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AB Sciex API 5000 LC/MS/MS, Hardware Manual, Page: 5 / 16

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AB Sciex API 5000 LC/MS/MS Hardware Manual Download Page 5

Document Number: D1000092206 B

5 

1

System Overview

The API 5000™ LC/MS/MS system includes a triple quadrupole mass 

spectrometer, a Turbo V™ ion source, a computer, and the Analyst

®

 software.

Figure 1-1

API 5000™ system and bench

Principles of the System

This section describes features and applications of the API 5000™ system. 

Applications for the System

The API 5000 system is designed for quantitative analysis of small molecules.
This application involves measurement of specific molecular weight compounds, 

usually a drug or metabolite in urine or other body fluid, and their resulting 

WARNING! If you need to move the system, contact an FSE to assist you. Risk of 
personal injury or instrument damage

Note:

Before you operate the instrument, make sure you have read the Safety Practices 

guide.

Bench

Mass Spectrometer

Line Adjustment

Transformers

Roughing Pumps

Summary of Contents for API 5000 LC/MS/MS

Page 1: ...Hardware Guide API 5000 System D1000092206 B May 2010 ...

Page 2: ...r manufacturers and or their products which may contain parts whose names are registered as trademarks and or function as trademarks of their respective owners Any such usage is intended only to designate those manufacturers products as supplied by AB SCIEX for incorporation into its equipment and does not imply any right and or license to use or permit others to use such manufacturers and or thei...

Page 3: ...ow the Instrument Works 6 Parts of the Instrument 7 I O Panel 8 Gas and Vacuum Panel 8 Control Panel 9 Source Exhaust System 10 Vacuum Chamber 11 Shutting Down and Turning on the System 11 Source Gas Parameters 13 Turbo V Ion Source Settings 14 Compound Parameters 14 Related Documentation 14 Technical Support 14 Chapter 2 API 5000 Instrument Parameters 15 ...

Page 4: ...Contents 4 Document Number D1000092206 B ...

Page 5: ...ations for the System The API 5000 system is designed for quantitative analysis of small molecules This application involves measurement of specific molecular weight compounds usually a drug or metabolite in urine or other body fluid and their resulting WARNING If you need to move the system contact an FSE to assist you Risk of personal injury or instrument damage Note Before you operate the instr...

Page 6: ...of m z 5 1250 in quadrupole mode Enhanced resolution at reduced scan speeds For pharmaceutical and pharmacokinetic samples MS MS has the sensitivity and specificity needed to analyze hundreds of samples per day without requiring extensive sample preparation How the Instrument Works Mass spectrometry measures the mass to charge ratio of ions to identify unknown compounds to quantify known compounds...

Page 7: ... V source can use either the TIS TurboIonSpray probe or the APCI atmospheric pressure chemical ionization probe The instrument is configured to perform complex MS MS analysis but for less rigorous analytical requirements it can perform single MS scans Parts of the Instrument This section contains diagrams of the instrument and general descriptions of its components Figure 1 3 Front view Curtain Ga...

Page 8: ...ipherals and a sources connector for optional sources Figure 1 5 I O panel Gas and Vacuum Panel The vacuum line to the external roughing pumps is connected through the gas and vacuum panel through the port labeled backing pump The panel also houses the gas supply connections and the external connection for the source exhaust pump B B A A RF FEEDBACK RF FEEDBACK I O Panel Gas and Vacuum Panel Contr...

Page 9: ...uttons reset the two microprocessors on the system controller This has an effect similar to restarting the computer CAUTION Resetting the instrument using these buttons interrupts any scans and results in the loss of any unsaved files and data When the operational vacuum conditions are satisfied and the instrument is in analysis mode the Vacuum Status button is illuminated and the Fault button is ...

Page 10: ...source exhaust system is properly connected and functioning The exhaust pump draws the solvent vapors from the enclosed source housing and delivers them to a trap at the rear of the instrument chassis It is recommended that these vapors be passed through this trap and then vented to a fume hood or outside port F4 F3 F2 F1 POWER ON OFF VACUUM STATUS FAULT RESET WARNING see Note below Spare Output B...

Page 11: ...the ion detector Shutting Down and Turning on the System Use the following procedures if you need to shut down or turn on the system To shut down the system 1 In the Analyst software complete or stop any ongoing scans 2 Turn off the sample flow to the instrument WARNING Hazardous Materials Take all necessary precautions to ensure the safe disposal of the source exhaust gas Follow all applicable lo...

Page 12: ...er follow steps 5 to 7 CAUTION If the vacuum chamber is not going to be vented while the instrument is shut down we recommend the roughing pump remain turned on to prevent the pump exhaust from being sucked back into the vacuum chamber If you do not want to vent the vacuum chamber skip steps 6 and 7 5 Turn off the main power to the instrument As you face the instrument where the instrument name is...

Page 13: ...bulkhead 4 Turn on the main power switch 5 Make sure that the GPIB general purpose interface bus cable is connected to both the instrument and the computer 6 Turn on the computer if it was turned off and then start the Analyst software Source Gas Parameters The parameters in Table 1 1 are optimized for different LC conditions involving flow rate For more information about the parameters see the An...

Page 14: ...ress F1 Technical Support AB SCIEX and its representatives maintain a staff of fully trained service and technical specialists located throughout the world They can answer questions about the instrument or any technical issues that may arise For more information visit the web site at http www absciex com Table 1 2 Vertical and Horizontal Parameters Parameter Value X axis 5 Y axis 5 Table 1 3 Compo...

Page 15: ...00 4500 to 0 4500to 0 4500 to 0 NC 3 NC 3 3 3 3 3 3 3 0 to 5 0 to 5 0 to 5 5 to 0 5 to 0 5 to 0 TEM 2 3 TEM 2 3 0 0 0 0 0 0 0 to 750 0 to 750 0 to 750 0 to 750 0 to 750 0 to 750 OR DP 120 120 120 100 100 100 DP OR 0 to 400 0 to 400 0 to 400 400 to 0 400 to 0 400 to 0 Q0 EP 10 10 10 10 10 10 EP Q0 15 to 2 15 to 2 15 to 2 15 to 2 15 to 2 15 to 2 IQ1 IQ1 Q0 1 Q0 1 Q0 1 Q0 1 Q0 1 Q0 1 IQ1 Q0 offset 0 ...

Page 16: ... to 0 400 to 0 400 to 0 0 to 400 0 to 400 0 to 400 CEM CEM 2000 2000 2000 2000 2000 2000 500 to 3297 500 to 3297 500 to 3297 500 to 3297 500 to 3297 500 to 3297 GS1 GS1 20 20 20 15 15 20 0 to 90 0 to 90 0 to 90 0 to 90 0 to 90 0 to 90 GS2 GS2 0 0 0 0 0 0 0 to 90 0 to 90 0 to 90 0 to 90 0 to 90 0 to 90 ihe 4 ihe 1 1 1 1 1 1 0 or 1 0 or 1 0 or 1 0 or 1 0 or 1 0 or 1 IHT IHT 40 40 40 40 40 40 10 to 2...

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