Operating Instructions for Zefon Biopump
Calibration (in the field):
1. Press the On/Off button to turn on the pump.
2. Use the Up/Down arrow buttons to select CAL.
3. Connect the end of the flow indicator to the Bio-Pump.
4. Press the Set button and the pump will start running at low speed.
5. Press the Up/Down arrow buttons rapidly to adjust the flow rate until the silver ball in the flow indicator is in the
middle of the black (15 L) line.
6. Press the Set button to save the calibration. The pump will stop automatically.
7. Press Up/Down arrow button to select Verify Calibration.
8. Press Start/Stop to begin verification. The silver ball should reach the middle of the black line and remain there.
9. Press the Start/Stop to end verification.
10. Remove the flow indicator.
Spore trap collection:
1. Calibrate the pump prior to sampling.
2. Determine sampling sites.
Collect an indoor sample from an unaffected area to serve as a control.
Collect a representative outdoor sample to provide a reference for determining whether certain fungiare
being amplified in the indoor environment.
Submit a blank, unexposed spore trap cassette with each sampling event to serve as a negative control.
3. Place the pump in the desired location.
4. Label the spore trap cassette with sample number.
Do not use damaged or expired cassettes.
Avoid writing over the lot number and expiration date on the cassette.
5. Remove the label from the outlet port of the cassette and place it on the side of the pump.
T
he labels on the outlet and intake ports will be used later to seal the cassette
.
6. Place the cassette on the rubber grommet on top of the pump until it fits snugly.
7. Immediately before sampling, remove the spore trap label from the inlet of the cassette and place it on the side of
the pump.
8. Examples of recommended sampling times:
Sampling volume (15 L/min); 75 total liters is recommended as a benchmark for representative sample
volume.
The flow rate recommended for total spore sampling is 15L/min for 3-5 minutes with Air-O-Cell, VersaTrap,
and Allergenco-D cassettes, and 5L/min for 5 minutes with Micro-5 cassettes. Longer sampling times are
used for clean rooms, hospitals, and remediated areas.
In dusty or heavily polluted areas, particle overloading may occur, and sampling times/volume should be
adjusted accordingly.
9. Turn on the pump.
The impacted area (trace) of the spore trap should appear opaque, not white, when an appropriate
amount of sample has been collected.
If the trace appears white, repeat the sample collection for a shorter amount of time.
