Zeiss Axioscope 7 Скачать руководство пользователя страница 97 | Manualshive

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Zeiss Axioscope 7 Скачать руководство пользователя страница 97

OPERATION

Axioscope 5/7/Vario

Illumination and contrast methods in transmitted light …

ZEISS

03/2018

430035-7344-001

97

4.2.5

Setting up transmitted light PlasDIC contrast microscopy

(1) General principle of operation

PlasDIC can be used independently from the material of the specimen holder.
The contrast method gives a relief-like image and is especially well suited for thicker objects. The contrast
is adjustable. It is possible to contrast the cavities of microtiter plates up to the edge. It is not necessary to

use cultivation holders with a glass base.

(2) Instrumentation

−

Abbe condenser with modulator disk and objective-dependent 2 mm slit diaphragm (A-Plan 10x

and LD A-Plan 20x) or 4.5 mm slit diaphragm (in all other cases)

−

Objectives:

A-Plan 10x, 20x, 40x;

LD A-Plan 20x, 32x, 40x;

LD Plan-Neofluar 20x, 40x, 63x

−

PlasDIC slider depending on the objective

−

ACR P&C analyzer module for transmitted light and reflector turret /slider or D/A fixed or rotatable

analyzer slider in connection with mounted intermediate plate for analyzer slider 12x46

(3) Setting PlasDIC

•

Fully open the aperture on the condenser.

•

Place the sample on the stage.

•

Swing the position with the 2 or 4.5 mm slit diaphragm for PlasDIC of the condenser into the beam

path. Increase brightness when switching from brightfield to PlasDIC.

•

Swing the analyzer module into the beam path or slide analyzer slider in beam path in intermediate

plate.

•

Swing in the objective for PlasDIC.

•

Slide the PlasDIC slider on the nosepiece into the DIC slit of the objective in use.

•

Adjust the contrast with the knurled screw of the PlasDIC slider. You can now see the structures in

relief or in pseudo-darkfield. The best result is achieved in the relief mode.

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Содержание Axioscope 7

Страница 1: ...Operating Manual Axioscope 5 7 Vario Upright Microscope for Routine and Entry level Research Microscope for Routine and Entry Level Research...

Страница 2: ...d including rights created by patent grant or registration of a utility model All names of companies and products mentioned in this manual may be trademarks or registered trademarks Third party produc...

Страница 3: ...2 Binocular ergo tube ergo photo tube 20 23 and ergo photo tube 15 23 each with continuous vertical adjustment 37 2 5 3 Nosepiece with objectives 38 2 5 4 Mechanical stage 75x50 38 2 5 5 Low power sy...

Страница 4: ...ication changer 73 3 2 4 Changing the filters in the reflector module FL P C 74 3 2 5 Changing the color splitter in the reflector module FL P C 75 3 2 6 Mounting the polarizer D or the filter holder...

Страница 5: ...112 4 3 1 Setting up reflected light brightfield microscopy using the K HLER method 112 4 3 2 Setting the reflected light darkfield 115 4 3 3 Setting reflected light DIC and reflected light C DIC 116...

Страница 6: ...10 Binocular ergo photo tube 15 23 telescopic with vertical adjustment 37 Fig 2 11 Nosepiece with DIN slot for compensators 38 Fig 2 12 Mechanical stage with specimen holder 38 Fig 2 13 Low power sys...

Страница 7: ...FL P C 74 Fig 3 42 Mounting the filters and the color splitter 74 Fig 3 43 Opening the module 75 Fig 3 44 Changing the color filter 75 Fig 3 45 Labeling the color splitter 76 Fig 3 46 Mounting the po...

Страница 8: ...for circular polarization contrast 105 Fig 4 13 Determining the optical character 110 Fig 4 14 External power supply unit for HAL 100 112 Fig 4 15 Setting the microscope in the reflected light bright...

Страница 9: ...e operator must comply The following warning instructional and informational symbols are used in the manual CAUTION Warns the operator of a possible danger CAUTION Warns the operator of hot surfaces C...

