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42
Contrast Methods and Filter Sets
Whether selecting a contrast method or a filter set the following table presents only
manual operations that should be avoided with ZEN 2.1 software in most of the cases.
Contrast Method/
Filter Set
Symbol/
Number
Control Device
Instruction
Bright Field
BF/4
Setting Wheel/ Fluar
Illuminator Z mot./
Diaphragm Slide
In the Fluar Illuminator Z mot., press the number
4
.
Next, turn the setting wheel towards the position
marked with
BF.
Open the diaphragm slide
Higher Contrast
Bright Field
BF+/4
Setting Wheel/ Fluar
Illuminator Z mot./
Diaphragm Slide
In the Fluar Illuminator Z mot., press the number
4
.
Next, turn the setting wheel towards the position
marked with
BF+.
Open the diaphragm slide
Dark Field
DF/4
Setting Wheel/ Fluar
Illuminator Z mot./
Diaphragm Slide
In the Fluar Illuminator Z mot., press the number
4
.
Next, turn the setting wheel towards the position
marked with
DF.
Open the diaphragm slide
Fluorescence: GFP
1
Fluar Illuminator Z
mot.
Cover the transmitted light illumination by moving the
diaphragm slide towards the front stop position. In
the Fluar Illuminator Z mot., press the number 1.
Fluorescence: YFP
2
Fluar Illuminator Z
mot.
Cover the transmitted light illumination by moving the
diaphragm slide towards the front stop position. In
the Fluar Illuminator Z mot., press the number 1.
Fluorescence:
mRFP
3
Fluar Illuminator Z
mot.
Cover the transmitted light illumination by moving the
diaphragm slide towards the front stop position. In
the Fluar Illuminator Z mot., press the number 1.