A-M Systems 3000 Instruction Manual Download Page 4

A-M Systems

 

 

131 Business Park Loop, P.O. Box 850 Carlsborg, WA 98324

 

 

Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525

 

E-mail: [email protected] * Website: 

http://www.a-msystems.com

 

 

General Description 

Instrument Features

 

The 

AC/DC Differential Amplifier, Model 3000

 is a single-channel, differential amplifier 

that can be used with or without a head stage probe.  The instrument is designed for 
low-noise recordings from excitable tissue.  It is intended for extracellular recording 
and/or stimulating in conjunction with microelectrodes.  It can be used in a number of 
research or teaching applications requiring extracellular neurophysiological recording 
from excitable tissue, such as nerve, muscle (EMG), EEG, EKG, and ERG recordings.  

The instrument is not intended for clinical measurements using humans.

 

The 

Model 3000

 contains a high-gain, low-noise differential amplifier stage followed by 

low frequency, high-frequency, and notch filters.  Three operating modes are available 
to accommodate recording, stimulating, and verification of electrode impedance.  
Record Mode offers six levels of signal gain (x50, x100, x500, x1000, x5000, and 
x10,000).  Stimulus Mode allows the current passing through the electrode to be 
measured during stimulation at six levels of gain (5V/mA, 10V/mA, 0.05V/µA, 0.1V/ 
µA, 0.5V/µA, 1V/µA).  Impedance Mode utilizes an internally calibrated current source 
to allow in situ verification of electrode impedance and adjustment of capacitance 
compensation.  Units that are sold with a head stage are pre-configured to work with 
the headstage, and the front panel gain values include the gain of the headstage. 

Controls and Connectors

 

Input Controls 

PROBE:  

This 15-pin connector receives a signal either directly from the electrodes (non 

head stage version) or from the Head Stage probe for further processing. 

INPUT SELECT (DIFF-MONO-GND)

 

(non headstage version only)

:  

This switch sets the 

input for differential operation, monopolar operation, or grounds the inputs to the 
amplifier. 

INPUT LEADS

 (standard)

:  

Supplied with the Model 3000 is a DB-15 connector with three 

leads.  The black wire is the “+” electrode lead.  The white wire is the “-” reference 
lead. The silver wire is a driven shield, and 

should not be grounded! 

 This wire 

serves to minimize signal loss due to capacitance. 

HEADSTAGE PROBE

 (optional)

:  

The Headstage Probe has three sockets for connection  

to electrodes.  

PROBE+ 

is used to connect to the electrode (usually a high impedance 

recording microelectrode; It is the larger 2mm jack).  

PROBE- 

is used to connect to the 

indifferent lead.  Either the 

PROBE GND 

connector, or the front panel 

GND 

must be 

Summary of Contents for 3000

Page 1: ......

Page 2: ...L FOR AC DC DIFFERENTIAL AMPLIFIER MODEL 3000 Serial __________ Date____________ A M Systems PO Box 850 Carlsborg WA 98324 U S A 360 683 8300 800 426 1306 FAX 360 683 3525 http www a msystems com Version 7 0 March 2020 ...

Page 3: ...rocedure 6 Theory of Operation 9 Specifications 11 Warranty and Service 13 Each AC DC Differential Amplifier is delivered complete with One 3 Foot Cable Remote Power Supply Rack Mount Hardware NOTE This instrument is not intended for clinical measurements using human subjects A M Systems does not assume responsibility for injury or damage due to the misuse of this instrument ...

Page 4: ...rode to be measured during stimulation at six levels of gain 5V mA 10V mA 0 05V µA 0 1V µA 0 5V µA 1V µA Impedance Mode utilizes an internally calibrated current source to allow in situ verification of electrode impedance and adjustment of capacitance compensation Units that are sold with a head stage are pre configured to work with the headstage and the front panel gain values include the gain of...

Page 5: ...rent through the electrode while the unit is in stimulate mode STIM The center pin of the BNC is the input and the outer conductor is the stimulus input When the unit is in the STIM mode the output will be a measure of the current passing through the electrode in six ranges 5V mA 10V mA 50mV µA 0 1V µA 0 2V µA or 1V µA STIM GATE REC This toggle switch controls the operating mode for the Model 3000...

Page 6: ...ts the variable DC offset voltage which is summed with the input voltage This feature may be used to compensate for electrode potentials and to position the signal trace on an oscilloscope recording device An input offset range of 0 0 V to 250 mV is available The COARSE knob will cover 250 mV and the FINE knob will add up to 25 mV to the COARSE knob s position DC OFFSET SWITCH OFF This switch sets...