Страница 10: ...DC 100 W external power supply unit for HAL 100 covers a voltage range from 100 to 127 V AC and 200 to 240 V AC 10 and a frequency range of 50 to 60 Hz It adapts automatically to the applied voltage...

Страница 11: ...ope without the necessary safety equipment e g special damping filters or the fluorescence protection screen Gas discharge lamps have a high internal pressure when hot Never change the lamps while the...

Страница 12: ...ION The manufacturer assumes no liability for any use of the microscope its assemblies or individual parts if these are used in in a manner or for any purpose other than those described in this operat...

Страница 13: ...ing the HAL or HBO illuminators a base plate for the Axioscope 000000 2202 526 is mandatory ATTENTION Do not dispose of defective microscopes in the household waste Comply with the applicable statutor...

Страница 14: ...icroscopes Axioscope 5 7 Vario 14 430035 7344 001 03 2018 Fig 1 2 Warning labels on microscopes with HAL 100 for transmitted light and HBO 100 for reflected light Fig 1 3 Warning label on microscopes...

Страница 15: ...ng labels on the microscopes ZEISS 03 2018 430035 7344 001 15 Fig 1 4 Warning label on microscopes with Colibri 3 light source for reflected light Fig 1 5 Warning label on Axioscope 7 stand using the...

Страница 16: ...arts and to improper handling are not covered by our warranty The manufacturer is not liable for damage to the instrument due to incorrect operation negligence or any other manipulation of the instrum...

Страница 17: ...n aerospace and metallographic laboratories quality assurance and control in the automobile and mechanical engineering industries failure analysis in the electronics and semiconductor industries The a...

Страница 18: ...emperature 10 to 40 C Permissible relative humidity no condensation max 75 at 35 C Highest permitted altitude of use max 2000 m Air pressure 800 hPa to 1060 hPa Degree of pollution 2 Operational speci...

Страница 19: ...light Power consumption max 10 W Adjustment of light source continuous approx 10 to 800 mA Halogen lamp 12 V 50 W Adjustment of light source infinitely variable from approx 3 to 12 V Halogen lamp 12...

Страница 20: ...io 20 430035 7344 001 03 2018 2 3 Interface diagram The following figure shows a diagram of the microscope s hardware interfaces The Axioscope 5 stand Bio TL RL with LED HAL illumination serves as the...

Страница 21: ...l for transmitted light 10 Condenser carrier 11 Condenser 12 Stage carrier 13 Stage 2 4 Controls and functional elements on the microscope 2 4 1 Stand models Eight stand models are available in the de...

Страница 22: ...photo tube with mounted Axiocam 208 optionally 4 Transmitted light stand 5 Transmitted light illuminator 6 Intensity LM knob for light intensity and Light Manager function LM 7 Indicator light for tra...

Страница 23: ...RIPTION OF THE INSTRUMENT Axioscope 5 7 Vario Controls and functional elements on the microscope ZEISS 03 2018 430035 7344 001 23 Fig 2 2 Controls and functional elements of the Axioscope 5 stands Bio...

Страница 24: ...lider for reflected light 8 Reflected light illuminator 9 Transmitted and reflected light stand 10 Intensity LM knob for light intensity and Light Manager function LM 11 Transmitted light illuminator...

Страница 25: ...IPTION OF THE INSTRUMENT Axioscope 5 7 Vario Controls and functional elements on the microscope ZEISS 03 2018 430035 7344 001 25 Fig 2 3 Controls and functional elements of the Axioscope 5 stand Bio T...

Страница 26: ...tube part 3 Binocular tube photo tube with mounted Axiocam 208 optionally 4 Slot for polarizer slider A 60x30 mm 5 Field diaphragm as slider 6 Aperture diaphragm as slider 7 Filter slider for reflect...

Страница 27: ...IPTION OF THE INSTRUMENT Axioscope 5 7 Vario Controls and functional elements on the microscope ZEISS 03 2018 430035 7344 001 27 Fig 2 4 Controls and functional elements of the Axioscope 5 stand Pol T...

