to the de...

Page 69: ...ounters This allows a problem to be fixed before it impacts results The software includes an extensive suite of user information and documentation a set of calculators and tools to help set up calibra...

Page 70: ...ling the System Modules For details of installation procedures for the modules refer to the individual module manuals These manuals also contain information on specifications maintenance and parts Int...

Page 71: ...ation 4 Installing the Modules Capillary and Tubing Connections in Flow Path Depending on the system configuration capillaries of different lengths and diameters are used These are described below See...

Page 72: ...ion Figure 21 on page 72 shows capillary and tubing connections in the flow path for the standard delay volume configuration of the 1260 Infinity Binary LC Figure 21 Capillary and tubing connections i...

Page 73: ...0 17 mm x 700 mm S S Pump to cooled Autosampler 2 G1367 87304 Capillary ST 0 17 mm x 250 mm S S Autosampler to TCC 2 01090 87306 Capillary ST 0 17 mm x 380 nm S S Cooled Autosampler to TCC 3 G1316 87...

Page 74: ...on Figure 22 on page 74 shows capillary and tubing connections in the flow path for medium and low delay volume configurations of the 1260 Infinity Binary LC Figure 22 Capillary and tubing connections...

Page 75: ...ST 0 12 mm x 180 mm S S Autosampler to TCC 2 01090 87610 Capillary ST 0 12 mmx 280 mm S S cooled Autosampler to TCC or to item 3 if installed 3 G1316 80002 Heater long up 0 12 mm i d 1 6 L internal vo...

Page 76: ...lvents and reagents can carry health risks Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sh...

Page 77: ...channel A1 is advancing towards the pump If it isn t disconnect the solvent tubing from the solvent selection valve attach a syringe with a syringe adapter and pull the liquid through the degasser Rea...

Page 78: ...d flow rate for your application and close the purge valve 5 Pump for approximately 10 minutes before starting your application When When the pumping system has been turned off for a certain time for...

Page 79: ...rate for your application and equilibrate the system for approx 10 minutes prior to starting a run When When the solvent of a channel is to be replaced by another solvent that is not compatible solven...

Page 80: ...solvent to flush air out of the system Miscible with almost all solvents After an installation Ethanol or methanol Alternative to isopropanol second choice if no isopropanol is available To clean the...

Page 81: ...ine Plot 85 Purging the Pump 87 Data Acquisition in Method and Run Control View 88 Method Parameters for System Checkout with Isocratic Checkout Sample 88 Setting Up the Method 92 Running the Method f...

Page 82: ...ysis using the columns and checkout sample that are provided with the Agilent 1260 Infinity Binary LC The example refers to menus and commands in OpenLAB CDS ChemStation Edition but identical function...

Page 83: ...rt All Programs Agilent Technologies OpenLAB OpenLAB Control Panel Select the relevant instrument in the navigation pane under Instruments and click Launch online The ChemStation software opens in the...

Page 84: ...Method and Run Control view of the ChemStation 2 On the menu bar select Method New Method and select DEF_LC M from the context menu Alternatively you can use the Load Method icon under the menu bar or...

Page 85: ...eparing the System Configuring the Online Plot 1 If the Online Plot window is not visible Click View Online Signals Signal Window 1 to display the window 2 To configure the desired signal s in the Onl...

Page 86: ...s is useful for checking the baseline and looking at previous injections The X and Y axis scales can be adjusted directly with the up down buttons on each axis The Adjust button in the Online Plot win...

Page 87: ...few hours or more air may have diffused into the solvent lines and purging is recommended The solvent reservoirs are refilled and the pump requires purging to fill the system with fresh solvent If dif...

Page 88: ...tic test mixture Agilent isocratic checkout sample 01080 68704 will be performed on the column ordered and delivered with your system Agilent isocratic checkout sample 01080 68704 contains the followi...

Page 89: ...olvent B Acetonitrile Flow rate 4 0 mL min Solvent Composition 40 A 60 B Stop Time 1 min 1 min post time Autosampler Injection 1 L Column Compartment Column Column Eclipse Plus C18 4 6 x 100 mm 3 5 m...

