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AFi AFI-C300R User Manual Download Page 6

 Originale Instructions-  Rev.1.3 

User Maunal– Loreena  

4/ 68

 

 

Table of Contents 

 

1. 

G

ENERAL 

I

NFORMATION

 ------------------------------------------------------------------------------------------------------ 6 

1.1.

 

D

ESCRIPTION

 ....................................................................................................................................................... 6

 

1.2.

 

I

NTENDED USE

 ..................................................................................................................................................... 6

 

1.3.

 

P

ROHIBITIONS

: .................................................................................................................................................... 7

 

1.4.

 

M

ODELS

,

 

S

PECIFICATIONS AND 

A

CCESSORIES

 ............................................................................................................. 8

 

1.4.1.

 

M

ODELS 

&

 

S

PECIFICATIONS

 ................................................................................................................................... 8

 

1.4.2.

 

A

CCESSORIES

 ....................................................................................................................................................... 9

 

1.5.

 

S

AFETY 

I

NSTRUCTIONS

 ......................................................................................................................................... 12

 

1.6.

 

I

NFORMATION ON THE REFRIGERANT FLUID USED

 ...................................................................................................... 12

 

2. 

I

NSTALLATION 

I

NSTRUCTIONS

 ---------------------------------------------------------------------------------------------- 13 

2.1.

 

S

HIPMENT

 ........................................................................................................................................................ 13

 

2.1.1.

 

D

AMAGED 

S

HIPMENTS

 ........................................................................................................................................ 13

 

2.1.2.

 

H

ANDLING AND TRANSPORT

 ................................................................................................................................. 13

 

2.2.

 

S

ITE PREPARATION

 .............................................................................................................................................. 13

 

2.3.

 

E

LECTRICAL ENVIRONMENT

 .................................................................................................................................. 14

 

2.4.

 

U

NPACKING

 ...................................................................................................................................................... 14

 

2.5.

 

P

ACKING LIST

 ..................................................................................................................................................... 14

 

2.6.

 

D

ESCRIPTION

 ..................................................................................................................................................... 15

 

3. 

O

PERATING THE 

LOREENA

 CENTRIFUGE 

-

 

AFI-C300 ------------------------------------------------------------------- 16 

3.1.

 

F

IRST 

S

TART 

/

 

P

OWERING

 .................................................................................................................................... 16

 

3.2.

 

L

EVELLING 

(F

LOOR MODEL

) .................................................................................................................................. 17

 

3.3.

 

R

OTOR ASSEMBLY

 ............................................................................................................................................... 17

 

3.4.

 

P

OSITION THE BUCKETS

 ....................................................................................................................................... 17

 

3.5.

 

F

IRST POWERING

 ................................................................................................................................................ 17

 

3.6.

 

U

SING THE INTERFACE

 ......................................................................................................................................... 18

 

3.7.

 

O

PENING THE LID

 ............................................................................................................................................... 18

 

3.8.

 

R

OTOR LOADING AND BALANCING

 ......................................................................................................................... 18

 

3.9.

 

Q

UICK START

-

UP

 ................................................................................................................................................ 19

 

4. 

O

PERATING

 ------------------------------------------------------------------------------------------------------------------ 20 

4.1.

 

S

ETTINGS

 .......................................................................................................................................................... 20

 

4.2.

 

C

REATE 

/

 EDIT A PROGRAM NAME

 ......................................................................................................................... 20

 

4.3.

 

S

ET PROGRAM SETTINGS

 ...................................................................................................................................... 21

 

4.4.

 

S

ET SPEED IN 

RPM

 OR 

RCF ................................................................................................................................. 22

 

4.5.

 

P

ROTECT THE SETTINGS OF A PROGRAM

 .................................................................................................................. 23

 

4.6.

 

C

ENTRIFUGE

:

 

U

SE A PROGRAM

 ............................................................................................................................. 23

 

4.7.

 

I

NTERRUPT CENTRIFUGATION

 ................................................................................................................................ 24

 

4.8.

 

O

PEN AFTER AN ELECTRIC POWER FAILURE 

/

 

E

MERGENCY 

S

TOP

 ................................................................................... 24

 

4.9.

 

P

RECOOLING

 ..................................................................................................................................................... 25

 

4.10.

 

P

REWARMING

 ................................................................................................................................................... 25

 

4.11.

 

R

OUTINE 

M

ENU

................................................................................................................................................. 26

 

4.12.

