120
Thoroughly clean with disinfecting agent, then rinse with warm water. Remove probes and discard enzyme membranes.
Clean enzyme probes with isopropanol only, rinse with warm water. Do not clean ISE probes unless you plan to replace
them. Clean up all spills, then reassemble.
Daily Maintenance
9.2
9.2.1 Empty the Waste Bottle(s)
Carefully pull the waste tube out of the hole in each waste bottle. Unscrew the lid from the waste bottle then lift the waste
bottle out of the bottle tray.
If the 2900 Series is used for medical research or biological testing, dispose of the waste bottle contents in a manner
suitable for biohazardous waste. The YSI reagents used in the 2900 Series are non-toxic and, unless otherwise specified,
consist of a phosphate salt buffer with small amounts of preservatives. Refer to reagent bottle labels and Material Safety
Data Sheets for more information.
Slide the waste bottle back into the bottle tray and screw the lid back onto the bottle. Insert the waste tube into the hole in
the bottle.
9.2.2 Check the Calibrator Bottle(s)
If the fluid level is low or the bottle has been in the instrument longer than the working life (as stated on the bottle), install
a new bottle of calibrator solution.
After installation, [Prime] the Calibrator. If you are calibrating from a test tube, evaporation is a major concern. Calibrating
with calibrator that has experienced any significant evaporation will cause inaccurate test results. Consider changing
calibrator daily, or more often, if required.
9.2.3 Check the Buffer Bottle(s)
Replace the buffer if the bottle is low or the buffer has been in the instrument longer than 1 week. Clean the buffer bottle
and cap with a bleach solution, then rinse well with DI water before installing fresh buffer. You may find it convenient to
make more than one liter of buffer at a time, in order to have it on hand to replenish the buffer bottle. Prepare the buffer in
a clean bottle with cap and store any excess at room temperature.
After a buffer change, [Prime] the buffer system. Buffer should be exiting the Sipper inside the sample module and
overflowing to waste. You may need to initiate a second or third run of the buffer pump to complete the priming process.
9.2.4 Check for Leaks
Examine the instrument for leaks. These are caused either by loose connections or worn tubing.
9.2.5 Clean up Spills
Spills should be cleaned up promptly to prevent biohazard build-up and corrosion. Clean any spills of biological material
from the sample area.
9.2.6 Daily Operational Checks
To verify proper instrument performance, perform the daily operational checks described in Section 5.1.1Enzyme
Membrane Integrity Test and 5.1.2 Linearity Test.
Monthly Maintenance
9.3
9.3.1 Calibration Pumping System Maintenance
Perform this procedure at least once a month to minimize the possibility of contamination. The most convenient time to
perform this maintenance is before installation of a new bottle of calibration standard.