Страница 28: ...der 7 Filter slider for reflected light 8 Reflected light illuminator 9 Transmitted and reflected light stand 10 Intensity LM knob for light intensity and Light Manager function LM 11 Transmitted ligh...

Страница 29: ...IPTION OF THE INSTRUMENT Axioscope 5 7 Vario Controls and functional elements on the microscope ZEISS 03 2018 430035 7344 001 29 Fig 2 5 Controls and functional elements of the Axioscope 5 stand Mat T...

Страница 30: ...for polarizer slider A 60x30 mm 5 Field diaphragm as slider 6 Aperture diaphragm as slider 7 Filter slider for reflected light 8 Reflected light illuminator 9 Transmitted and reflected light stand 10...

Страница 31: ...TION OF THE INSTRUMENT Axioscope 5 7 Vario Controls and functional elements on the microscope ZEISS 03 2018 430035 7344 001 31 Fig 2 6 Controls and functional elements of the Axioscope 7 stand Mat TL...

Страница 32: ...crank for vertical adjustment 8 Stage carrier and base plate Key to Fig 2 7 1 Eyepieces 2 Binocular tube part 3 Binocular tube photo tube with mounted Axiocam 208 optionally 4 Release lever for vertic...

Страница 33: ...TION OF THE INSTRUMENT Axioscope 5 7 Vario Controls and functional elements on the microscope ZEISS 03 2018 430035 7344 001 33 Fig 2 7 Controls and functional elements of the Axioscope 5 Vario materia...

Страница 34: ...hort press Switches RL TL illumination alternately on off The respective indicator light is continuously illuminated Second press to the RL TL button to turn off on the illumination the indicator ligh...

Страница 35: ...on the left see Fig 2 6 28 Yes No No Short press Switches between XY stage control and Z axis control via focus drives If Z axis control is active the indicator light lights in GREEN the left or righ...

Страница 36: ...ol 100 light to the eyepieces Shift knob 712HFig 2 8 3 in back position camera symbol 100 light to the camera Push pull rod 713HFig 2 8 1 pushed in Eyepiece shutter is closed Push pull rod 714HFig 2 8...

Страница 37: ...if needed The ergo tubes permit a height adjustment range of 44 mm A larger range can be obtained by swiveling the binocular section from the lower to the upper observation position depending on the i...

Страница 38: ...ns 704HFig 2 11 1 depending on the configuration Turning the knurled wheel of the nosepiece 705HFig 2 11 2 enables you to change the objectives quickly 2 5 4 Mechanical stage 75x50 Mechanical stage 71...

Страница 39: ...n securely Do not use the low power system with LED illumination for transmitted light ATTENTION If the condenser carrier with mounted low power system is moved too far down the low power system may c...

Страница 40: ...uired to function as a field diaphragm F and another to function as the aperture diaphragm A To open the diaphragm turn the knurled wheel 724HFig 2 15 1 up To close the diaphragm turn the knurled whee...

Страница 41: ...we advise that you write down the appropriate data for each position of the mounted reflector modules on the adhesive labels provided with the instrument Then attach the labels at the appropriate poin...

Страница 42: ...d type However they apply analogously to other microscope stand models Special features are described separately The same activities apply when assembling a microscope stand with an upper stand part a...

Страница 43: ...th gear box 288HFig 3 2 3 and the Vario stand column 288HFig 3 2 1 proceed as follows Unpack the Vario upper stand part with gear box and the Vario stand column Position the Vario upper stand part 287...

Страница 44: ...n the Allen screws with the Allen wrench 3 1 4 Inserting eyepieces or auxiliary microscope or pinhole diaphragm Remove both dust cover caps 299HFig 3 4 1 and 5 from the binocular tube Remove both eyep...

Страница 45: ...be used with eyepieces Fig 3 6 3 marked with a red dot Fig 3 6 R Unscrew mounting stop Fig 3 6 6 from the eyepiece Fig 3 6 3 Pull locking ring Fig 3 6 4 out Insert eyepiece reticle Fig 3 6 5 into the...

