Agena BIOSCIENCE MassARRAY Nanodispenser RS1000, User Manual, Page: 43 / 162

Chapter 2 Dispensing to SpectroCHIP Arrays
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USG-CUS-059 Rev05 MassARRAY® Nanodispenser RS1000 v2.1 User Guide
For Research Use Only. Not for use in diagnostic procedures.
NOTE NOTE
Since the resin levels may have been adjusted in the preceding procedure ( Checking the resin
and liquid levels, page 39 ), it is assumed that the resin in the microtiter plate wells comes up no
higher than 4.5-5.0 mm (6 mg resin plates) from the bottom of wells.
NOTE NOTE
The instrument assumes measurements are from the top of the well down. However, it is physically
easier to measure from the bottom of wells. You will convert your measurement to the instrument’s
top-down scheme in the next step.
4. Add 0.5 mm to the resin height obtained in step 3 and subtract that sum from the well
depth. This is the aspirate offset.
NOTE NOTE
Figure 2.31 and Figure 2.32 show example aspirate offset calculations for a specific type of plate.
For a different brand of plate, substitute its well height (usually available in technical specifications
on the manufacturer’s website).
Figure 2.30 Measuring the Height of Clean Resin in a Microtiter Plate Well
Figure 2.31 Aspirate Offset Example Calculation
This example calculates the aspirate offset for a 384-well microtiter plate (Thermo
Scientific AB-1384).
Figure 2.32 Aspirate Offset Example Calculation
This example calculates the aspirate offset for a 96-well microtiter plate (Thermo
Scientific AB-0800).
Measure this distance
Resin
9.25 mm
4.25 mm resin height
Aspirate offset = 9.25 mm
-
(resin 0.5 mm) = 4.5 mm
15.00 mm
4.0 mm resin height
Aspirate offset = 15.00 mm
-
(resin 0.5 mm) = 10.5 mm