Zeiss Axioscope 5, Инструкция по эксплуатации

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5 Operation | 5.4 Setting Up for Transmitted Light Techniques ZEISS
9. For the detection of gout or pseudo-gout, select crystal needles that are oriented in the
gamma direction (see marking on the lambda plate).
à
If the crystal needles oriented parallel to the gamma direction of the lambda plate are
yellow, and the crystal needles lying at a right angle to the gamma direction are blue, the
crystals are monosodium urate crystals (gout).
à
If the crystal needles oriented parallel to the gamma direction of the lambda plate are
blue, and the crystal needles lying at a right angle to the gamma direction are yellow, the
crystals are calcium pyrophosphate crystals (pseudo-gout).
5.4.8.5 Transmitted Light Polarization for Conoscopic Observation
For more information about the conoscopic observation, see chapter Transmitted Light Polariza-
tion for Coniscopic Observation [
}
59] .
5.4.8.5.1 Simple Conoscopy Using the Auxiliary Microscope or the Diopter
Prerequisite ü Strain-free objectives are installed in the nosepiece [
}
67] .
N-Achroplan 50x/0.9 Pol objective or EC Plan-Neofluar 40x/0.9 Pol objective
ü
The Pol rotary stage is installed [
}
159] .
ü Pol binocular photo tube is installed or eyepiece with crossline micrometer 14:140 and adjust-
ment aid for polarization microscopy are available.
ü
The achromatic-aplanatic 0.9 H Pol condenser or 0.9 Pol condenser is installed.
ü The D polarizer (rotatable or fixed) is installed.
ü
Analyzer slider or D Pol analyzer module in the reflector turret or reflector slider are available.
ü The microscope is adjusted for transmitted light brightfield microscopy [
}
87] .
Procedure
1. Place the sample on the stage.
2. Focus the sample.
3. Swivel the polarizer.
4. Move the analyzer into the light path.
5. If you do not use the Pol binocular photo tube, carry out the following two steps:
− Align the crossline micrometer 14:140 or the eyepiece reticle to the vibration direction of
the polarizer using the
adjustment aid [
}
100] .
− Remove the Pol adjustment aid.
6. Move a selected crystal to the center of the reticle. Only crystals above a defined size can
be observed.
7. Swivel in the front lens on the condenser, if necessary.
8. For conoscopy of small crystals, close the luminous-field diaphragm, if necessary, to prevent
the axial figure of the examined crystal from being superimposed by the axial figures of ad-
jacent crystals.
9. Swivel the 40x or 50x objective into the light path.
10. Focus the sample.
11. Remove an eyepiece from the tube to view the axial figure in the eyepiece tube.
12. For an improved assessment of the axial figure, insert a diopter or an auxiliary microscope
(if available) in the eyepiece tube.
102 Instruction Manual ZEISS Axioscope 5, Axioscope 5/7 MAT | en-US | Rev. 13 | 430035-7344-001