Page 7: ...ASS switch is set at 1 kHz then a 10 kHz signal will be attenuated by a factor of 100 while a 100 kHz signal will be attenuated by a factor of 10 000 One of the uses of this filter is to reduce high frequency noise that is above the frequency content of the signal being recorded Head stage indicator HEAD STAGE This LED indicates whether the unit is configured for use with HEAD STAGE lit or without...

Page 8: ...than the model 3000 you will need to complete the following procedure To set the model 3000 for use with a head stage all jumpers must be set to the H position see figure 2 below Once the jumpers are in the H position the unit can only be used with a head stage To return the unit for use without a head stage simple move the jumpers to their previous position Calibrate the unit for headstage Set th...

Page 9: ...OBE GND or the GND on the front panel must be connected to provide a return current path If neither ground is connected the Head Stage Amplifier will saturate and no signal will be measured Care should be taken to keep the power cables from all instruments as far away as possible from the recording setup One exception is the DC power cable that supplies power to the model 3000 This side of the cab...

Page 10: ...T BNC with the horizontal sweep rate set to 2ms division Zero the scope Flip the input switch to DIFF 7 Observe the offset potential between the two electrodes on the oscilloscope Set the DC OFFSET OFF switch to the appropriate polarity and adjust the DC OFFSET knob to zero the amplifier output Note make sure your oscilloscope is in DC mode 8 Turn on the Ω TEST button to inject a 100 Hz square wav...

Page 11: ... lead is equal to and opposite of that in the active lead only isolated stimulus sources should be used An added benefit of isolated stimulus sources is that they produce less stimulus artifact than ground referenced sources The Positive Conductor of the Stimulus connector center pin is connected through the Probe to the active electrode lead while the Negative Conductor outside ring of the BNC is...

Page 12: ...he signal from this amplifier is coupled through positive feedback with a capacitor to form capacity compensation The capacity compensated signal is offset adjustable through a precision variable voltage reference The signal from the initial stage then passes through High pass if activated Notch if activated and Low pass filters Finally the signal passes through a gain stage of up to 200 times The...

Page 13: ...OBE the indifferent electrode lead At the same time relay K1 to connect the stimulus source at the STIMULUS INPUT connector to the electrode leads A stimulus current from an isolated source passes through the PROBE lead to the active electrode returning through the PROBE lead and the 100Ω resistor The current creates a voltage across the 100Ω resistor which is amplified by the differential amplifi...

Page 14: ... 10V Gain 250mV input offset adjust Stability versus temperature 50µV ºC Stability versus time Stimulus Mode 1mV 12hours Output current ratio 5V mA 10V mA 50mV µA 0 1V µA 0 2V µA 1V µA Maximum output current reading 10V or 2mA Maximum stimulus current Maximum stimulus voltage 10mA Diff 100V Diff Impedance Input 15V Output impedance ratio 2nA source 0 1Vp p MΩ 0 2Vp p MΩ 1Vp p MΩ 2Vp p MΩ 10Vp p MΩ...

Page 15: ... 10V minimum High Pass filter settings DC 0 1 1 10 100 300 Hz 15 High Pass filter gain 40dB decade Low Pass filter settings 0 1 0 3 1 3 10 20 kHz 15 Low Pass filter gain Power 40dB decade REMOTE AC Power source 100 240 VAC 50 or 60 Hz DC voltage into the amplifier for use inside of faraday cage Physical Dimensions Width 8 5 inches 21 6 cm Height 4 inches 10 2 cm Depth 3 5 inches 8 9 cm Weight 5 po...

Page 16: ...e the obligations of A M Systems under this warranty During the warranty period A M Systems agrees to repair or replace at its sole option without charge to the Purchaser any defective component part of the hardware To obtain warranty service the Purchaser must return the hardware to A M Systems or an authorized A M Systems distributor in an adequate shipping container Any postage shipping and ins...

Page 17: ...ought against you by another party THIS EQUIPMENT IS NOT INTENDED FOR CLINICAL MEASUREMENTS USING HUMAN SUBJECTS A M SYSTEMS DOES NOT ASSUME RESPONSIBILITY FOR INJURY OR DAMAGE DUE TO MISUSE OF THIS EQUIPMENT Jurisdictions vary with regard to the enforceability of provisions excluding or limiting liability for incidental or consequential damages Check the provision of your local jurisdiction to fi...

Page 18: ...iption 2 6 30 06 Initial Document Control release 3 4 28 10 DCR201200 Warranty and Company info 4 11 20 15 DCR 202472 202512 Correct for x10Hs 5 2 27 17 DCR 202739 Correct Rev Numbering 6 1 18 19 DCR 202615 Review content add content rev control 7 3 19 20 DCR 203316 Update Warramty 8 ...

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