Page 90: ...parameters for first separation run low delay volume configuration Module Parameter Setting Pump Solvent A Water Solvent B Acetonitrile Flow rate 0 75 mL min Solvent Composition 40 A 60 B Stop Time 1...

Page 91: ...e supplied syringe 3 Open the purge valve of the pump and purge each channel at 5 mL min This should be sufficient to remove air in the system 4 Install the supplied column into the column compartment...

Page 92: ...d in the context menu a In the Method page for the 1260 Infinity Binary Pump enter the following parameters Flow rate 4 0 ml min Solvent A Select Water from the compressibility drop down list Solvent...

Page 93: ...or the 1260 Infinity DAD enter the following parameters Use Signal Turn all signals except Signal A off by clearing the check boxes Signal A 254 nm bw 4 nm ref 550 nm bw 100 nm Peak width 0 02 b In th...

Page 94: ...menu bar to load them 3 Place the sample vial in position 1 this is the front position of the 10 x 2 ml vial positions on the right hand side of the sample tray 4 Select Run Control Sample Info and e...

Page 95: ...Start Guide 5 Data Acquisition in Method and Run Control View 6 The injection is made and the chromatogram appears in the Online Plot The data acquisition will stop when the Stop Time is reached The...

Page 96: ...g the data to give quantitative and qualitative results This section looks briefly at integration and reports in data analysis so that the separations generated earlier in this chapter can be integrat...

Page 97: ...lick Data Analysis in the bottom left of the screen 3 In the Navigation Panel find the data directory containing the data files All the single injection data are represented as a subset called Single...

Page 98: ...icon to open the table as shown 3 Set Baseline Correction to Advanced for gradient runs 4 Set Slope Sensitivity to 5 Higher numbers will integrate steeper peaks and ignore less steep peaks 5 Set the...

Page 99: ...1260 Infinity Binary LC System User Guide 99 Quick Start Guide 5 Data Analysis Specify the Report 1 On the menu bar click Report Specify Report to display the window shown in the figure below...

Page 100: ...ally a directory The report file can be viewed directly in ChemStation or it can be found in the directory using the normal Windows File Explorer 4 Save the method once again to ensure the report sett...

Page 101: ...rmation 105 Material Information 105 Agilent Technologies on Internet 110 Setting Up a Method using Edit Entire Method 111 Method Information 113 Instrument Acquisition 114 Data Analysis 129 Run Time...

Page 102: ...andards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Safety Standards This is a Safet...

Page 103: ...ailable at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this...

Page 104: ...e damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not...

Page 105: ...itions For any special conditions please consult the material information section or contact Agilent Disclaimer Subsequent data were collected from external resources and are meant as a reference Agil...

Page 106: ...ont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e...

Page 107: ...ke nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against...

Page 108: ...y strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with s...

Page 109: ...used in all Agilent degassers except G1322A are not compatible with fluorinated solvents like Freon Fluorinert or Vertrel They have limited life time in the presence of Hexafluoroisopropanol HFIP To e...

Page 110: ...ry LC System User Guide 6 Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at...

Page 111: ...r through the menu bar with its drop down menus A new method can be created by loading and editing an existing method or it can be created by loading and editing the blank template method DEF_LC M To...

Page 112: ...nstrument curves Data Analysis comprises signal details integration parameters and reporting parameters Run Time Checklist comprises the parts of the method that will be executed NOTE During Edit Enti...

Page 113: ...o be directly accessed through the menu Method Method Information or by right clicking on the graphical user interface This box allows information about the method to be entered This information will...

Page 114: ...Binary Pump BinPump Thermostatted Column Compartment TCC Diode array Detector DAD Instrument Curves To move between the tabs click on the tab name at the top of the screen When parameter changes have...

Page 115: ...er This is the recommended option and is configured in the next entry Needle Wash if selected above Mode determines how the outside of the needle will be rinsed either actively in the Flush Port or by...

Page 116: ...e the sample from near the bottom of the vial With the HiP ALS for example a value of 0 would place the tip of the needle 1 mm above the bottom of a wellplate Equilibration time is a delay time betwee...

Page 117: ...he column sooner Enable overlapped injection also switches the injection valve from mainpass to bypass after the injection has taken place either after the sample has flushed out of the injector or at...

