
11
11
11
11----2
2
2
2
Sodium fluoride and oxalate in combination in a total concentration of 2
mg/ml of blood.
Heparin sodium as commonly supplied in Vacutainer collection tubes.
WARNING: The YSI SPORT is not suitable for use with
samples containing thymol or other phenol preservatives.
These are interfering substances.
Oxalate anticoagulants, and particularly the combination of sodium fluoride and
oxalate, may produce hemolysis, which can cause disagreement between results
from the SPORT and some reference methods.
Warning: Frozen blood-bank blood, which contains a high
percentage of glycerine, is not a suitable specimen for
diagnostic work with the SPORT.
Blood Serum
Blood Serum
Blood Serum
Blood Serum
Draw blood for serum preparation into a dry tube without additives. Silicone-
coated tubes and stoppers lubricated with glycerine or silicone are acceptable.
After the specimen stands 15 minutes at room temperature, loosen the clot from
the wall of the tube with a sterile glass rod or applicator. Add sterile beads, if
desired. Centrifuge the specimen promptly to separate the serum from the clot.
If delays are unavoidable follow one these procedures:
1. Refrigerate the whole blood at +4°C. This is acceptable for periods of up to
4 hours.
2. Or, prolonged storage, isolate the serum from the clot within 30 minutes of
collection and freeze it at -10°C or colder. Serum will keep indefinitely at
this temperature. Thaw the frozen serum rapidly at 25°C and analyze
promptly. The freeze-thaw cycle should not be repeated. Tubes containing
cells should never be frozen.
NOTE:
NOTE:
NOTE:
NOTE: Freezing may precipitate aggluten or fibrinogen masses which may
not redissolve. You can remove these by centrifugation before assay.
3. Or by adding a chemical preservative. Preservatives are not commonly used
with serum; however, sodium fluoride in concentrations of 7.5 mg/ml of
blood, EDTA in concentrations of 1 mg/ml of blood, or boric acid in
concentrations of 8 mg/ml of blood will substantially halt glycolysis in
most, but not all, specimens. If sodium iodoacetate is used as an
antiglycolytic, it must be free of iodide and iodine.
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