Zeiss Axioscope 5, Инструкция по эксплуатации

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5 Operation | 5.4 Setting Up for Transmitted Light Techniques ZEISS
Procedure
1. Swivel the DIC compatible objective
3
into the beam path.
2. Slide the according DIC slider into the slit of the appropriate objective position.
3. Swing in the analyzer module
2
on the reflector turret
1
(or slide the analyzer slider
7
into the intermediate plate for analyzer sliders).
4. On the condenser
4
, swing in the DIC prism (use position DIC ).
5. Adjust field diaphragm and aperture diaphragm according to the KÖHLER method [
}
87] .
6. With the knurled screw
6
on the DIC slider
5
, adjust the optimal contrast.
Symmetrical adjustment of the DIC slider along its middle position lets the sample details
appear as if they were elevated or deepened.
7. Put the compensator λ into the opening above the nosepiece to create a chromatic DIC
contrast, if required.
5.4.7 Setting Up for Transmitted Light PlasDIC Microscopy
Prerequisite ü The microscope is operational.
ü Abbe condenser with modulator disk and objective-dependent 2 mm slit diaphragm for Plas-
DIC (A-Plan 10x and LD A-Plan 20x) or 4.5 mm slit diaphragm for PlasDIC (in all other cases) is
installed.
ü One of the following objectives is installed [
}
67] :
A-Plan 10x, 20x, 40x;
LD A-Plan 20x, 32x, 40x;
LD Plan-Neofluar 20x, 40x, 63x
ü DIC slider, compatible with the objectives in use, is available.
ü The analyzer module ACR P&C for transmitted light in the reflector turret/slider or the analyzer
slider D/A fixed or rotatable in conjunction with a mounted intermediate plate for analyzer
slider 12x46 is installed.
Procedure 1. Fully open the aperture diaphragm of the condenser.
2. Place the sample on the stage.
3. Swing the condenser position with the 2 or 4.5 mm slit diaphragm for PlasDIC into the
beam path.
4. Increase the brightness.
5. Swing in the analyzer module on the reflector turret (or slide the analyzer slider into the in-
termediate plate for analyzer sliders).
6. Swivel the PlasDIC compatible objective into the beam path.
7. Slide the according DIC slider
1
into the slit
of the appropriate objective position.
1
2
8. With the knurled screw
2
on the DIC slider, adjust the optimal contrast.
à The structures are visible in relief or in pseudo-darkfield. The relief display provides the
best contrast.
98 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001