Gels
2-5
Setting Run Conditions
Selecting a Run
Mode
Use the following table to select the type of comb to use based on the
number of lanes you are running.
Note
The correct run mode is automatically chosen when the number of
lanes is selected.
Note
It is possible to run a gel of any number lanes in 96 Scan mode. There
will be the same number of data collection points per lane, but there will be an
area of blank space to the left and right of the samples due to extra scan width.
However, the Neural Net Tracker has been trained using gels run according to
the default parameters. (For example: 48-lane gels run in XL mode and 36 lane
gels run in Full Scan mode.) Any deviation from the default is likely to confuse
the tracker resulting in mistracked lanes.
Setting Run
Conditions
To set gel run conditions:
No. of Lanes
Comb
Run Mode
24
Shark’s-tooth
Full Scan
24
Square-tooth
Full Scan
32
Shark’s-tooth
Full Scan
34
Square-tooth
Full Scan
36
Shark’s-tooth
Full Scan
36
Square-tooth
Full Scan
48
Shark’s-tooth
XL Scan
50
Square-tooth
XL Scan
64
Shark’s-tooth
XL Scan
66
Square-tooth
XL Scan
96
Shark’s-tooth
96 Scan
Step
Action
1
Open the 377-96 Collection software.
2
Prepare a sample sheet as described in the ABI P
RISM
377 DNA
Sequencer User’s Manual.
IMPORTANT
Preparing a sample sheet prior to the run is
required for optimal tracker operation.
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