Страница 46: ...the stage carrier to the bottom stop or lower the stage carrier with the dovetail guide Remove the dust protection caps 310HFig 3 7 2 from the appropriate nosepiece openings Take the objective 311HFi...

Страница 47: ...t aside with the top side facing down Insert the module 317HFig 3 8 3 with the top side facing down with the aid of its mounting elements on its left and right 318HFig 3 8 4 at a slant from the top in...

Страница 48: ...Mounting the mechanical stage Depending on the type the mechanical stages can either be stable or they can be rotated and centered The shift range is 75 mm in the X direction and 50 mm in the Y direct...

Страница 49: ...rrected easily as described below c How to adjust the X and Y axis knob tension In the X direction hold the specimen holder and move the stage guide to the left and right until it hits the stoppers Fi...

Страница 50: ...e Loosen the clamping screws of the stage 343HFig 3 13 4 and the screw cap of the stage carriers 344HFig 3 13 1 Determine the maximal shift of the specimen 345HFig 3 13 5 arrow point to the cross line...

Страница 51: ...he smoothness of ergonomic operation is factory pre set to a medium level The operator can change the friction as follows a How to adjust the X direction Remove the additional sleeves 353HFig 3 14 1 a...

Страница 52: ...pin 362HFig 3 15 5 out of the coaxial knurled knob for the Y adjustment 363HFig 3 15 1 and stick it into one of the holes on the lower hole nut 364HFig 3 15 3 Hold the coaxial knurled knob for the X...

Страница 53: ...3 16 8 3 1 10 2 Attaching the Pol rotary stage If necessary loosen the screw cap Fig 3 16 8 of the spring pin with about 3 turns Place the Pol rotary stage with the groove of the dovetail stage bottom...

Страница 54: ...lamping screw accessible 3 1 10 5 Centering the Pol rotary stage With high power objectives stage centricity can be exact only for one selected objective All stages are factory pre centered i e when r...

Страница 55: ...ing use a contrast rich specimen and an eyepiece with reticle First turn the nosepiece to swing the non centerable objective mount mount with DIC slot into the light path Center the rotary stage for t...

Страница 56: ...the four mounting screws Fig 3 19 5 with the Allen wrench AF 3 327HFig 3 19 4 Carefully remove the stage from the carrier How to mount the stage Fig 3 20 Plugging in the power cable of the motorized m...

Страница 57: ...al adjusting range is usable Slide the condenser carrier 397HFig 3 22 3 with the knurled knob for vertical adjustment 398HFig 3 22 2 all the way down Open the front lens if switchable on the condenser...

Страница 58: ...possible relative to the stage opening 3 1 14 Mounting the darkfield condenser NOTE The condenser holder Z for darkfield is required to place darkfield condensers in the condenser carrier Insert the...

Страница 59: ...xtension for 30 mm is mounted Opening with mark 60 408HFig 3 24 1 The sample space extension for 60 mm 430030 9111 000 is mounted Insert the stage carrier 409HFig 3 24 5 at a slight angle beneath the...

Страница 60: ...ed 12 V 50 W halogen lamp if necessary by pulling it out of the cap 413HFig 3 26 2 in an upward motion Push the new lamp 414HFig 3 26 1 with its two cap pins gently and carefully into the lamp cap 415...

Страница 61: ...ation connector Fig 3 27 2 at the lower back of the stand Connect the illuminator s power plug Fig 3 27 4 to the socket Fig 3 27 3 on the lower part of the stand or the upper part of the stand Match t...

Страница 62: ...ators a base plate for Axioscope 000000 2202 526 is mandatory Fig 3 28 Mounting the base plate Remove the four rubber feet Fig 3 28 1 from the bottom of the stand Align the base plate Fig 3 28 2 with...

Страница 63: ...29 2 or 420HFig 3 29 3 Now fixate it by tightening the clamping screw Fig 3 29 1 or 422HFig 3 29 8 with the Allen wrench NOTE Due to the lack of ground clearance it is important to avoid tilting the...

Страница 64: ...both images of the lamp filament appear as sharp as possible on the projection surface Adjust the adjustment screws 433HFig 3 30 4 and 5 so that the lamp filament of one image exactly fills the gaps...