 

O

THER MENUS

 ................................................................................................................................................... 26

 

4.12.1.

 

U

SER 

P

REFERENCES

 ............................................................................................................................................ 28

 

4.12.2.

 

T

YPE OF 

S

PEED DISPLAYED 

(RPM

 OR 

RCF) ............................................................................................................. 29

 

4.12.3.

 

F

REQUENCY OF LUBRICATION

 ................................................................................................................................ 30

 

4.12.4.

 

A

CCESS TO LOGBOOK

 .......................................................................................................................................... 30

 

4.12.5.

 

A

CCESS TO ERROR LOGBOOK

 ................................................................................................................................. 30

 

4.12.6.

 

T

YPE OF 

T

EMPERATURE DISPLAYED

 ........................................................................................................................ 31

 

4.12.7.

 

T

IMER MODE

 ..................................................................................................................................................... 31

 

4.12.8.

 

C

OUNTING MODE

 ............................................................................................................................................... 32

 

4.12.9.

 

K

EYBOARD TYPE

 ................................................................................................................................................. 32

 

4.12.10.

 

C

HANGE BACKGROUND COLOR

 .............................................................................................................................. 33

 

4.12.11.

 

E

ND OF 

C

YCLE 

A

LARM

 ......................................................................................................................................... 33

 

4.12.12.

 

E

RROR 

M

ESSAGE 

A

LARM

 ..................................................................................................................................... 33

 

4.12.13.

 

A

UTOMATIC LID OPENING

 .................................................................................................................................... 33

 

4.12.14.

 

S

LEEP MODE

 ...................................................................................................................................................... 34

 

4.12.15.

 

P

OST 

C

OOL

 ....................................................................................................................................................... 34

 

4.12.16.

 

P

RE 

C

OOL 

F

UNCTION

 .......................................................................................................................................... 35

 

4.12.17.

 

T

EMPERATURE 

D

ISPLAY 

M

ODE

 ............................................................................................................................. 35

 

Summary of Contents for AFI-C300R

Page 1: ...User Manual Revision 1 3 English Version...

Page 2: ...rifuge refuses to accept any responsibility in the event of errors or omissions The same applies to any damage resulting from the use of information contained in this manual AFI Centrifuge 3 Rue Nicol...

Page 3: ...Loreena 1 68 LOREENA CENTRIFUGE Models AFI C300V AFI C300R AFI C300RF AFI C300V E AFI C300R E AFI C300RF E Operation Manual 115 VAC 60 Hz 230 VAC 50 Hz Manufactured by AFI Centrifuge Rue N Copernic ZI...

Page 4: ...Date Revision Modifications Paragraph Author August 2018 1 3 End of cycle acquitment Precool Warmup new shortcuts routine program End of cycle Flashing signal information menu modifications Add new ro...

Page 5: ...nitial installation and the serial and model number of the equipment This warranty shall not apply to any AFI Centrifuge product or part thereof which has been subject to misuse abuse accident shippin...

Page 6: ...NG THE LID 18 3 8 ROTOR LOADING AND BALANCING 18 3 9 QUICK START UP 19 4 OPERATING 20 4 1 SETTINGS 20 4 2 CREATE EDIT A PROGRAM NAME 20 4 3 SET PROGRAM SETTINGS 21 4 4 SET SPEED IN RPM OR RCF 22 4 5 P...

Page 7: ...NUFACTURER 45 6 ELECTRICAL ENVIRONMENTAL REQUIREMENTS 46 6 1 ELECTRICAL SUPPLY VOLTAGE FLUCTUATIONS NOT TO EXCEED 10 46 6 2 OPERATIONAL PERFORMANCE FOR INDOOR USE ONLY 46 6 3 LIGHT EXPOSURE 46 6 4 INS...

Page 8: ...rigerant used is CFC free to meet regulations of refrigerant use Benchtop ventilated model Benchtop Refrigerating model Floor Refrigerating Model LOREENA Centrifuges 1 2 Intended use The centrifuge is...

Page 9: ...use Rotating rotors and accessories that are not maintained according to the instructions in this manual or showing signs of wear and or corrosion Rotating rotors and accessories not supplied by AFI C...

Page 10: ...ed rpm 15 300 rpm with AFI RA30 2 rotor 16 000 rpm with AFI RA30 2 rotor Maximum Relative Centrifugal Force g 25 648 xg with AFI RA30 2 rotor 28 048 xg with AFI RA30 2 rotor Dissipated Heat Btuh 1 Btu...

