3-20
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Principles of Operation
Flow Cytometry
Section 3
RBC Parameters
Figure 3.9 RBC Data and Histogram
All numeric and frequency size distribution data are automatically displayed on the
RUN screen in the format selected. The size distribution data for the red cells is
displayed graphically as a histogram using 0° data. The size distribution data is
plotted on the X axis. The relative number of cells is normalized and plotted on the
Y axis. The RBC data are shown in Figure 3.9.
RBC Count
The
Red Blood Cell Count
is directly measured, and is expressed as follows:
RBC = # x M/µL
Counts below 1.0 x M µL are displayed to three decimal places. The RBC count is
corrected for coincidence and WBC interference.
MCV
The
Mean Cell Volume
is the average volume of the individual red blood cells.
The MCV is derived from the RBC size distribution data on the 0°, 10°, and 90°
histograms, and is expressed in femtoliters.
HCT
The
Hematocrit
is the ratio of red blood cells to plasma and is expressed as a
percentage of the whole blood volume. The HCT is calculated from the red blood
cell count and the mean cell volume as follows:
HCT = (RBC x MCV)/10