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ZEISS
Center Screen Area / Image Containers - Display and Image Analysis
ELYRA 7
398
000000-2262-999
03/2019 V_02
Associated with the Molecular Density map is a Scale
bar that shows the color code of
Molecule Density
in Molecules /
µ
m
2
according to the
HR
channel
assigned look up table (LUT) (Fig. 668).
Please note that molecular density applies for a
plane only. Hence, for a 3D stack, a map for
each slice is available. There is no density
mapping in z-direction.
The available functionalities will alter with the
Display Mode
chosen (Fig. 669, Fig. 670, Fig. 671
and Fig. 673). Only the
Pixel Resolution [nm/pixel]
slider and the
Display Raw Image
are available for
each
Display Mode
.
For all
Display Modes
displaying a centroid or cross
(
Centroid
,
Gauss + Centroid
,
Cross
,
Gauss +
Cross
) the
Display Mode Color
drop down menu is
available (Fig. 670, Fig. 671), from which the color of
the overlay (centroid or cross) can be selected
(Fig. 672).
For all
Display Modes
displaying a Gauss rendered
peak / object (
Gauss
,
Gauss + Centroid
,
Gauss +
Cross
) the
Expansion factor [x PSF]
slider is
available (Fig. 669, Fig. 671). The number set with the
slider or by using the input spin boxes will be
multiplied with the PSF (localization precision error),
so the rendered peaks / objects become smaller for
numbers < 1 and bigger for numbers > 1.
Fig. 668 Display Mode for Molecular density
with part of the map (left) and scale bar
(right)
Fig. 669 Appearance of the SMLM-Render view
control tab with Display Mode Gauss
selected (for 2D SMLM data set)
Fig. 670 Appearance of the SMLM-Render view
control tab with Display Mode Centroid
(or Cross) selected (for 2D SMLM data
set)
Fig. 671 Appearance of the SMLM-Render view
control tab with Display Mode
Gauss+Centroid (or Gauss + Cross)
selected (for 2D SMLM data set)
Fig. 672
Display Mode Color drop
down menu