Page 118: ...s specialized injection procedures to be constructed which involve the manipulation of aliquots from multiple vials as for instance in pre column derivatization Reagent chemicals are automatically mix...

Page 119: ...n Binary Pump tab Flow sets the flow rate up to 5 mL min For the example separation 4 mL minis used If the back pressure briefly reaches the maximum pressure setting the flow will be reduced for a few...

Page 120: ...e composition of A and B in the mobile phase or if required to the flow rate and maximum pressure allowed The timetable makes linear changes to the parameters between the given setpoints The settings...

Page 121: ...ters the Stop Time in the pump is considered to be the master and the other modules will usually be set to follow this value Post Time defines a countdown period after the end of a run during which th...

Page 122: ...ary when the column is longer than 15 cm and needs to be supported by both sections The two sides can be operated separately when it is required to have two columns operating at different temperatures...

Page 123: ...to 0 15 C down to 10 C below ambient although it should be noted that there are very few applications operating below 12 15 C Avoid using the TCC at such low temperature that condensation of water fro...

Page 124: ...Array Detector tab Signals Up to eight separate signals chromatograms can be recorded To mark a signal for collection check the Use Signal box for that signal define the wavelength and bandwidth and...

Page 125: ...d which takes B back down to the initial value over two minutes to gently start the re equilibration of the column No useful data is expected during this downwards ramp so the detector stop time is se...

Page 126: ...sary to have signal A set as a broadband detector to ensure that peak spectra are available for all the different wavelength signals Store controls the spectral collection mode with the following opti...

Page 127: ...flat lining at the bottom of the range However it should not be changed without good reason as making it more negative will increase baseline noise and reduce the range available for measuring positi...

Page 128: ...llows monitored data streams other than detector signals to be stored with the data by checking the relevant box These are primarily used for diagnostic purposes They are Pump Pressure Flow A B Compos...

Page 129: ...wer part of the screen You can select any or all of the acquired detector signals for processing If no signals are selected the table is empty in this situation ChemStation will default to processing...

Page 130: ...130 1260 Infinity Binary LC System User Guide 6 Appendix Setting Up a Method using Edit Entire Method Figure 31 Signal details...

Page 131: ...n the context menu In the Data Analysis view it can be accessed through the menu Integration Integration Events or the Edit Integration Events task icon Figure 32 Edit Integration Events screen Integr...

Page 132: ...f the baseline needed to mark the start and end of a peak Peak Width the width at half height of the narrowest peak of interest should be entered This helps the integrator to distinguish between noise...

Page 133: ...rt in the context menu In the Data Analysis view it can be accessed through the menu Report Specify Report or the Specify Report task icon Figure 33 Specify Report Screen To setup a simple area report...

Page 134: ...t Size Time axis 100 of page Response axis 40 of page Destination Printer Checked Screen Unchecked File Checked File Setting PDF Checked Unique PDF file name Checked On the Quantitation settings tab C...

Page 135: ...er Guide 135 Appendix 6 Setting Up a Method using Edit Entire Method Instrument Curves Figure 34 Instrument Curves screen The Instrument Curves checkboxes allow these recorded parameters to be overlai...

Page 136: ...opportunity to link macro commands or programs into the work flow at various points In most cases the Data Acquisition and Standard Data Analysis check boxes are checked If no data analysis is requir...

Page 137: ...sual configuration as the software always saves the method in the data file all versions since B 02 01 Only if the ChemStation has been configured such that Unique Sequence Folder Creation is turned o...

Page 138: ...volume 45 configuring online plot 85 control and data evaluation 25 control assembly 14 cycle time instrument configuration 48 D data analysis 112 data analysis 96 data evaluation and control 25 DEF_L...

Page 139: ...range 24 physical specifications 23 power consumption 23 pressure sensor 14 pressure operating range 24 pulsation 24 priming with a pump 78 principle of operation 14 purging 87 Q quick start guide in...

Page 140: ...12 90303 Rev B www agilent com Agilent Technologies In This Book This manual contains information about the Agilent 1260 Infinity Binary LC The manual describes the following Product description intro...

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