Страница 65: ...aside Press both spring levers downwards 443HFig 3 31 5 and pull out the old halogen lamp 444HFig 3 31 6 in an upward motion Press both spring levers downwards insert the new lamp into the lamp cap 44...

Страница 66: ...tool is used to adjust the HBO 100 illuminator see section 3 1 21 3 3 1 21 Mounting and adjusting the HBO 100 illuminator 3 1 21 1 Inserting the HBO 103 W 2 mercury vapor short arc lamp For safety re...

Страница 67: ...dovetail ring in the reflected light connection piece 476HFig 3 33 1 on the back of the unit Tighten the clamping screw 477HFig 3 33 2 with the Allen wrench AF 3 Connect the HBO 100 lamp cable to the...

Страница 68: ...0 481HFig 3 55 1 and wait until it reaches its operational temperature Pull the adjustment tool 482HFig 3 35 1 out of the microscope stand The lighter colored focal point of the lamp HBO 103 W 2 and i...

Страница 69: ...ower is switched off Never look directly into the light emitting aperture of the lamp With the Allen wrench AF 3 Fig 3 37 6 loosen the clamping screw Fig 3 37 5 of the RL illumination connector on the...

Страница 70: ...ig 3 38 1 4 Select the right LED module with matched LED specific labels Fig 3 38 1 2 and 1 5 and insert it in the correct slot Fig 3 38 NOTE One label on the LED slot Fig 3 38 1 5 indicates the wavel...

Страница 71: ...52 9573 000 505 nm LED 423052 9562 000 565 nm LED 423052 9602 000 625 nm LED 423052 9522 000 Slot 1 450 480 nm X O X X X Slot 2 350 415 nm O X X X X Slot 3 594 660 nm X X X X O Slot 4 508 565 nm X X O...

Страница 72: ...d ring tool to unscrew the tube lens accessible from the bottom and keep it free of dust Screw the tube lens included with the intermediate plate into the tube Insert the intermediate plate 514HFig 3...

Страница 73: ...nger Remove the camera adapter or the dust protection cover from the camera port 521HFig 3 40 2 of the photo tube Mount the magnification changer 522HFig 3 40 1 on the camera port adjust it and fasten...

Страница 74: ...indicates the direction of insertion and must always point to the inside of the reflector module see arrows in 532HFig 3 41 To minimize the image offset in multiple fluorescence images the emission f...

Страница 75: ...ver Hold both parts of the reflector module the emission part 542HFig 3 43 2 and the excitation part 543HFig 3 43 3 together turn them against the mounting position and put them down Now tilt the exci...

Страница 76: ...or easy assembly remove the condenser carrier 557HFig 3 46 3 fully with the stage carrier If necessary unscrew stop bolt and bracket bolt with low power system from the condenser carrier Hold the pola...

Страница 77: ...7 into the front threaded opening to the left below the condenser carrier 571HFig 3 47 3 until it stops With the adjustment lever 572HFig 3 47 1 screw the stop bolt 573HFig 3 47 2 into the rear threa...

Страница 78: ...ping screw 582HFig 3 48 5 of the condenser s dial segment 583HFig 3 48 3 with the Allen wrench AF 3 and pull out the dial segment Slide the modulator disk 584HFig 3 48 4 with its two pronged forked op...

Страница 79: ...e orientation grooves 593HFig 3 49 6 Screw in the adapter ring 594HFig 3 49 8 included with the slit diaphragm with the aid of the appropriate tool 595HFig 3 49 1 3 2 10 Changing the PhC DIC PlasDIC d...

Страница 80: ...the socket out of the stand base If necessary pull the filter to be changed 602HFig 3 51 1 out of the corresponding position of the filter wheel 603HFig 3 51 2 Put the new filter into the position NOT...

Страница 81: ...supply 486HFig 3 52 2 with its power cord to an electrical outlet NOTE The microscope can adjust to power voltage and frequency ranges of 100 to 240 V AC 50 60 Hz The power supply unit adjusts automat...

