5 Operation | 5.6 Switching Off the Microscope
ZEISS
11. Swivel it in the FL P&C reflector module
4
with the desired fluorescence filter combina-
tion (depending on the excitation mode).
12. Unblock the light path in the reflected light light source with the filter slider for reflected
light
8
.
13. If necessary, adjust the FL attenuator to 100% transmission in order to facilitate locating
fluorescence signals.
à
Reduce the transmission later to preserve the sample.
14. Remove an eyepiece from the tube and adjust the aperture diaphragm
1
by sight.
15. Open the aperture diaphragm enough to see the whole objective exit pupil.
16. Replace the eyepiece in the tube.
17. Close the field diaphragm
3
enough to make it visible in the field of view.
18. Using both centering screws
2
, center the field diaphragm on the edge of the field of
view.
19. Open the field diaphragm enough to make it just disappear behind the edge of the field of
view, or, if you are using a sample which might bleach out, reduce the field diaphragm for
the field of view.
20. Focus the sample again.
21. If using the HBO 100 light source, optimize the collector position of the HBO 100 using the
knurled knob.
à
Adjust the collector so that the reflector module of the short-wave excitation illuminates
the field of view evenly.
à
A correction of the collector position is not necessary in modules with longer-wave exci-
tation.
9
The illumination is now adjusted for fluorescence microscopy.
5.6 Switching Off the Microscope
Procedure
1. Switch the microscope off using the
power
switch On/Off
.
2. Cover the microscope with the dust cover.
116
Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001