Page 11: ...of 4 AFI 4T10 14ml Urine Tube 156 x 14 ml Set of 4 AFI 4T10 10ml Blood Collection Tube 156 x 10 ml Set of 4 AFI 4T5 5 7ml Blood Collection Tube 200 x 5 ml Set of 4 AFI 4T2 1 5 2ml MicroTube 200 x 2 m...

Page 12: ...Set of 6 AFI 100R50 OAK 6 x 50ml 50ml AOR Advanced Oak Ridge 6 x 50ml Set of 6 AFI 100R30 OAK 6 x 30ml 30ml AOR Advanced Oak Ridge 6 x 30ml Set of 6 AFI 100R15 C 6 x 15ml 15ml Conical Tube 6 x 15ml S...

Page 13: ...R10 10ml Blood Collection Tube 30 x 10 ml AFI 15R5 5 7ml Blood Collection Tube 30 x 5 ml Rotor NU RA16 5 Angular Rotor 16 x 5 ml Rotor NU RA48 2 Angular Rotor 48 x 2 ml Adapters AFI 48R05 0 5ml MicroT...

Page 14: ...ventilated area on a horizontal rigid support to absorb the vibrations generated by the centrifuge During operation the centrifuge must not be moved or be subject to impact Do not attempt to open the...

Page 15: ...accept a claim for loss or damage If the carrier will not perform the inspection an affidavit must be prepared stating that he was contacted on a certain date and that he failed to comply with the req...

Page 16: ...nd should remain readily accessible The electrical outlet should be on its own branch circuit protected with a circuit breaker at the distribution panel near the centrifuge 2 4 Unpacking Remove the ca...

Page 17: ...2 6 Description Fig 1 Refrigerating centrifuge 1 Lid 2 Window 3 Control panel 4 Electronic warhead 5 Main switch 6 Cooling unit Refrigerating model only 7 Electrical outlet Fig 2 Floor centrifuge 8 W...

Page 18: ...Rev 1 3 User Maunal Loreena 16 68 3 Operating the LOREENA centrifuge AFI C300 3 1 First Start Powering The first start must fulfill the conditions of the IEC 61010 2 020 safety standard HOW TO GREASE...

Page 19: ...aft by depositing a thin film of the food grade grease supplied Place the rotor above the drive shaft Slide the rotor on the drive shaft A click indicates that the rotor is locked Clean and lubricate...

Page 20: ...ped with its bucket Do not use a rotor without its complete set of four buckets Maximum loads according to the type of rotor are specified in the annex The centrifuge tolerates an imbalance of 15 gram...

Page 21: ...Originale Instructions Rev 1 3 User Maunal Loreena 19 68 3 9 Quick start up Before using the centrifuge make sure it has been properly installed...

Page 22: ...n Control Select the program to create edit Note 99 programs are available from 01 to 99 ou The program can be modified when the protection icon is an open lock Edit the title screen by pressing on th...

Page 23: ...celeration Centrifuge speed in RPM or RCF Cycle time Setpoint temperature only on the refrigerating model Change the value e g speed Confirm Confirm settings Note To validate without saving the change...

Page 24: ...adius will be displayed below the picture of the rotor The default rotor setting is Swing out rotor RX 750 Radius 184 mm Modify the rotor or radius pressing on the icon Rotor Note This icon changes wh...

Page 25: ...ont of the program Note To remove the protection enter the program through an Admin user profile and press on the closed lock for 3 seconds It will then re open You are now able to modify the program...

Page 26: ...stops Acquit pressing onto the screen or start stop button Or Press lid button to open 4 8 Open after an electric power failure Emergency Stop This operation must be performed by a person trained in c...

Page 27: ...gation allows to cool the centrifuge down to 4 degrees Celsius so it is ready for temperature sensitive samples Visual Instruction Control cooling Select the pre cooling program Rotor Standard Precool...

Page 28: ...oth set on 9 and cannot be modified Start the cycle by pressing The temperature inside the bowl will show on the display 4 11 Routine Menu The routine program allows you to modify the cycle parameters...

Page 29: ...Originale Instructions Rev 1 3 User Maunal Loreena 27 68 Access to other menus by pressing Select the menu User Preferences Service menu Information Go back to the centrifugation screen...

Page 30: ...he following icons Type of speed displayed RPM or RCF G force Frequency of Lubrication Logbook Error logbook Type of temperature displayed Celsius or Fahrenheit Timer settings Counting mode Keyboard t...