Страница 82: ...ing port Remote on the back of the stand or to that of the power supply unit Fig 3 54 4 Switch the toggle switch Fig 3 53 3 for reflected or transmitted light to the TL position transmitted light 3 3...

Страница 83: ...ght switch the toggle switch Fig 3 53 3 for reflected or transmitted light to the TL position for transmitted light or to the RL position for reflected light Switch the HBO 100 illuminator for overhea...

Страница 84: ...ave the light intensity using the LM function Press the Intensity LM knob for at least 1 5 seconds The currently set light intensity value will be saved After it is saved the active LED illuminator sw...

Страница 85: ...y one round image when looking into the eyepieces 4 1 2 Setting the viewing height Adjust the viewing height to your individual requirements by swiveling the eyepiece tubes up 606HFig 4 2 A or down 60...

Страница 86: ...es of ametropia follow the subsequent steps Focus on the line figure of the eyepiece reticle with the focusable eye lens of the adjustable eyepiece Focus on the microscopic image of a loaded specimen...

Страница 87: ...t brightfield microscopy Every microscope except the one with the Vario stand column is configured to work with the transmitted light brightfield method All available condensers except special condens...

Страница 88: ...he best contrast at almost full resolution and is thus the best compromise for the human eye Replace the eyepiece in the tube barrel NOTE Each objective changes the size of the object field and the ob...

Страница 89: ...vertical adjustment on the condenser by small increments only to avoid lifting out the sample Tighten the setscrew 627HFig 4 4 1 on the height stop 5 Adjusting the height stop on the focusing drive T...

Страница 90: ...rom aplan 0 9 BF DF PhC DIC Darkfield condenser with dry darkfield 465505 0000 000 applicable aperture from 0 6 0 75 Ultra condenser 465500 0000 000 applicable aperture from 0 75 1 3 Setting up transm...

Страница 91: ...n until the end stop Place the dry darkfield condenser in the condenser holder Z see section 3 1 14 Place the condenser holder Z in the condenser carrier and center it roughly so the condenser fits in...

Страница 92: ...rrier with the adjustment screws and focus the image with the condenser drive Place a drop of immersion oil on the specimen holder swivel in immersion oil objective and focus the specimen Close the fi...

Страница 93: ...e intermediate image plane the partial beams are thus differently affected and achieve interference and strengthen or weaken each other constructive and destructive interference depending on their pha...

Страница 94: ...4 7 B Remove the auxiliary microscope from the tube and replace it with the eyepiece To increase the image contrast a green 32 x 4 interference broadband filter may be mounted on the field diaphragm...

Страница 95: ...DIC Analyzer module ACR P C for transmitted light in the reflector turret slider or analyzer slider D A fixed or rotatable in conjunction with a mounted intermediate plate for analyzer slider 12x46 R...

Страница 96: ...ansmitted light Axioscope 5 7 Vario 96 430035 7344 001 03 2018 1 DIC slider 2 Knurled screw 3 Objective on the nosepiece 4 Condenser with DIC prism 5 Compensator 6 Analyzer module 7 Reflector turret F...

Страница 97: ...20x 40x LD A Plan 20x 32x 40x LD Plan Neofluar 20x 40x 63x PlasDIC slider depending on the objective ACR P C analyzer module for transmitted light and reflector turret slider or D A fixed or rotatabl...

Страница 98: ...d if no polarizer is already integrated in the condenser ACR P C analyzer module for transmitted light in the reflector turret slider or D analyzer slider fixed or with lambda plate Depolarizer for av...

Страница 99: ...pty position must be swiveled in on the reflector turret slider Bring the specimen to be examined into the field of view and turn it with the rotary stage Normally birefringent anisotropic objects wil...

Страница 100: ...ing a condenser with an integrated polarizer ACR P C analyzer module for transmitted light in the reflector turret slider or analyzer slider D fixed or with lambda plate Pol adjusting tool sample for...

Страница 101: ...tion The sample can have any color in this position Slide in the compensator Like the sample the compensator is a birefringent object but it has a defined path difference of 550 nm and a maximum oscil...

Страница 102: ...nvestigated is parallel to the largest polarization direction of the compensator i e in the NE SW direction the path difference of the sample i e gray white 150 nm and the path difference of the compe...

Страница 103: ...out 0 2 Rotate the Pol rotary stage until the specimen is in extinction position i e it appears entirely dark In this position activate the 45 click stop Rotate the stage once by 45 so that the specim...

Страница 104: ...on the condenser including the corresponding 4 plate analyzer slider or D analyzer module in the reflector turret 3 Setting the microscope Set the microscope as for transmitted light brightfield micr...

Страница 105: ...pecimen unless the above adjustment has been done Place the specimen to be examined on the stage The specimens appear constantly and independently of the stage rotation in their specific interference...

Страница 106: ...t path the aperture diaphragm fully open and the objective too should be a high aperture type 4 2 7 1 Application The determination of the optical character of transparent and weakly absorbent crystal...

Страница 107: ...y For conoscopy of small crystals close the luminous field diaphragm if necessary to prevent the axial figure of the examined crystal from being superimposed by the axial figures of adjacent crystals...

Страница 108: ...ut the specimen onto the stage and focus on it In the case of uniaxial crystals the most favorable orientation for conoscopic viewing is obtained with those specimen features e g of a thin section tha...

Страница 109: ...light brightfield microscopy using the K HLER method see also section 4 2 1 Put the specimen onto the stage and focus on it Move the polarizer and the D analyzer module into the light path First alig...

Страница 110: ...arranged colored interference fringes the so called isochromates might appear see also Fig 4 13 second row The lines of this black cross remain closed when you rotate the stage It can be located insi...

Страница 111: ...straight and run related to the reticle parallel through the pupil the specimen is optically uniaxial If the black isogyres are curved lines traveling across the pupil on a circular path the specimen...

Страница 112: ...s the microscopic intermediate image This image can then be examined visually or documented objectively 2 Instrumentation Axioscope with pre installed LED 10 W illuminator or optionally used with HAL...

Страница 113: ...iew 668HFig 4 15 D To adjust the aperture diaphragm image contrast remove an eyepiece from the tube barrel and look into the barrel or mount an auxiliary microscope instead of the eyepiece Center the...

Страница 114: ...14 430035 7344 001 03 2018 1 Centering screw of the aperture diaphragm 2 Knurled button of the aperture diaphragm 3 Centering screw of the field diaphragm 4 Knurled wheel of the field diaphragm 5 Focu...

Страница 115: ...tives Epiplan Neofluar EC Epiplan Neofluar Epiplan with the additional label HD Darkfield ACR P C reflector module for reflected light in the reflector turret slider 3 Setting the reflected light dark...

Страница 116: ...Adjust the microscope for reflected light brightfield microscopy as described in section 4 3 1 Open the field diaphragm enough so that the edge of the diaphragm lies just barely outside of the edge o...

Страница 117: ...scribed in section 4 3 1 Swing the C DIC P C reflector module on the reflector turret into the beam path Slide the 6x20 mm TIC slider in the compensator slot 6x20 mm 679HFig 4 16 1 You will see chroma...

Страница 118: ...l point of their wave lengths The measured path difference depends on the aperture and increases with the illumination aperture The following correction values must be considered depending on the obje...

Страница 119: ...with a homogeneous layer and the path differences are measured If the layers and the steps are transparent as with silicon dioxide on silicon for example the interference stripes can change their colo...

Страница 120: ...ons which are inevitable when working with objectives with very low scale figures 2 Instrumentation Axioscope with pre installed LED 10 W illuminator or optionally used with HAL 100 halogen lamp mount...

Страница 121: ...he stage rotates For samples with low bireflectance we recommend using the analyzer with a rotatable lambda plate Pleochroism is present when the color of the sample changes as soon as the stage rotat...

Страница 122: ...ether with the according dichroitic beam splitter 2 Instrumentation Appropriate objectives e g EC Plan Neofluar or Fluar UV excitation FL P C reflector module and safety shutter in the upper part addi...