Page 31: ...2 Type of Speed displayed RPM or RCF Visual Instruction Control Select the unit you want displayed by default RPM Rotation Per Minute RCF Relative Centrifugal Force or G Force Confirm Do not turn the...

Page 32: ...g out rotor SWING OUT CYCLE COUNT 4 000RPM Quantity of cycles ran with a swing out rotor faster than 4 000RPM RUNNING HOURS Amount of hours the centrifuge has been used COLD MODULE RUNNING HOURS Amoun...

Page 33: ...rol Select you unit you want displayed by default C Degree Celsius F Degree Fahrenheit Confirm 4 12 7 Timer mode Visual Instruction Control Select the type of timer ON The timer only starts when the c...

Page 34: ...opwatch All programs will work according to the chosen mode UP The stopwatch counts from 0 DOWN The stopwatch counts down from the selected cycle time This is the default setting 4 12 9 Keyboard type...

Page 35: ...12 Error Message Alarm This function activates a beep when an error message appears ON Upon error the signal is triggered Pressing the key stops the signal OFF No signal upon error This is the defaul...

Page 36: ...g button This is the default setting Validate 4 12 15 Post Cool This function maintains the temperature in the bowl between runs ON After closing the lid the refrigeration the unit will maintain the t...

Page 37: ...Temperature Display Mode Allow for display of air or rotor temperature AIR The temperature displayed is the temperature of the air in the bowl This is the default ROTOR The temperature displayed is th...

Page 38: ...roller Board Id ID of the microcontroller electronic board Power Board Id ID of the electronic power board Accelerometer Board Id ID of the electronic board of the accelerometer Return 4 15 Energy sav...

Page 39: ...ance products Users should not use cleaning or decontamination methods different from those recommended by the manufacturer as they may cause damage to the unit The following products and materials ar...

Page 40: ...e User Preferences menu To prolong the life of accessories you can then place a thin film of silicone spray or lanolin Rotors buckets inserts can be autoclaved at 122 5 C 252 F 215 kPa for 15 minutes...

Page 41: ...ts are marked on the accessories Visual Instruction Control When there is only 2 000 cycles left before the end of the theoretical life time of the rotor this screen will be displayed It shows the amo...

Page 42: ...components must be replaced 5 5 Storage of accessories Do not store the accessories on a wire rack Oxidation build up may appear and show premature wear of accessories 5 6 Annual maintenance A period...

Page 43: ...s then determined It appears under the button 15 gr e g 480 6 Open the lid and remove the 15 gram weight High threshold 7 Place the 25 gram weight in the same nacelle 8 Proceed in the same way as for...

Page 44: ...chnician 03 BOWL OVERTEMPERATURE The temperature in the bowl is higher than 43C the room temperature is too high Wait until complete stop Turn the AC or Ventilation system on to obtain an ideal ambien...

Page 45: ...good The speed signal was lost during rotation 11 WRONG SPEED SIGNAL The speed sensor is not working properly The board with tachometer accelerometer is working improperly The connection to these part...

Page 46: ...nal Loreena 44 68 Never try to disassemble or fix the unit on your own the risks of getting injured or electrocuted are high The manufacturer warranty would then be void Only a certified technician is...

Page 47: ...0 Gas spring kit ESL 3 years 71123101 Shock Absorber kit RF ESL 5 years 71199012 Grease tube for accessories 20 gr Consumable 71199003 Disinfectant Consumable ESL Estimated Service Life in years befor...

Page 48: ...ply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and resear...

Page 49: ...sed of with household waste For easy identification the appliances are marked with the symbol displaying a crossed out dustbin Centrifuges that are no longer in use and are ready for disposal contain...

Page 50: ...uare of the rotational speed and distance from the axis of rotation This force known as g force is quantified as the number of g applied to the sample No SI units are provided The following formula is...

Page 51: ...y connects by a line segment a speed to the relative centrifugal force RCF as a function of the radius of rotation selected In the example above the speed of 2500 rpm corresponds to a relative centrif...

Page 52: ...Originale Instructions Rev 1 3 User Maunal Loreena 50 68 8 3 Logbook Example of table to monitor the use of the centrifuge and accessories Date Operator RPM RCF Duration T Rotor Incidents...

Page 53: ...Designation Ref Catalog Serial No List of accessories attached to your equipment Reason for return Decontamination Agent used Duration of application Operator Remarks It is your responsibility to pro...