Страница 123: ...Colibri 3 together The indicator light of the respective LED module on the Colibri 3 lights up when this module is switched on NOTE If using the HBO 100 illuminator switch on the external power suppl...

Страница 124: ...iew Using both centering screws 696HFig 4 20 5 center the field diaphragm on the edge of the field of view Open the field diaphragm enough to make it just disappear behind the edge of the field of vie...

Страница 125: ...cotton cloth and the special optical cleanser L This cleanser is made of 90 petroleum ether by volume and 10 isopropyl alcohol IPA by volume The ingredients are also known by the following names Petr...

Страница 126: ...are not exceeded 5 2 2 Replacing the fuses in the stand CAUTION Always disconnect the instrument from the power supply before changing any fuses If fuses blow the cause must be identified and any tec...

Страница 127: ...any technical defect must be properly repaired The fuse compartment is on the back of the power supply unit and contains two fuses of the type T 5 A H 250 V 5x20 mm Disconnect the power plug Pull the...

Страница 128: ...e filter correctly Low resolution and poor contrast Opening of the aperture diaphragm is not adjusted correctly Adjust the opening of the aperture diaphragm according to the 2 3 rule and the texture o...

Страница 129: ...reflect turret and make sure the correct reflector is in use The field diaphragm is closed Check and if necessary open the field diaphragm XY stage stops at wrong position after initialization on Axio...

Страница 130: ...or dust in the field of view Condenser is not focused properly and front lens is not in the correct on or off mode Focus the condenser and turn the front lens either on or off appropriately see p 87 f...

Страница 131: ...l and electronic parts inside the instrument and on the electrical equipment of the Axioscope microscopes may be performed only by the ZEISS Service Department or specially authorized technical person...

Страница 132: ...ential Interference Contrast DIN Deutsches Institut f r Normung German Institute for Standardization EC European Community EN Euronorm European Standard Ergo Ergonomic Ergonomics FL Fluorescence foc f...

Страница 133: ...rast for Plastic Receptacles Pol Polarization P C Push Click R Right hand coaxial knurled knob on the right hand side of the mechanical stage RL Reflected light SLR Single Lens Reflex T Slow type of f...

Страница 134: ...system 107 Bertrand system module 107 Binocular photo tube 36 Binocular tube 22 24 26 28 30 32 44 Binocular tube part 22 24 26 28 30 32 Birefringence 98 Brightfield 87 112 C Care 125 C DIC 116 Center...

Страница 135: ...slider reflected light 21 24 26 28 30 32 Filter slider transmitted light 21 22 24 26 28 30 Filter wheel 21 22 24 26 28 30 80 Fine adjustment 22 24 26 28 30 32 Fluorescence 122 Focusing drive 22 24 26...

Страница 136: ...Main switch 22 24 26 28 30 Maintenance 126 Mechanical stage 22 24 28 38 48 49 52 Mechanical stage 80x60 mot 56 Mechanical stage mot 30 Mercury vapor short arc lamp 123 Micro interferometry 117 Mode s...

Страница 137: ...older 38 Stage 21 22 24 26 28 30 Stage carrier 21 22 24 26 28 30 32 48 50 53 54 57 59 Stage control button 30 Stand 21 Stand column 32 43 Stand models 21 Stands keys 34 Start up 42 Stop slider 14x40 m...

Страница 138: ...components and the methods described in this manual are protected by the following patents US 6154282 US 6392796 US 6595661 US 7038847 EP 1359453 US 7046436 CN 206479707 U 6 4 System overview See nex...

Страница 139: ...for P C modules 424940 9001 000 Reflector slider 2x encoded changeable for P C modules 424940 9051 000 Reflector turret 4x encoded changeable for P C modules 424940 9061 000 Filter set D 000000 1058 2...

Страница 140: ...Stage carrier H 140 mm for Axioscope Vario base plate 451016 9902 000 Upper stand for Axioscope HAL 100 HBO 6x HD DIC M27 Dust protection set L 434304 0000 000 Stages with drive legth 140 mm can be u...

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