Page 54: ...xg AFI C300 Refrigerating 3500 rpm 2750 xg Buckets Part number NU B1000 Description 1 000 ml bucket Set of 4 Maximum speed 3500 rpm Maximum RCF 2750 xg Maximum capacity 1750 grams Lifetime Indicative...

Page 55: ...185182 mm 1030 23x Tubes 5 7 ml Round bottom 092 415CNU 196182 mm 2415Conical 23x Tubes 5 7 ml Round bottom 9692 410N 188182 mm 39x0 Blood Collection Tube39 156 45N 188182 mm 505 7Blood Collection Tub...

Page 56: ...number NU TXDW4 Description Stainless Steel Extractor Neoprene pad Set of 4 Configurations Loadings Each bucket must be loaded evenly Allowed Forbidden Loading the rotor The rotor must be loaded evenl...

Page 57: ...4731 xg Accessories Buckets Part number NU B750 Description Round bucket 750 ml Set of 4 Volume 750 ml Maximum capacity 1000 grams Maximum rotor speed 4600 rpm Maximum centrifugal force 4730 xg Lifeti...

Page 58: ...97 mm 7x 50 ml Tubes Conical bottom Without Lid bucket 28 AFI 3T50AOR 198 mm 6x AOR Tubes Advanced Oak Ridge 24 AFI 3T50S 197 mm 7x 50 ml Tube Skirted bottom Without Lid bucket 28 AFI 3T50SL 198 mm 1x...

Page 59: ...for NU RX750 rotor Set of 4 Maximum speed 4600 rpm Maximum RCF 4564 xg Microplate capacity 6 x 96 wells Deepwell block capacity 1 x 96 wells Maximum capacity 800 grams Lifetime Indicative duration 50...

Page 60: ...AFI C200 Ventilated 4500 rpm 3531 xg AFI C200 Refrigerating 5100 rpm 4537 xg Accessories for NU RHDW Buckets Part number NU BHDW Description Micro titration buckets Set of 2 Capacity standard Micropl...

Page 61: ...oplate carrier Capacity standard Microplates 56x 5 ml tubes Maximum capacity 1900 g Lifetime 30 000 cycles 6 years Radius 161 mm Adapter Sets of 2 adapters Pack of 4 matrix Part number AFI 3TD5 Descri...

Page 62: ...inale Instructions Rev 1 3 User Maunal Loreena 60 68 Configurations Loading the buckets Allowed Not allowed Loading the rotor Allowed Symmetrical loading Forbidden Incomplete loading Asymmetrical load...

Page 63: ...Originale Instructions Rev 1 3 User Maunal Loreena 61 68 Angular rotor NU RA24 2...

Page 64: ...mum capacity 8x 50 ml Conical tubes Maximum load 8 x 67 gr Micro tube tilt 25 Fastening ClickSpin system Maximum rotor speed 12100 rpm Maximum centrifugal force of the rotor 16660 xg Centrifuge perfor...

Page 65: ...rigerating 4800 rpm 3606 xg Maximum radius 140 mm Items included 30 Tube holders 30 Wells for round bottom tubes Accessories for NU RA30 15 Cups Part Number NU 3015R Description 15 ml round bottomed t...

Page 66: ...m rotor speed 13200 rpm Maximum centrifugal force of the rotor 21000 xg Centrifuge performance Maximum speed Maximum centrifugal force AFI C200 Ventilated 13200 rpm 21035 xg AFI C200 Refrigerating 132...

Page 67: ...m Maximum centrifugal force of the rotor 26620 xg Centrifuge performance Maximum speed Maximum centrifugal force AFI C300 Ventilated 14000 rpm 22132 xg AFI C300 Refrigerating 15500 rpm 27129 xg Maximu...

Page 68: ...m rotor speed 16000 rpm Maximum centrifugal force of the rotor 27720 xg Centrifuge performance Maximum speed Maximum centrifugal force AFI C200 Ventilated 15300 rpm 25350 xg AFI C200 Refrigerating 160...

Page 69: ...Originale Instructions Rev 1 3 User Maunal Loreena 67 68 10 EC Conformity Declaration...

Page 70: ...Originale Instructions Rev 1 3 User Maunal Loreena 68 68 Blank page...

Page 71: ......

Page 72: ...colas Copernic ZA Nord Bazouges 53200 CHATEAU GONTIER FRANCE T l 33 0 2 43 06 66 76 contact afigroups com www aficentrifuge com Non contractual photos All rights reserved including photos and illustra...

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